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Potential new cell types & mappings for whole human brain scRNAseq dataset #1980

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dosumis opened this issue May 18, 2023 · 5 comments
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@dosumis
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dosumis commented May 18, 2023

Paper: https://doi.org/10.1101/2022.10.12.511898 Siletti et al., 2022 bioRxiv Transcriptomic diversity of cell types across the adult human brain
supplementary-material

Supplementary material table 2 has two columns that are potentially mappable to cell types:

Supercluster - Shawn worked on mapping and adding terms - see #1743 for details. This work is mostly done but it might be worth following up the unchecked items on Shawn's list - particularly grouping of types derived from upper and lower rhombic lip.

Subtype auto-annotation: Review contents of this column - referring back to text of paper to work out if we should add a new cell type to CL or whether we have an existing type that could be mapped to.

This is a uniq'd list of entries in Subtype auto-annotation column + some potential mappings. But note - it can be useful to look at the context of the rest of the row in table 1 to work out what is being referred to.

Table entry Mapping
ABC PIA
ARACH
ARTERIOLE ARTERY
ARTERIOLE CAP
CAP
CB-GRAN cerebellar granule cell (CL:0001031)
CB-GranLayerInt
CB-MolLayerInt1
CB-MolLayerInt1 CB-MolLayerInt2
CB-MolLayerInt2
CB-PURK Purkinje cell (CL:0000121)
CB-PurkLayerInt
CHAND chandelier cell (CL:4023083)
COP
DG-GRAN TBA dentate gyrus granule cell (have not found detail in paper)
FIBMYO
INT-LAMP5
INT-LAMP5-LHX6
INT-PVALB
INT-SST
INT-SST INT-SST-CHODL
INT-SST-CHODL
INT-VIP
L5-ET L5 extratelencephalic projecting glutamatergic cortical neuron (CL:4023041)
L5-ET VonEcon TBA - von Economo neuron
MSN-D1
MSN-D1 MSN-D2 MSN-ECC
MSN-D1 MSN-ECC
MSN-D2
PIA
PIA PVF
UBC
VENOUS
@dosumis dosumis assigned ghost May 18, 2023
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dosumis commented May 18, 2023

CC @hkir-dev

Note - it may be worth contacting Siletti to clear up any ambiguities once a first pass at mapping has been done.

@dosumis
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dosumis commented May 23, 2023

@bvarner-ebi - have made as start at this as want to some example mappings for a demo.

This is a uniq'd list of entries in Subtype auto-annotation column + some potential mappings:

Table entry Mapping
ABC PIA
ARACH
ARTERIOLE ARTERY
ARTERIOLE CAP
CAP
CB-GRAN cerebellar granule cell (CL:0001031)
CB-GranLayerInt
CB-MolLayerInt1
CB-MolLayerInt1 CB-MolLayerInt2
CB-MolLayerInt2
CB-PURK Purkinje cell (CL:0000121)
CB-PurkLayerInt
CHAND
COP
DG-GRAN TBA -
FIBMYO
INT-LAMP5
INT-LAMP5-LHX6
INT-PVALB
INT-SST
INT-SST INT-SST-CHODL
INT-SST-CHODL
INT-VIP
L5-ET
L5-ET VonEcon TBA - von Economo neuron
MSN-D1
MSN-D1 MSN-D2 MSN-ECC
MSN-D1 MSN-ECC
MSN-D2
PIA
PIA PVF
UBC
VENOUS
  • NTR - von Economo Neuron
  • ...

@ghost
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ghost commented May 23, 2023

This comment is in progress.
Template copied from comment above.

Table entry Mapping
ABC PIA ?fibroblast in pia mater
ARACH ?fibroblast in arachnoid mater
ARTERIOLE ARTERY CL:1000412 'endothelial cell of arteriole'
ARTERIOLE CAP CL:1000412 'endothelial cell of arteriole' | CL:002144 'capillary endothelial cell'
CAP CL:0002144 'capillary endothelial cell'
CB-GRAN CL:0001031 'cerebellar granule cell'
CB-GranLayerInt ?NTR- cerebellar granular layer interneuron
CB-MolLayerInt1 ?NTR- cerebellar molecular layer interneuron 1
CB-MolLayerInt1 CB-MolLayerInt2 -
CB-MolLayerInt2 ?NTR- cerebellar molecular layer interneuron 2
CB-PURK CL:0000121 'Purkinje cell'
CB-PurkLayerInt ?NTR- cerebellar purkinje layer interneuron
CHAND CL:4023083 'chandelier cell'
COP CL:4023059 'differentiation-committed oligodendrocyte precursor'
DG-GRAN CL:4023062 'dentate gyrus neuron' | CL:2000089 'dentate gyrus of hippocampal formation granule cell'
FIBMYO CL:0002590 'smooth muscle cell of the brain vasculature' | CL:0000186 myofibroblast
INT-LAMP5 CL:4023070 'caudal ganglionic eminence derived GABAergic cortical interneuron' | CL:4023011 'lamp5 GABAergic cortical interneuron'
INT-LAMP5-LHX6 ? check if this warrants a new term
INT-PVALB CL:4023069 'medial ganglionic eminence derived GABAergic coritcal interneuron' | CL:4023018 'pvalb GABAergic cortical interneuron'
INT-SST CL:4023069 'medial ganglionic eminence derived GABAergic coritcal interneuron' | CL:4023017 'sst GABAergic cortical interneuron'
INT-SST INT-SST_CHODL -
INT-SST-CHODL CL:4023069 'medial ganglionic eminence derived GABAergic coritcal interneuron' | CL:4023121 'sst chodl GABAergic cortical interneuron'
INT-VIP CL:4023070 'caudal ganglionic eminence derived GABAergic cortical interneuron' | CL:4023016 'vip GABAergic cortical interneuron'
L5-ET CL:4023041 'L5 extratelencephalic projecting glutamatergic cortical neuron'
L5-ET VonEcon NTR done: CL:4030039 'von Economo neuron'
MSN-D1 CL:4023026 'direct pathway medium spiny neuron'
MSN-D1 MSN-D2 MSN-ECC -
MSN-D1 MSN-ECC -
MSN-D2 CL:4023029 'indirect pathway medium spiny neuron'
MSN-ECC NTR done: CL:4030057 'eccentric medium spiny neuron'
PIA ?fibroblast in pia mater
PIA PVF ?fibroblast in pia mater
UBC ?
VENOUS CL:0002543 'vein endothelial cell'

@dosumis dosumis added the BICAN label May 24, 2023
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dosumis commented Jun 19, 2023

Next steps:
@bvarner-ebi to work with @hkir-dev to get these mappings into the while human brain PCL build.
@dosumis to contact Siletti to suggest meeting to discuss further & ask if she is happy to provide answers to more questions.

@ghost
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ghost commented Jun 22, 2023

@hkir-dev, can you comment on the mappings above and if you need further input from me?

Notes on mappings:
Question marks (?) signify best guesses.
Hyphens (-) are next to aggregates of other entries - I don't have a recommendation on how those should be mapped.
Otherwise, if there is no question mark, those terms can be mapped to the dataset entries.

My understand is that @dosumis would like to move forward using the confident mappings (those with no "?").

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