diff --git a/pyproject.toml b/pyproject.toml
index ea99c508a..2b4c6f6c7 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -35,7 +35,7 @@ dependencies = [
   "tifffile",
   "python-multipart",
   "future",
-  "pynwb==2.2.0",
+  "pynwb==2.3.3",
   "psutil",
   "nbstripout",
   "snakemake>=7.24,<7.31",