LCCD not robust to short videos with default settings

[milesAraya]

追加機能についての概要
LCCD only works with videos > 300 frames with defaults.

Should robustly check parameters based on the rules in ()

• lccd_detect
  • Description: Low Computational-cost Cell Detection
  • Input: ImageData
  • Output: FluoData (time series for each detected cell), RoiData (spatial masks for each detected cell)
  • Parameters:
    • blob_detector: Initial step that identifies potential cells or "blobs" in each frame of the video.
      • filtersize1 [int (filtersize1 <= Time / 2), default: 100]: Size of the larger temporal filter for smoothing.
      • filtersize2 [int (filtersize2 < filtersize1), default: 4]: Size of the smaller temporal filter for edge detection.
      • sigma [float, smaller than T & filtersize2 < filtersize1, default: 1.25]: Standard deviation for the Gaussian filter.
      • fsize [int (odd number recommended), default: 9]: Size of the 2D Gaussian filter for spatial filtering.
      • min_area [int (min_area < max_area < X * Y), default: 16]: Define the acceptable size range for detected blobs.
      • max_area [int, (max_area < X * Y), default: 100]: Define the acceptable size range for detected blobs.
      • sparse [bool, default: False]: Determines whether to use sparse matrix operations.
  • roi_integration: Takes blobs detected across all frames and integrates them into coherent ROI that represent consistent cell locations over time.
    • overlap_threshold [float (0 < overlap_threshold < 1), default: 0.25]: Threshold for determining significant overlap between ROIs
    • min_area [int (min_area < max_area < X * Y), default: 16]: Define the acceptable size range for integrated ROIs.
    • max_area [int (max_area < X * Y), default: 100]: Maximum acceptable size for integrated ROIs.
    • sparse [bool, default: False]: Determines whether to use sparse matrix operations.
  • lccd:
    • frame_divider [int (Time / frame_divider >= 2), default: 100]: Divides the time dimension into segments for faster processing.
  • dff:
    • f0_frames [int (0, f0_frames, Time), default: 100]: Number of frames used to calculate the baseline fluorescence.
    • f0_percentile [float (0 <= f0_percentile <= 100), default: 8]: Percentile used for calculating the baseline fluorescence.

重要度
この機能の重要度を[低、中、高]に分ける。
低: この機能は開発要件の最低条件に含まれていない。