My png image has a transparent "hole" but won't show up as transparent

[HightonRidley]

I have a png image with a hole that's transparent. When I add it as a frame, the hole is no longer transparent. How can I make it so it's transparent?
I've tried setting transparent: "rgba(0,0,0,0)" and also transparent: "#0x00000000" but neither work, eg:
gif = new GIF({
workerScript: "js/gif-maker-lib/gif.worker.js",
repeat: 0,
workers: 2,
width: 695,
height: 695,
transparent: "#0x00000000",
quality: 10
});

[cmann1]

"#0x00000000" doesn't really make sense.
A CSS style hex string should be in a string in the form "#RRGGBB", eg. "#FF00FF"
And a "JS" style colour value should be 0xRRGGBB (this is just another way to represent an integer, so no quotes), eg. 0xFF00FF

The "transparent" option expects an integer, so change that line to
transparent: 0x00000000,