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Module 1: Webin command line submission interface

Introduction

Submissions to ENA can be made using the interactive Webin submission service, programmatic Webin submission service and the Webin command line submission service.

This module gives an introduction to the Webin command line submission interface used to validate, upload and submit files to the European Nucleotide Archive (ENA).

Registration

If you have not submitted to Webin before please register a submission account.

Submission process

The following types of submissions are supported:

  • genome assemblies
  • transcriptome assemblies

The type of the submission is specified using the -context command line option:

  • -context genome
  • -context transcriptome

The following picture illustrates the stages of the submission process:

Submission process

Stage 1: Pre-register study and sample

Each submission must be associated with a pre-registered study and a sample. The study and sample accessions or unique names (aliases) are provided in an info file associated with the submission.

Instructions for interactive submitters:

Instructions for programmatic submitters:

Stage 2: Prepare the files

The set of files that are part of the submission must be specified using a manifest file. The manifest file is specified using the -manifest <filename> option.

Manifest file format

The manifest file in a text file (USASCII7) with two columns separated by a tab (or any whitespace characters):

  • File type (first column): case insensitive file type.
  • File path (second column): the path to the file.

For example, the following manifest file represents a genome assembly consisting of an info file and a fasta file:

INFO    genome.info.gz
FASTA   genome.fasta.gz

Manifest file types

The following case-insensitive file types are supported within a manifest file:

  • INFO: An info file containing tab-separated key value pairs
  • FASTA (genome and transcriptome assemblies): Sequences in fasta format
  • FLATFILE (genome and transcriptome assemblies): Sequences in EMBL-Bank flat file format
  • AGP (genome assemblies): See Genome Assembly Submissions
  • CHROMOSOME_LIST (genome assemblies): See Genome Assembly Submissions
  • UNLOCALISED_LIST (genome assemblies): See Genome Assembly Submissions

Stage 3: Validate and submit files

You can validate your files using the -validate command line option. Note that the -submit option must be used to submit the validated files.

You can submit your files using the -submit command line option. Before being submitted your files will be validated and uploaded to your private Webin file upload area in webin.ebi.ac.uk.

Please refer to the following documents for validation rules:

Validation error reports are written into the <outputDir>/<context>/<name>/validate directory.

The webin command line submission interface creates and submits XMLs for you. These XMLs and the Receipt XML containing accession numbers are written into the <outputDir>/<context>/<name>/submit directory. This directory also contains the file manifest that refers to the files that are part of the submission.

The <outputDir> can be specified using the -outputDir option, the <context> is specified using the -context option, and the <name> is the submitter provided unique name specified in the info file (e.g. genome or transcriptome assembly name).

Once the submission is complete an accession number is immediately returned to the submitter by the Webin command line submission interface. Please refer to the following documents for advice which long term stable accession numbers can be used in publications:

Download the program

The latest version of the Webin command line submission interface can be downloaded from:

https://github.com/enasequence/webin-cli/releases

The program requires Java 1.8 or newer which can be downloaded from:

https://java.com/en/download/

Using the program

The Webin command line submission interface is a self-executing Java jar file and is run using the java command:

java -jar webin-cli-<version>.jar <options>

The <version> is the version number of the program.

The command line <options> are explained below.

Command line options

  • -context: the submission type:
    • -context genome
    • -context transcriptome
  • -userName: the Webin submission account name.
  • -password: the Webin submission account password.
  • -manifest: the manifest file name.
  • -outputDir: directory for output files.
  • -validate: validates the files defined in the manifest file.
  • -submit: validates and submits the files defined in the manifest file.
  • -test: use Webin test service instead of the production service. Please note that the Webin upload area is shared between test and production services, and that test submission files will not be archived.
  • -help: detailed information about the different options.

Output directory structure

An output directory can be specific to the Webin command line submission interface using the -outputDir option. This directory will have the following subdirectories:

  • <context>/<name>/validate
  • <context>/<name>/submit

If the -outputDir option is not specified then the directory in which the -manifest file is used as the output directory.

The <context> is the submission type provided using the -context option and the <name> is the unique name provided in the info file.

  • The validate directory contains the validation reports created using the -validate option.
  • The submit directory contains the XMLs created by the -submit option including the Receipt XML. This directory also contains the file manifest that refers to the files that are part of the submission.

Validation reports

If the -validate action fails for any reason then validation reports are written into directory:

<context>/<name>/validate

The validation reports correspond to the input files with an added suffix .report.

For example, a validated file assembly.info will have a corresponding validation report assembly.info.report, and a validated fasta file assembly.fasta will have a corresponding validation report assembly.fasta.report.

Messages which can't be attributed to a specific input file will be written to both standard out and in the following file:

<context>/<name>/validate/webin-cli.report

Proxy servers

If your organisation uses a https proxy you can set the following Java properties to instruct the webin-cli to use them:

  • https.proxyHost
  • https.proxyPort

For example:

java -Dhttps.proxyHost=proxy.com -Dhttps.proxyPort=8080 -jar webin-cli-<version>.jar <options>

Similarly, if your organisation uses a ftp proxy you can set the following properties:

  • ftp.proxyHost
  • ftp.proxyPort

For example:

java -Dftp.proxyHost=proxy.com -Dftp.proxyPort=8080 -jar webin-cli-<version>.jar <options>