From 04092ef23e286acceda3790c146f6f075f56114b Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 11 Dec 2023 11:24:27 -0600 Subject: [PATCH 001/113] Indexing and finding repeats. --- conf/modules.config | 8 ---- modules/local/assemblystub/main.nf | 33 ---------------- modules/local/findrepeats/main.nf | 23 +++++++++++ modules/local/indexing/main.nf | 27 +++++++++++++ nextflow.config | 1 + nextflow_schema.json | 7 +++- tests/modules/local/assemblystub/main.nf.test | 38 ------------------- workflows/snvphylnfc.nf | 34 ++++------------- 8 files changed, 65 insertions(+), 106 deletions(-) delete mode 100644 modules/local/assemblystub/main.nf create mode 100644 modules/local/findrepeats/main.nf create mode 100644 modules/local/indexing/main.nf delete mode 100644 tests/modules/local/assemblystub/main.nf.test diff --git a/conf/modules.config b/conf/modules.config index 1fbf485..cbe33ab 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -30,14 +30,6 @@ process { ] } - withName: ASSEMBLY_STUB { - publishDir = [ - path: { ["${params.outdir}", "${task.assembly_directory_name}"].join(File.separator) }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: GENERATE_SUMMARY { publishDir = [ path: { ["${params.outdir}", "${task.summary_directory_name}"].join(File.separator) }, diff --git a/modules/local/assemblystub/main.nf b/modules/local/assemblystub/main.nf deleted file mode 100644 index 00f27d2..0000000 --- a/modules/local/assemblystub/main.nf +++ /dev/null @@ -1,33 +0,0 @@ -process ASSEMBLY_STUB { - tag "$meta.id" - label 'process_single' - - container 'docker.io/python:3.9.17' - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.assembly.fa.gz"), emit: assembly - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - cat <<-EOF > ${prefix}.assembly.fa - >${meta.id}-stub-assembly - ACGTAACCGGTTAAACCCGGGTTTAAAACCCCGGGGTTTTAAAAACCCCCGGGGGTTTTT - EOF - - gzip -n ${prefix}.assembly.fa - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - assemblystub : 0.1.0 - END_VERSIONS - """ -} diff --git a/modules/local/findrepeats/main.nf b/modules/local/findrepeats/main.nf new file mode 100644 index 0000000..098d642 --- /dev/null +++ b/modules/local/findrepeats/main.nf @@ -0,0 +1,23 @@ +/* Find repeats and create invaild_positions.bed */ +process FIND_REPEATS { + tag "${refgenome}" + label 'process_low' + container = "staphb/snvphyl-tools:1.8.2" + + input: + path(refgenome) + + output: + path("invalid_positions.bed"), emit: repeats_bed_file + path("versions.yml"), emit: versions + + script: + """ + find-repeats.pl ${refgenome} --min-length 150 --min-pid 90 > invalid_positions.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + snvphyl-tools: + perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) + """ +} \ No newline at end of file diff --git a/modules/local/indexing/main.nf b/modules/local/indexing/main.nf new file mode 100644 index 0000000..cb7d368 --- /dev/null +++ b/modules/local/indexing/main.nf @@ -0,0 +1,27 @@ +process INDEXING { + tag "${refgenome}" + label 'process_low' + container = "staphb/smalt:0.7.6" + + input: + path(refgenome) + + output: + path('*.fai'), emit: ref_fai + path('*.sma'), emit: ref_sma + path('*.smi'), emit: ref_smi + path("versions.yml"), emit: versions + + script: + """ + REF_BASENAME=\$(basename ${refgenome} .fasta) + smalt index -k 13 -s 6 \${REF_BASENAME} ${refgenome} + samtools faidx ${refgenome} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + smalt: \$( smalt version | grep "Version:" | sed 's/Version://' ) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 7f956c4..05cfc38 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,6 +11,7 @@ params { // Input options input = null + refgenome = "${baseDir}/assets/reference.fasta" // Boilerplate options outdir = null diff --git a/nextflow_schema.json b/nextflow_schema.json index f2618ac..493c591 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -206,5 +206,10 @@ "$ref": "#/definitions/generic_options" } ], - "properties": {} + "properties": { + "refgenome": { + "type": "string", + "default": "/home/eric/projects/snvphylnfc/assets/reference.fasta" + } + } } diff --git a/tests/modules/local/assemblystub/main.nf.test b/tests/modules/local/assemblystub/main.nf.test deleted file mode 100644 index 881bf56..0000000 --- a/tests/modules/local/assemblystub/main.nf.test +++ /dev/null @@ -1,38 +0,0 @@ -nextflow_process { - - name "Test Process ASSEMBLY_STUB" - script "modules/local/assemblystub/main.nf" - process "ASSEMBLY_STUB" - - test("Basic execution, check output.") { - - when { - params { - outdir = "tests/results" - } - process { - """ - input[0] = new Tuple(["id": "SAMPLE1"], [file("sample1_R1.fastq.gz"), file("sample1_R2.fastq.gz")]) - """ - } - } - - then { - assert process.success - - with(process.out) { - // check if emitted output has been created - assert assembly.size() == 1 - - // parse assembly file - def assembly_header = path(assembly.get(0)[1]).linesGzip[0] - def assembly_body = path(assembly.get(0)[1]).linesGzip[1] - - assert assembly_header.equals(">SAMPLE1-stub-assembly") - assert assembly_body.equals("ACGTAACCGGTTAAACCCGGGTTTAAAACCCCGGGGTTTTAAAAACCCCCGGGGGTTTTT") - } - } - - } - -} diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index f947000..5ff1eda 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -34,8 +34,9 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' include { GENERATE_SAMPLE_JSON } from '../modules/local/generatesamplejson/main' include { SIMPLIFY_IRIDA_JSON } from '../modules/local/simplifyiridajson/main' include { IRIDA_NEXT_OUTPUT } from '../modules/local/iridanextoutput/main' -include { ASSEMBLY_STUB } from '../modules/local/assemblystub/main' include { GENERATE_SUMMARY } from '../modules/local/generatesummary/main' +include { INDEXING } from '../modules/local/indexing/main' +include { FIND_REPEATS } from '../modules/local/findrepeats/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -67,34 +68,15 @@ workflow SNVPHYL { fastq_2 ? tuple(meta, [ file(fastq_1), file(fastq_2) ]) : tuple(meta, [ file(fastq_1) ])} - ASSEMBLY_STUB ( - input + INDEXING( + params.refgenome ) - ch_versions = ch_versions.mix(ASSEMBLY_STUB.out.versions) + ch_versions = ch_versions.mix(INDEXING.out.versions) - // A channel of tuples of ({meta}, [read[0], read[1]], assembly) - ch_tuple_read_assembly = input.join(ASSEMBLY_STUB.out.assembly) - - GENERATE_SAMPLE_JSON ( - ch_tuple_read_assembly - ) - ch_versions = ch_versions.mix(GENERATE_SAMPLE_JSON.out.versions) - - GENERATE_SUMMARY ( - ch_tuple_read_assembly.collect{ [it] } - ) - ch_versions = ch_versions.mix(GENERATE_SUMMARY.out.versions) - - SIMPLIFY_IRIDA_JSON ( - GENERATE_SAMPLE_JSON.out.json - ) - ch_versions = ch_versions.mix(SIMPLIFY_IRIDA_JSON.out.versions) - ch_simplified_jsons = SIMPLIFY_IRIDA_JSON.out.simple_json.map { meta, data -> data }.collect() // Collect JSONs - - IRIDA_NEXT_OUTPUT ( - samples_data=ch_simplified_jsons + FIND_REPEATS( + params.refgenome ) - ch_versions = ch_versions.mix(IRIDA_NEXT_OUTPUT.out.versions) + ch_versions = ch_versions.mix(FIND_REPEATS.out.versions) CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 45681362333e956d07140d132ad70e613537201b Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 11 Dec 2023 11:36:39 -0600 Subject: [PATCH 002/113] smalt --- modules/local/smaltmap/main.nf | 29 +++++++++++++++++++++++++++++ workflows/snvphylnfc.nf | 6 ++++++ 2 files changed, 35 insertions(+) create mode 100644 modules/local/smaltmap/main.nf diff --git a/modules/local/smaltmap/main.nf b/modules/local/smaltmap/main.nf new file mode 100644 index 0000000..16b0b4b --- /dev/null +++ b/modules/local/smaltmap/main.nf @@ -0,0 +1,29 @@ +/* Map reads to reference genome & create BAM file */ +process SMALT_MAP { + tag "$meta.id" + label 'process_single' + container = "staphb/smalt:0.7.6" + + input: + tuple val(meta), path(reads) + path(ref_fai) + path(ref_sma) + path(ref_smi) + + output: + tuple val(meta), path("${meta.id}.bam"), emit: bams + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + REFNAME=\$(basename ${ref_sma} .sma) + smalt map -f bam -n 4 -l pe -i 1000 -j 20 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]} ${reads[1]} + smalt version + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + smalt: \$( smalt version | grep "Version:" | sed 's/Version://' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 5ff1eda..7f19b6b 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -37,6 +37,7 @@ include { IRIDA_NEXT_OUTPUT } from '../modules/local/iridanextoutput/main' include { GENERATE_SUMMARY } from '../modules/local/generatesummary/main' include { INDEXING } from '../modules/local/indexing/main' include { FIND_REPEATS } from '../modules/local/findrepeats/main' +include { SMALT_MAP } from '../modules/local/smaltmap/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -78,6 +79,11 @@ workflow SNVPHYL { ) ch_versions = ch_versions.mix(FIND_REPEATS.out.versions) + SMALT_MAP( + input, INDEXING.out.ref_fai, INDEXING.out.ref_sma, INDEXING.out.ref_smi + ) + ch_versions = ch_versions.mix(SMALT_MAP.out.versions) + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From ca3595f3d37b000a5b4f33b218f0b2ceec34da6e Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 11 Dec 2023 11:53:22 -0600 Subject: [PATCH 003/113] sorting BAMs --- modules/local/generateline1/main.nf | 23 +++++++++++++++++++++++ modules/local/sortindexbams/main.nf | 26 ++++++++++++++++++++++++++ workflows/snvphylnfc.nf | 12 ++++++++++++ 3 files changed, 61 insertions(+) create mode 100644 modules/local/generateline1/main.nf create mode 100644 modules/local/sortindexbams/main.nf diff --git a/modules/local/generateline1/main.nf b/modules/local/generateline1/main.nf new file mode 100644 index 0000000..495bdd5 --- /dev/null +++ b/modules/local/generateline1/main.nf @@ -0,0 +1,23 @@ +/* Generate a line for the next process */ +process GENERATE_LINE_1 { + label 'process_low' + container 'quay.io/jvhagey/phoenix:base_v1.0.0' + + input: + path(sorted_bams) + + output: + path("bam_line.txt"), emit: bam_lines_file + //path("versions.yml"), emit: versions + + shell: + ''' + #! /bin/bash -l + count=0 + for f in *_sorted.bam + do + ((count++)) + echo "--bam bam\$count=./$f " | tr -d "\n" >> bam_line.txt + done + ''' +} \ No newline at end of file diff --git a/modules/local/sortindexbams/main.nf b/modules/local/sortindexbams/main.nf new file mode 100644 index 0000000..315ab58 --- /dev/null +++ b/modules/local/sortindexbams/main.nf @@ -0,0 +1,26 @@ +/* Create BAMs and Sort */ +process SORT_INDEX_BAMS { + tag "$meta.id" + label 'process_low' + container = "staphb/samtools:1.9" + + input: + tuple val(meta), path(bams) + + output: + tuple val(meta), path( "${meta.id}_sorted.bam" ), emit: sorted_bams_and_sampleID + path( "${meta.id}_sorted.bam" ), emit: sorted_bams + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + samtools sort -O bam -o ${prefix}_sorted.bam ${bams} + samtools index ${prefix}_sorted.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} \ No newline at end of file diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 7f19b6b..64bd65c 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -38,6 +38,8 @@ include { GENERATE_SUMMARY } from '../modules/local/generatesummary/main' include { INDEXING } from '../modules/local/indexing/main' include { FIND_REPEATS } from '../modules/local/findrepeats/main' include { SMALT_MAP } from '../modules/local/smaltmap/main' +include { SORT_INDEX_BAMS } from '../modules/local/sortindexbams/main' +include { GENERATE_LINE_1 } from '../modules/local/generateline1/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -84,6 +86,16 @@ workflow SNVPHYL { ) ch_versions = ch_versions.mix(SMALT_MAP.out.versions) + SORT_INDEX_BAMS( + SMALT_MAP.out.bams + ) + ch_versions = ch_versions.mix(SORT_INDEX_BAMS.out.versions) + + // TODO: Review this module further (purpose, version, code injection, container, etc.) + GENERATE_LINE_1( + SORT_INDEX_BAMS.out.sorted_bams.collect() + ) + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From b96cb03167f63e170637413c06d3b050ff10770c Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 11 Dec 2023 14:24:04 -0600 Subject: [PATCH 004/113] freebayes --- modules/local/freebayes/main.nf | 25 +++++++++++++++++++++++++ modules/local/verifyingmapq/main.nf | 25 +++++++++++++++++++++++++ workflows/snvphylnfc.nf | 12 ++++++++++++ 3 files changed, 62 insertions(+) create mode 100644 modules/local/freebayes/main.nf create mode 100644 modules/local/verifyingmapq/main.nf diff --git a/modules/local/freebayes/main.nf b/modules/local/freebayes/main.nf new file mode 100644 index 0000000..a4b45a6 --- /dev/null +++ b/modules/local/freebayes/main.nf @@ -0,0 +1,25 @@ +/* Freebayes variant calling */ +process FREEBAYES { + tag "$meta.id" + label 'process_low' + container = "staphb/freebayes:1.3.6" + + input: + tuple val(meta), path(sorted_bams) + path(refgenome) + + output: + tuple val(meta), path( "${meta.id}_freebayes.vcf" ), emit: vcf_files + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + freebayes --bam ${sorted_bams} --ploidy 1 --fasta-reference ${refgenome} --vcf ${prefix}_freebayes.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + freebayes: \$(echo \$(freebayes --version 2>&1) | sed 's/version:\s*v//g' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf new file mode 100644 index 0000000..c5e83ec --- /dev/null +++ b/modules/local/verifyingmapq/main.nf @@ -0,0 +1,25 @@ +/* Verifying Mapping Quality */ +process VERIFYING_MAP_Q { + tag "" + label 'process_single' + container = "staphb/snvphyl-tools:1.8.2" + + input: + path(sorted_bams) + val(bam_line) + + output: + path("mappingQuality.txt"), emit: mapping_quality + path("versions.yml"), emit: versions + + script: + """ + verify_mapping_quality.pl -c 4 --min-depth 10 --min-map 80 --output mappingQuality.txt ${bam_line} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + snvphyl-tools: + perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 64bd65c..98f68de 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -40,6 +40,8 @@ include { FIND_REPEATS } from '../modules/local/findrepeats/main' include { SMALT_MAP } from '../modules/local/smaltmap/main' include { SORT_INDEX_BAMS } from '../modules/local/sortindexbams/main' include { GENERATE_LINE_1 } from '../modules/local/generateline1/main' +include { VERIFYING_MAP_Q } from '../modules/local/verifyingmapq/main' +include { FREEBAYES } from '../modules/local/freebayes/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -96,6 +98,16 @@ workflow SNVPHYL { SORT_INDEX_BAMS.out.sorted_bams.collect() ) + VERIFYING_MAP_Q( + SORT_INDEX_BAMS.out.sorted_bams.collect(), GENERATE_LINE_1.out.bam_lines_file.splitText() + ) + ch_versions = ch_versions.mix(VERIFYING_MAP_Q.out.versions) + + FREEBAYES( + SORT_INDEX_BAMS.out.sorted_bams_and_sampleID, params.refgenome + ) + ch_versions = ch_versions.mix(FREEBAYES.out.versions) + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From ee18a09ec0a49c5de5ea59379c3d658536605f47 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 11 Dec 2023 14:33:29 -0600 Subject: [PATCH 005/113] freebayes post processing --- workflows/snvphylnfc.nf | 19 +++++++++++++++++++ 1 file changed, 19 insertions(+) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 98f68de..a8a9965 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -42,6 +42,9 @@ include { SORT_INDEX_BAMS } from '../modules/local/sortindexbams/main' include { GENERATE_LINE_1 } from '../modules/local/generateline1/main' include { VERIFYING_MAP_Q } from '../modules/local/verifyingmapq/main' include { FREEBAYES } from '../modules/local/freebayes/main' +include { FILTER_FREEBAYES } from '../modules/local/filterfreebayes/main' +include { BGZIP_FREEBAYES_VCF } from '../modules/local/bgzipfreebayesvcf/main' +include { FREEBAYES_VCF_TO_BCF } from '../modules/local/freebayesvcftobcf/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -108,6 +111,22 @@ workflow SNVPHYL { ) ch_versions = ch_versions.mix(FREEBAYES.out.versions) + FILTER_FREEBAYES( + FREEBAYES.out.vcf_files + ) + ch_versions = ch_versions.mix(FILTER_FREEBAYES.out.versions) + + // TODO: Is there a better way of doing this? In the last step? + BGZIP_FREEBAYES_VCF( + FILTER_FREEBAYES.out.filtered_vcf + ) + ch_versions = ch_versions.mix(BGZIP_FREEBAYES_VCF.out.versions) + + FREEBAYES_VCF_TO_BCF( + BGZIP_FREEBAYES_VCF.out.filtered_zipped_vcf + ) + ch_versions = ch_versions.mix(FREEBAYES_VCF_TO_BCF.out.versions) + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From dce17141cdc7da7a67d380580f5f35cd433469fb Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 11 Dec 2023 14:44:43 -0600 Subject: [PATCH 006/113] bcftools, freebayes, mpileup --- modules/local/bcftoolscall/main.nf | 25 ++++++++++++++++++++++++ modules/local/bgzipfreebayesvcf/main.nf | 23 ++++++++++++++++++++++ modules/local/bgzipmpileupvcf/main.nf | 23 ++++++++++++++++++++++ modules/local/filterfreebayes/main.nf | 25 ++++++++++++++++++++++++ modules/local/freebayesvcftobcf/main.nf | 26 +++++++++++++++++++++++++ modules/local/mpileup/main.nf | 25 ++++++++++++++++++++++++ workflows/snvphylnfc.nf | 18 +++++++++++++++++ 7 files changed, 165 insertions(+) create mode 100644 modules/local/bcftoolscall/main.nf create mode 100644 modules/local/bgzipfreebayesvcf/main.nf create mode 100644 modules/local/bgzipmpileupvcf/main.nf create mode 100644 modules/local/filterfreebayes/main.nf create mode 100644 modules/local/freebayesvcftobcf/main.nf create mode 100644 modules/local/mpileup/main.nf diff --git a/modules/local/bcftoolscall/main.nf b/modules/local/bcftoolscall/main.nf new file mode 100644 index 0000000..639b474 --- /dev/null +++ b/modules/local/bcftoolscall/main.nf @@ -0,0 +1,25 @@ +/* Bcftools call */ +process BCFTOOLS_CALL { + tag "${meta.id}" + label 'process_low' + container = "staphb/bcftools:1.15" + + input: + tuple val(meta), path(mpileup_vcf_gz) + + output: + tuple val(meta), path("${meta.id}_mpileup.bcf"), emit: mpileup_bcf + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + bcftools index -f ${mpileup_vcf_gz} + bcftools call --ploidy 1 --threads 4 --output ${prefix}_mpileup.bcf --output-type b --consensus-caller ${mpileup_vcf_gz} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/bgzipfreebayesvcf/main.nf b/modules/local/bgzipfreebayesvcf/main.nf new file mode 100644 index 0000000..7e3c850 --- /dev/null +++ b/modules/local/bgzipfreebayesvcf/main.nf @@ -0,0 +1,23 @@ +/* Zip freebayes vcf*/ +process BGZIP_FREEBAYES_VCF { + tag "$meta.id" + label 'process_low' + container = "staphb/htslib:1.15" + + input: + tuple val(meta), path(freebayes_filtered_vcf) + + output: + tuple val(meta), path("${meta.id}_freebayes_filtered.vcf.gz"), emit: filtered_zipped_vcf + path("versions.yml"), emit: versions + + script: + """ + bgzip -f ${freebayes_filtered_vcf} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bgzip (htslib): \$( bgzip --version | head --lines 1 | sed 's/bgzip (htslib) //' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/bgzipmpileupvcf/main.nf b/modules/local/bgzipmpileupvcf/main.nf new file mode 100644 index 0000000..690437a --- /dev/null +++ b/modules/local/bgzipmpileupvcf/main.nf @@ -0,0 +1,23 @@ +/* Zip mpileup vcf*/ +process BGZIP_MPILEUP_VCF { + tag "${meta.id}" + label 'process_low' + container = "staphb/htslib:1.15" + + input: + tuple val(meta), path(mpileup_vcf) + + output: + tuple val(meta), path("${meta.id}_mpileup.vcf.gz"), emit: mpileup_zipped + path("versions.yml"), emit: versions + + script: + """ + bgzip -f ${mpileup_vcf} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bgzip (htslib): \$( bgzip --version | head --lines 1 | sed 's/bgzip (htslib) //' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/filterfreebayes/main.nf b/modules/local/filterfreebayes/main.nf new file mode 100644 index 0000000..6fa93af --- /dev/null +++ b/modules/local/filterfreebayes/main.nf @@ -0,0 +1,25 @@ +/* Filter freebayes vcf */ +process FILTER_FREEBAYES { + tag "${meta.id}" + label 'process_low' + container = "staphb/snvphyl-tools:1.8.2" + + input: + tuple val(meta), path(freebayes_vcf) + + output: + tuple val(meta), path( "${meta.id}_freebayes_filtered.vcf" ), emit: filtered_vcf + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + filterVcf.pl --noindels ${freebayes_vcf} -o ${prefix}_freebayes_filtered.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + snvphyl-tools: + perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/freebayesvcftobcf/main.nf b/modules/local/freebayesvcftobcf/main.nf new file mode 100644 index 0000000..9b49b42 --- /dev/null +++ b/modules/local/freebayesvcftobcf/main.nf @@ -0,0 +1,26 @@ +/* Filtered freebayes vcf to bcf */ +process FREEBAYES_VCF_TO_BCF { + tag "${meta.id}" + label 'process_low' + container = "staphb/bcftools:1.15" + + input: + tuple val(meta), path(freebayes_filtered_vcf_gz) + + output: + tuple val(meta), path( "${meta.id}_freebayes_filtered.bcf" ), path( "${meta.id}_freebayes_filtered.bcf.csi" ), emit: filtered_bcf + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + bcftools index -f ${freebayes_filtered_vcf_gz} + bcftools view --output-type b --output-file ${prefix}_freebayes_filtered.bcf ${freebayes_filtered_vcf_gz} + bcftools index -f ${prefix}_freebayes_filtered.bcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/mpileup/main.nf b/modules/local/mpileup/main.nf new file mode 100644 index 0000000..22d019e --- /dev/null +++ b/modules/local/mpileup/main.nf @@ -0,0 +1,25 @@ +/* Mpileup */ +process MPILEUP { + tag "${meta.id}" + label 'process_low' + container = "staphb/bcftools:1.14" + + input: + tuple val(meta), path(sorted_bams) + path(refgenome) + + output: + tuple val(meta), path( "${meta.id}_mpileup.vcf" ), emit: mpileup + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + bcftools mpileup --threads 4 --fasta-ref ${refgenome} -A -B -C 0 -d 1024 -q 0 -Q 0 --output-type v -I --output ${prefix}_mpileup.vcf ${sorted_bams} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index a8a9965..aa382c0 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -45,6 +45,9 @@ include { FREEBAYES } from '../modules/local/freebayes/main' include { FILTER_FREEBAYES } from '../modules/local/filterfreebayes/main' include { BGZIP_FREEBAYES_VCF } from '../modules/local/bgzipfreebayesvcf/main' include { FREEBAYES_VCF_TO_BCF } from '../modules/local/freebayesvcftobcf/main' +include { MPILEUP } from '../modules/local/mpileup/main' +include { BGZIP_MPILEUP_VCF } from '../modules/local/bgzipmpileupvcf/main' +include { BCFTOOLS_CALL } from '../modules/local/bcftoolscall/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -127,6 +130,21 @@ workflow SNVPHYL { ) ch_versions = ch_versions.mix(FREEBAYES_VCF_TO_BCF.out.versions) + MPILEUP( + SORT_INDEX_BAMS.out.sorted_bams_and_sampleID, params.refgenome + ) + ch_versions = ch_versions.mix(MPILEUP.out.versions) + + BGZIP_MPILEUP_VCF( + MPILEUP.out.mpileup + ) + ch_versions = ch_versions.mix(BGZIP_MPILEUP_VCF.out.versions) + + BCFTOOLS_CALL( + BGZIP_MPILEUP_VCF.out.mpileup_zipped + ) + ch_versions = ch_versions.mix(BCFTOOLS_CALL.out.versions) + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From 15c08a4a7db38e52fd7fd7f405ca9d898cf80b3f Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 11 Dec 2023 15:32:24 -0600 Subject: [PATCH 007/113] added remaining modules --- bin/catWrapper.py | 37 +++++++++++++ modules/local/consolidatebcfs/main.nf | 29 ++++++++++ .../local/consolidatefiltereddensity/main.nf | 25 +++++++++ modules/local/filterstats/main.nf | 23 ++++++++ modules/local/generateline2/main.nf | 22 ++++++++ modules/local/makesnv/main.nf | 23 ++++++++ modules/local/phyml/main.nf | 26 +++++++++ modules/local/vcf2snvalignment/main.nf | 40 ++++++++++++++ nextflow.config | 2 + nextflow_schema.json | 8 +++ workflows/snvphylnfc.nf | 54 +++++++++++++++++++ 11 files changed, 289 insertions(+) create mode 100755 bin/catWrapper.py create mode 100644 modules/local/consolidatebcfs/main.nf create mode 100644 modules/local/consolidatefiltereddensity/main.nf create mode 100644 modules/local/filterstats/main.nf create mode 100644 modules/local/generateline2/main.nf create mode 100644 modules/local/makesnv/main.nf create mode 100644 modules/local/phyml/main.nf create mode 100644 modules/local/vcf2snvalignment/main.nf diff --git a/bin/catWrapper.py b/bin/catWrapper.py new file mode 100755 index 0000000..f9f88ae --- /dev/null +++ b/bin/catWrapper.py @@ -0,0 +1,37 @@ +#!/usr/bin/env python +#By, Guruprasad Ananda. + +import sys, os + +def stop_err(msg): + sys.stderr.write(msg) + sys.exit() + +def main(): + outfile = sys.argv[1] + infile = sys.argv[2] + + try: + fout = open(sys.argv[1],'w') + except: + stop_err("Output file cannot be opened for writing.") + + try: + fin = open(sys.argv[2],'r') + except: + stop_err("Input file cannot be opened for reading.") + + if len(sys.argv) < 4: + os.system("cp %s %s" %(infile,outfile)) + sys.exit() + + cmdline = "cat %s " %(infile) + for inp in sys.argv[3:]: + cmdline = cmdline + inp + " " + cmdline = cmdline + ">" + outfile + try: + os.system(cmdline) + except: + stop_err("Error encountered with cat.") + +if __name__ == "__main__": main() \ No newline at end of file diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf new file mode 100644 index 0000000..a2571f3 --- /dev/null +++ b/modules/local/consolidatebcfs/main.nf @@ -0,0 +1,29 @@ +/* Consolidate bcfs */ +process CONSOLIDATE_BCFS { + tag "${meta.id}" + label 'process_single' + container = "staphb/snvphyl-tools:1.8.2" + + input: + tuple val(meta), path(mpileup_bcf), path(freebayes_filtered_bcf), path(freebayes_filtered_csi) + + output: + path( "${meta.id}_consolidated.bcf" ), emit: consolidated_bcfs + path( "${meta.id}_consolidated.vcf" ), emit: consolidated_vcfs + path( "${meta.id}_consolidated.bcf.csi" ), emit: consolidated_bcf_index + path( "${meta.id}_filtered_density.txt" ), emit: filtered_densities + path("versions.yml"), emit: versions + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + consolidate_vcfs.pl --coverage-cutoff 10 --min-mean-mapping 30 --snv-abundance-ratio 0.75 --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf + bcftools index -f ${prefix}_consolidated.bcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + snvphyl-tools: + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/consolidatefiltereddensity/main.nf b/modules/local/consolidatefiltereddensity/main.nf new file mode 100644 index 0000000..cf587ad --- /dev/null +++ b/modules/local/consolidatefiltereddensity/main.nf @@ -0,0 +1,25 @@ +/* CONSOLIDATED_ALL */ +process CONSOLIDATE_FILTERED_DENSITY { + label 'process_low' + container = 'quay.io/jvhagey/phoenix:base_v1.0.0' + + input: + path(filtered_densities) + path(invalid_positions) + + output: + path('filtered_density_all.txt'), emit: filtered_densities + path('new_invalid_positions.bed'), emit: new_invalid_positions + path("versions.yml"), emit: versions + + script: + """ + find ./ -name '*_filtered_density.txt' -exec cat {} + > filtered_density_all.txt + catWrapper.py new_invalid_positions.bed filtered_density_all.txt ${invalid_positions} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/filterstats/main.nf b/modules/local/filterstats/main.nf new file mode 100644 index 0000000..23b0c71 --- /dev/null +++ b/modules/local/filterstats/main.nf @@ -0,0 +1,23 @@ +/* Filter Stats */ +process FILTER_STATS { + label 'process_low' + container = "staphb/snvphyl-tools:1.8.2" + + input: + path(snvTable) + + output: + path('filterStats.txt'), emit: filter_stats + path("versions.yml"), emit: versions + + script: + """ + filter-stats.pl -i ${snvTable} -a > filterStats.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + snvphyl-tools: + perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/generateline2/main.nf b/modules/local/generateline2/main.nf new file mode 100644 index 0000000..5a4955f --- /dev/null +++ b/modules/local/generateline2/main.nf @@ -0,0 +1,22 @@ +/* Generate a line for the next process */ +process GENERATE_LINE_2 { + label 'process_low' + container 'quay.io/jvhagey/phoenix:base_v1.0.0' + + input: + path(consolidated_bcf) + + output: + path("consolidation_line.txt"), emit: consolidation_line + //path("versions.yml"), emit: versions + + shell: + ''' + #! /bin/bash -l + for f in *_consolidated.bcf + do + fname=\$(basename $f _consolidated.bcf) + echo "--consolidate_vcf \$fname=$f " | tr -d "\n" >> consolidation_line.txt + done + ''' +} \ No newline at end of file diff --git a/modules/local/makesnv/main.nf b/modules/local/makesnv/main.nf new file mode 100644 index 0000000..61cbbac --- /dev/null +++ b/modules/local/makesnv/main.nf @@ -0,0 +1,23 @@ +/* Making SNV matrix */ +process MAKE_SNV { + label 'process_low' + container = "staphb/snvphyl-tools:1.8.2" + + input: + path(snvAlignment_phy) + + output: + path('snvMatrix.tsv'), emit: snvMatrix + path("versions.yml"), emit: versions + + script: + """ + snv_matrix.pl ${snvAlignment_phy} -o snvMatrix.tsv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + snvphyl-tools: + perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/modules/local/phyml/main.nf b/modules/local/phyml/main.nf new file mode 100644 index 0000000..486530b --- /dev/null +++ b/modules/local/phyml/main.nf @@ -0,0 +1,26 @@ +/* PHYML to make tree */ +process PHYML { + label 'process_low' + //container = "staphb/phyml:3.3.20220408" + container = "https://depot.galaxyproject.org/singularity/phyml:3.3.20211231--hee9e358_0" + + input: + path(snvAlignment_phy) + + output: + path('phylogeneticTree.newick'), emit: phylogeneticTree + path('phylogeneticTreeStats.txt'), emit: phylogeneticTreeStats + path("versions.yml"), emit: versions + + script: + """ + phyml -i ${snvAlignment_phy} --datatype nt --model GTR -v 0.0 --ts/tv e --nclasses 4 --alpha e --bootstrap -4 --quiet + mv snvAlignment.phy_phyml_stats.txt phylogeneticTreeStats.txt + mv snvAlignment.phy_phyml_tree.txt phylogeneticTree.newick + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + phyml: \$( phyml --version | grep -P -o '[0-9]+.[0-9]+.[0-9]+' ) + END_VERSIONS + """ +} diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf new file mode 100644 index 0000000..89a79d4 --- /dev/null +++ b/modules/local/vcf2snvalignment/main.nf @@ -0,0 +1,40 @@ +/* VCF2SNV_ALIGNMENT Call variants */ +def VERSION = '1.8.2' // Version information not provided by tool on CLI + +process VCF2SNV_ALIGNMENT { + label 'process_single' + container = "staphb/snvphyl-tools:1.8.2" + + input: + val(consolidate_bcfs) + path(bcf) + path(new_invalid_positions) + path(refgenome) + path(consolidated_bcf_index) + + output: + path('snvAlignment.phy'), emit: snvAlignment + path('vcf2core.tsv'), emit: vcf2core + path('snvTable.tsv'), emit: snvTable + path("versions.yml"), emit: versions + + script: + """ + vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus 4 --output-base snvalign --fasta ${refgenome} ${consolidate_bcfs} + mv snvalign-positions.tsv snvTable.tsv + mv snvalign-stats.csv vcf2core.tsv + if [[ -f snvalign.phy ]]; then + mv snvalign.phy snvAlignment.phy + sed -i "s/'//" snvAlignment.phy + sed -i "s/'//" snvAlignment.phy + else + touch snvAlignment.phy + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + snvphyl-tools: + perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) + END_VERSIONS + """ +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 05cfc38..206bb08 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,6 +12,8 @@ params { // Input options input = null refgenome = "${baseDir}/assets/reference.fasta" + window_size = 11 + density_threshold = 2 // Boilerplate options outdir = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 493c591..ac5190b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -210,6 +210,14 @@ "refgenome": { "type": "string", "default": "/home/eric/projects/snvphylnfc/assets/reference.fasta" + }, + "window_size": { + "type": "integer", + "default": 11 + }, + "density_threshold": { + "type": "integer", + "default": 2 } } } diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index aa382c0..394f4fb 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -48,6 +48,13 @@ include { FREEBAYES_VCF_TO_BCF } from '../modules/local/freebayesvcftobcf/main' include { MPILEUP } from '../modules/local/mpileup/main' include { BGZIP_MPILEUP_VCF } from '../modules/local/bgzipmpileupvcf/main' include { BCFTOOLS_CALL } from '../modules/local/bcftoolscall/main' +include { CONSOLIDATE_BCFS } from '../modules/local/consolidatebcfs/main' +include { CONSOLIDATE_FILTERED_DENSITY } from '../modules/local/consolidatefiltereddensity/main' +include { GENERATE_LINE_2 } from '../modules/local/generateline2/main' +include { VCF2SNV_ALIGNMENT } from '../modules/local/vcf2snvalignment/main' +include { FILTER_STATS } from '../modules/local/filterstats/main' +include { PHYML } from '../modules/local/phyml/main' +include { MAKE_SNV } from '../modules/local/makesnv/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -145,6 +152,53 @@ workflow SNVPHYL { ) ch_versions = ch_versions.mix(BCFTOOLS_CALL.out.versions) + //Joining channels of multiple outputs + combined_ch = BCFTOOLS_CALL.out.mpileup_bcf.join(FREEBAYES_VCF_TO_BCF.out.filtered_bcf) + //11. consolidate variant calling files process takes 2 input channels as arguments + CONSOLIDATE_BCFS( + combined_ch + ) + ch_versions = ch_versions.mix(CONSOLIDATE_BCFS.out.versions) + + // Concat filtered densities to make new invalid_postions + CONSOLIDATE_FILTERED_DENSITY( + CONSOLIDATE_BCFS.out.filtered_densities.collect(), FIND_REPEATS.out.repeats_bed_file + ) + ch_versions = ch_versions.mix(CONSOLIDATE_FILTERED_DENSITY.out.versions) + + // Making string that looks like... this is needed for the next process + //--consolidate_vcf 2021JQ-00457-WAPHL-M5130-211029=2021JQ-00457-WAPHL-M5130-211029_consolidated.bcf --consolidate_vcf 2021JQ-00459-WAPHL-M5130-211029=2021JQ-00459-WAPHL-M5130-211029_consolidated.bcf --consolidate_vcf 2021JQ-00460-WAPHL-M5130-211029=2021JQ-00460-WAPHL-M5130-211029_consolidated.bcf + GENERATE_LINE_2( + CONSOLIDATE_BCFS.out.consolidated_bcfs.collect() + ) + //ch_versions = ch_versions.mix(GENERATE_LINE_2.out.versions) + + // Get line out of file we just made that has the --consolidate_vcf line... + //13. consolidate variant calling files process takes 2 input channels as arguments + VCF2SNV_ALIGNMENT( + GENERATE_LINE_2.out.consolidation_line.splitText(), CONSOLIDATE_BCFS.out.consolidated_bcfs.collect(), CONSOLIDATE_FILTERED_DENSITY.out.new_invalid_positions, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() + ) + ch_versions = ch_versions.mix(VCF2SNV_ALIGNMENT.out.versions) + + //14. Filter Stats + FILTER_STATS( + VCF2SNV_ALIGNMENT.out.snvTable + ) + ch_versions = ch_versions.mix(FILTER_STATS.out.versions) + + //15. Using phyml to build tree process takes 1 input channel as an argument + PHYML( + VCF2SNV_ALIGNMENT.out.snvAlignment + ) + ch_versions = ch_versions.mix(PHYML.out.versions) + + //16. Make SNVMatix.tsv + MAKE_SNV( + VCF2SNV_ALIGNMENT.out.snvAlignment + ) + ch_versions = ch_versions.mix(MAKE_SNV.out.versions) + + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From 9c6266e822852cd372dbff247628bb15b7dfb90c Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 13 Dec 2023 11:51:27 -0600 Subject: [PATCH 008/113] Changing example data. --- assets/reference.fasta | 11 +++++++++++ assets/samplesheet.csv | 6 +++--- 2 files changed, 14 insertions(+), 3 deletions(-) create mode 100644 assets/reference.fasta diff --git a/assets/reference.fasta b/assets/reference.fasta new file mode 100644 index 0000000..44aea1f --- /dev/null +++ b/assets/reference.fasta @@ -0,0 +1,11 @@ +>reference +TCCACAAGCCATTGTGTGTAATTAACCACTAATTGTGTATAAGTTTAAACTAATTGAAAAGGTTATCCAC +AATAAAAAGGCGTTATTCAGGAGTTATCCACACTTTCTAGGAAAGGATTTCATTGCGCCAATGTGTTAAA +CTATTTACCGAATACGAAAAAAAGACAAATAAATGAGGTTGTGAAAAATGATATTTCAACGGCTTTTGAA +AACTAGAGATACAGAGTTTTATCGAGTTATACAAAACAGGAATATTGACGACGTATTTGGATACTTATTA +ATTCACGATAAACGGGAACCAGCAGAAATTGACGATTTTAAGGTATTTGCAAAAAGTAATATAAATAAAG +AAGCTTTTTCAGTGAATATCAAAAAAAATCATATTTACACGATGTTTTTCCACTTTACTGATTTAGAGGA +AGAACAGGAAATTCCAAAATTTACTAAAGTTATTCGTTTTATAGAAGGACTTTTATCTTTTCAGCCAGAA +ACAAGCCATTACGTTGATAACTATTTAATAAAGGAAAAACTAATTTTTGAATATCCTGCTGAATTTGAGA +AAATCGGGGAGTTTGCTAAATATTTAGTAAAGCTTTCGGGTCGTAAAATTACTATTCCAGACACAACGAG +AGAAAAATATATCTATTTAACGCAATAATTTTCGAAAAATGGTTTTTCTCTCTATAAAAATATGATATGA diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 814a27d..fd365a5 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,4 +1,4 @@ sample,fastq_1,fastq_2 -SAMPLE1,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample1_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample1_R2.fastq.gz -SAMPLE2,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample2_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample2_R2.fastq.gz -SAMPLE3,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample1_R1.fastq.gz, +SAMPLE1,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_2.fastq +SAMPLE2,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_2.fastq +SAMPLE3,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_2.fastq From 906cc613350f23c3b7596eb2dbba7c1c9ec610a5 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 14 Dec 2023 10:51:20 -0600 Subject: [PATCH 009/113] Switching bash-only modules from phoenix container to python. --- modules/local/generateline1/main.nf | 2 +- modules/local/generateline2/main.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/generateline1/main.nf b/modules/local/generateline1/main.nf index 495bdd5..3ed5f65 100644 --- a/modules/local/generateline1/main.nf +++ b/modules/local/generateline1/main.nf @@ -1,7 +1,7 @@ /* Generate a line for the next process */ process GENERATE_LINE_1 { label 'process_low' - container 'quay.io/jvhagey/phoenix:base_v1.0.0' + container 'docker.io/python:3.9.17' input: path(sorted_bams) diff --git a/modules/local/generateline2/main.nf b/modules/local/generateline2/main.nf index 5a4955f..af7b081 100644 --- a/modules/local/generateline2/main.nf +++ b/modules/local/generateline2/main.nf @@ -1,7 +1,7 @@ /* Generate a line for the next process */ process GENERATE_LINE_2 { label 'process_low' - container 'quay.io/jvhagey/phoenix:base_v1.0.0' + container 'docker.io/python:3.9.17' input: path(consolidated_bcf) From 9a2b9564e68c45e5ff823439fd8e4a88803237bf Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 14 Dec 2023 11:44:44 -0600 Subject: [PATCH 010/113] Adding legal requirements for including Apache v2 work. --- LICENSE-Apachev2.txt | 202 ++++++++++++++++++ README.md | 12 +- modules/local/bcftoolscall/main.nf | 10 +- modules/local/bgzipfreebayesvcf/main.nf | 10 +- modules/local/bgzipmpileupvcf/main.nf | 10 +- modules/local/consolidatebcfs/main.nf | 10 +- .../local/consolidatefiltereddensity/main.nf | 10 +- modules/local/filterfreebayes/main.nf | 10 +- modules/local/filterstats/main.nf | 10 +- modules/local/findrepeats/main.nf | 10 +- modules/local/freebayes/main.nf | 10 +- modules/local/freebayesvcftobcf/main.nf | 10 +- modules/local/generateline1/main.nf | 10 +- modules/local/generateline2/main.nf | 10 +- modules/local/indexing/main.nf | 9 + modules/local/makesnv/main.nf | 10 +- modules/local/mpileup/main.nf | 10 +- modules/local/phyml/main.nf | 10 +- modules/local/smaltmap/main.nf | 10 +- modules/local/sortindexbams/main.nf | 10 +- modules/local/vcf2snvalignment/main.nf | 10 +- modules/local/verifyingmapq/main.nf | 10 +- 22 files changed, 393 insertions(+), 20 deletions(-) create mode 100644 LICENSE-Apachev2.txt diff --git a/LICENSE-Apachev2.txt b/LICENSE-Apachev2.txt new file mode 100644 index 0000000..57bc88a --- /dev/null +++ b/LICENSE-Apachev2.txt @@ -0,0 +1,202 @@ + Apache License + Version 2.0, January 2004 + http://www.apache.org/licenses/ + + TERMS AND CONDITIONS FOR USE, REPRODUCTION, AND DISTRIBUTION + + 1. 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We also recommend that a + file or class name and description of purpose be included on the + same "printed page" as the copyright notice for easier + identification within third-party archives. + + Copyright [yyyy] [name of copyright owner] + + Licensed under the Apache License, Version 2.0 (the "License"); + you may not use this file except in compliance with the License. + You may obtain a copy of the License at + + http://www.apache.org/licenses/LICENSE-2.0 + + Unless required by applicable law or agreed to in writing, software + distributed under the License is distributed on an "AS IS" BASIS, + WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. + See the License for the specific language governing permissions and + limitations under the License. + diff --git a/README.md b/README.md index c6198f5..302732c 100644 --- a/README.md +++ b/README.md @@ -4,10 +4,12 @@ This is the in-development nf-core-based pipeline for SNVPhyl. -This pipeline is adapted from a Nextflow pipeline for SNVPhyl developed by [Jill Hagey](https://github.com/DHQP/SNVPhyl_Nextflow). + # Legal +## SNVPhyl NF-Core Pipeline + Copyright 2023 Government of Canada Licensed under the MIT License (the "License"); you may not use @@ -20,3 +22,11 @@ Unless required by applicable law or agreed to in writing, software distributed under the License is distributed on an "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License. + +## Derivative Work + +This pipeline includes source code from a [nf-core pipeline for SNVPhyl](https://github.com/DHQP/SNVPhyl_Nextflow) developed by Jill Hagey as a work of the United States Government that was not subject to domestic copyright protection under 17 USC ยง 105. This work by the United States Government is in the public domain within the United States, and copyright and related rights for the work worldwide are waived through the CC0 1.0 Universal public domain dedication. + +The included source code developed by Jill Hagey as a work of the United States Government was distributed under the Apache Software License version 2. A copy of the Apache Software License is [included in this repository](LICENSE-Apachev2.txt). + +Any such source files in this project that are included from or derived from the original work by Jill Hagey will include a notice. diff --git a/modules/local/bcftoolscall/main.nf b/modules/local/bcftoolscall/main.nf index 639b474..40ad3de 100644 --- a/modules/local/bcftoolscall/main.nf +++ b/modules/local/bcftoolscall/main.nf @@ -1,4 +1,12 @@ -/* Bcftools call */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process BCFTOOLS_CALL { tag "${meta.id}" label 'process_low' diff --git a/modules/local/bgzipfreebayesvcf/main.nf b/modules/local/bgzipfreebayesvcf/main.nf index 7e3c850..53c193f 100644 --- a/modules/local/bgzipfreebayesvcf/main.nf +++ b/modules/local/bgzipfreebayesvcf/main.nf @@ -1,4 +1,12 @@ -/* Zip freebayes vcf*/ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process BGZIP_FREEBAYES_VCF { tag "$meta.id" label 'process_low' diff --git a/modules/local/bgzipmpileupvcf/main.nf b/modules/local/bgzipmpileupvcf/main.nf index 690437a..0f10ca0 100644 --- a/modules/local/bgzipmpileupvcf/main.nf +++ b/modules/local/bgzipmpileupvcf/main.nf @@ -1,4 +1,12 @@ -/* Zip mpileup vcf*/ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process BGZIP_MPILEUP_VCF { tag "${meta.id}" label 'process_low' diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index a2571f3..5fb13ea 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -1,4 +1,12 @@ -/* Consolidate bcfs */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process CONSOLIDATE_BCFS { tag "${meta.id}" label 'process_single' diff --git a/modules/local/consolidatefiltereddensity/main.nf b/modules/local/consolidatefiltereddensity/main.nf index cf587ad..5703b31 100644 --- a/modules/local/consolidatefiltereddensity/main.nf +++ b/modules/local/consolidatefiltereddensity/main.nf @@ -1,4 +1,12 @@ -/* CONSOLIDATED_ALL */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process CONSOLIDATE_FILTERED_DENSITY { label 'process_low' container = 'quay.io/jvhagey/phoenix:base_v1.0.0' diff --git a/modules/local/filterfreebayes/main.nf b/modules/local/filterfreebayes/main.nf index 6fa93af..0358a2e 100644 --- a/modules/local/filterfreebayes/main.nf +++ b/modules/local/filterfreebayes/main.nf @@ -1,4 +1,12 @@ -/* Filter freebayes vcf */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process FILTER_FREEBAYES { tag "${meta.id}" label 'process_low' diff --git a/modules/local/filterstats/main.nf b/modules/local/filterstats/main.nf index 23b0c71..7547ba6 100644 --- a/modules/local/filterstats/main.nf +++ b/modules/local/filterstats/main.nf @@ -1,4 +1,12 @@ -/* Filter Stats */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process FILTER_STATS { label 'process_low' container = "staphb/snvphyl-tools:1.8.2" diff --git a/modules/local/findrepeats/main.nf b/modules/local/findrepeats/main.nf index 098d642..0795be5 100644 --- a/modules/local/findrepeats/main.nf +++ b/modules/local/findrepeats/main.nf @@ -1,4 +1,12 @@ -/* Find repeats and create invaild_positions.bed */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process FIND_REPEATS { tag "${refgenome}" label 'process_low' diff --git a/modules/local/freebayes/main.nf b/modules/local/freebayes/main.nf index a4b45a6..1e023b3 100644 --- a/modules/local/freebayes/main.nf +++ b/modules/local/freebayes/main.nf @@ -1,4 +1,12 @@ -/* Freebayes variant calling */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process FREEBAYES { tag "$meta.id" label 'process_low' diff --git a/modules/local/freebayesvcftobcf/main.nf b/modules/local/freebayesvcftobcf/main.nf index 9b49b42..aba9edd 100644 --- a/modules/local/freebayesvcftobcf/main.nf +++ b/modules/local/freebayesvcftobcf/main.nf @@ -1,4 +1,12 @@ -/* Filtered freebayes vcf to bcf */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process FREEBAYES_VCF_TO_BCF { tag "${meta.id}" label 'process_low' diff --git a/modules/local/generateline1/main.nf b/modules/local/generateline1/main.nf index 3ed5f65..13faa8b 100644 --- a/modules/local/generateline1/main.nf +++ b/modules/local/generateline1/main.nf @@ -1,4 +1,12 @@ -/* Generate a line for the next process */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process GENERATE_LINE_1 { label 'process_low' container 'docker.io/python:3.9.17' diff --git a/modules/local/generateline2/main.nf b/modules/local/generateline2/main.nf index af7b081..ea058d6 100644 --- a/modules/local/generateline2/main.nf +++ b/modules/local/generateline2/main.nf @@ -1,4 +1,12 @@ -/* Generate a line for the next process */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process GENERATE_LINE_2 { label 'process_low' container 'docker.io/python:3.9.17' diff --git a/modules/local/indexing/main.nf b/modules/local/indexing/main.nf index cb7d368..d1c78c2 100644 --- a/modules/local/indexing/main.nf +++ b/modules/local/indexing/main.nf @@ -1,3 +1,12 @@ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process INDEXING { tag "${refgenome}" label 'process_low' diff --git a/modules/local/makesnv/main.nf b/modules/local/makesnv/main.nf index 61cbbac..8e2f89a 100644 --- a/modules/local/makesnv/main.nf +++ b/modules/local/makesnv/main.nf @@ -1,4 +1,12 @@ -/* Making SNV matrix */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process MAKE_SNV { label 'process_low' container = "staphb/snvphyl-tools:1.8.2" diff --git a/modules/local/mpileup/main.nf b/modules/local/mpileup/main.nf index 22d019e..09ef5de 100644 --- a/modules/local/mpileup/main.nf +++ b/modules/local/mpileup/main.nf @@ -1,4 +1,12 @@ -/* Mpileup */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process MPILEUP { tag "${meta.id}" label 'process_low' diff --git a/modules/local/phyml/main.nf b/modules/local/phyml/main.nf index 486530b..831c53c 100644 --- a/modules/local/phyml/main.nf +++ b/modules/local/phyml/main.nf @@ -1,4 +1,12 @@ -/* PHYML to make tree */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process PHYML { label 'process_low' //container = "staphb/phyml:3.3.20220408" diff --git a/modules/local/smaltmap/main.nf b/modules/local/smaltmap/main.nf index 16b0b4b..964d72e 100644 --- a/modules/local/smaltmap/main.nf +++ b/modules/local/smaltmap/main.nf @@ -1,4 +1,12 @@ -/* Map reads to reference genome & create BAM file */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process SMALT_MAP { tag "$meta.id" label 'process_single' diff --git a/modules/local/sortindexbams/main.nf b/modules/local/sortindexbams/main.nf index 315ab58..9ac8740 100644 --- a/modules/local/sortindexbams/main.nf +++ b/modules/local/sortindexbams/main.nf @@ -1,4 +1,12 @@ -/* Create BAMs and Sort */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process SORT_INDEX_BAMS { tag "$meta.id" label 'process_low' diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index 89a79d4..cbf89dc 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -1,4 +1,12 @@ -/* VCF2SNV_ALIGNMENT Call variants */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ def VERSION = '1.8.2' // Version information not provided by tool on CLI process VCF2SNV_ALIGNMENT { diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index c5e83ec..eafe83c 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -1,4 +1,12 @@ -/* Verifying Mapping Quality */ +/* +This source file is adapted from a nf-core pipeline for SNVPhyl developed by +Jill Hagey as a work of the United States Government, which was distributed as +a work within the public domain under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +*/ process VERIFYING_MAP_Q { tag "" label 'process_single' From 1ec25ca9893b26e9ae70c2e6850f7a96131233cd Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 14 Dec 2023 11:56:38 -0600 Subject: [PATCH 011/113] Attempting to correctly attribute this Python file. --- bin/catWrapper.py | 11 ++++++++++- 1 file changed, 10 insertions(+), 1 deletion(-) diff --git a/bin/catWrapper.py b/bin/catWrapper.py index f9f88ae..3e06f69 100755 --- a/bin/catWrapper.py +++ b/bin/catWrapper.py @@ -1,5 +1,14 @@ #!/usr/bin/env python -#By, Guruprasad Ananda. +""" +This source file was developed by Guruprasad Ananda and was included in a +nf-core pipeline for SNVPhyl developed by Jill Hagey as a work of the United +States Government, which was distributed as a work within the public domain +under the Apache Software License version 2. + +This source file has been adapted to work within our pipeline. + +Please refer to the README for more information. +""" import sys, os From 14ce63545518933331b58ff8e683a061903c5a6c Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 14 Dec 2023 11:59:57 -0600 Subject: [PATCH 012/113] Attributing the main workflow. --- workflows/snvphylnfc.nf | 12 ++++++++++++ 1 file changed, 12 insertions(+) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 394f4fb..8401668 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -1,3 +1,15 @@ +/* +This source file includes source from a nf-core pipeline for SNVPhyl developed +by Jill Hagey as a work of the United States Government, which was distributed +as a work within the public domain under the Apache Software License version 2. + +The work by Jill Hagey has been adapted such that we utilize the modules and +module calls in the original source work, but make changes such the pipeline +functions according to our desired specifications and outcomes. + +Please refer to the README for more information. +*/ + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ PRINT PARAMS SUMMARY From 43c1f90d2b2803eea1ef71852c138d6ba796d987 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 15 Dec 2023 10:46:46 -0600 Subject: [PATCH 013/113] Trying to support single end reads. --- modules/local/smaltmap/main.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/modules/local/smaltmap/main.nf b/modules/local/smaltmap/main.nf index 964d72e..abb3da2 100644 --- a/modules/local/smaltmap/main.nf +++ b/modules/local/smaltmap/main.nf @@ -24,9 +24,10 @@ process SMALT_MAP { script: def prefix = task.ext.prefix ?: "${meta.id}" + def smalt_map_command = reads[1] ? "smalt map -f bam -n 4 -l pe -i 1000 -j 20 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]} ${reads[1]}": "smalt map -f bam -n 4 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]}" """ REFNAME=\$(basename ${ref_sma} .sma) - smalt map -f bam -n 4 -l pe -i 1000 -j 20 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]} ${reads[1]} + ${smalt_map_command} smalt version cat <<-END_VERSIONS > versions.yml From 4dafaa8bbf9e313a70fb097e7ddd0aef00434650 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 15 Dec 2023 15:30:25 -0600 Subject: [PATCH 014/113] Single end, minimum coverage 5x. --- modules/local/consolidatebcfs/main.nf | 2 +- modules/local/verifyingmapq/main.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index 5fb13ea..7e2557a 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -25,7 +25,7 @@ process CONSOLIDATE_BCFS { script: def prefix = task.ext.prefix ?: "${meta.id}" """ - consolidate_vcfs.pl --coverage-cutoff 10 --min-mean-mapping 30 --snv-abundance-ratio 0.75 --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf + consolidate_vcfs.pl --coverage-cutoff 5 --min-mean-mapping 30 --snv-abundance-ratio 0.75 --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf bcftools index -f ${prefix}_consolidated.bcf cat <<-END_VERSIONS > versions.yml diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index eafe83c..6a51df2 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -22,7 +22,7 @@ process VERIFYING_MAP_Q { script: """ - verify_mapping_quality.pl -c 4 --min-depth 10 --min-map 80 --output mappingQuality.txt ${bam_line} + verify_mapping_quality.pl -c 4 --min-depth 5 --min-map 80 --output mappingQuality.txt ${bam_line} cat <<-END_VERSIONS > versions.yml "${task.process}": From e0fdae9d5469f1b5af661b3a8db5a499342856e9 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 15 Dec 2023 15:31:15 -0600 Subject: [PATCH 015/113] Switching from Phoenix container to Python. --- modules/local/consolidatefiltereddensity/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/consolidatefiltereddensity/main.nf b/modules/local/consolidatefiltereddensity/main.nf index 5703b31..81c4e12 100644 --- a/modules/local/consolidatefiltereddensity/main.nf +++ b/modules/local/consolidatefiltereddensity/main.nf @@ -9,7 +9,7 @@ Please refer to the README for more information. */ process CONSOLIDATE_FILTERED_DENSITY { label 'process_low' - container = 'quay.io/jvhagey/phoenix:base_v1.0.0' + container = 'docker.io/python:3.9.17' input: path(filtered_densities) From b0b9df902f0cb9878c5aeaa07804d3f26ebbea41 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 09:26:53 -0600 Subject: [PATCH 016/113] Formatting, schema, linting. --- README.md | 2 -- bin/catWrapper.py | 26 ++++++++++++++----------- nextflow_schema.json | 45 +++++++++++++++++++++++++++++--------------- 3 files changed, 45 insertions(+), 28 deletions(-) diff --git a/README.md b/README.md index 302732c..ca2f74c 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,6 @@ This is the in-development nf-core-based pipeline for SNVPhyl. - - # Legal ## SNVPhyl NF-Core Pipeline diff --git a/bin/catWrapper.py b/bin/catWrapper.py index 3e06f69..3274c1f 100755 --- a/bin/catWrapper.py +++ b/bin/catWrapper.py @@ -12,29 +12,31 @@ import sys, os + def stop_err(msg): sys.stderr.write(msg) sys.exit() - + + def main(): outfile = sys.argv[1] infile = sys.argv[2] - + try: - fout = open(sys.argv[1],'w') + fout = open(sys.argv[1], "w") except: stop_err("Output file cannot be opened for writing.") - + try: - fin = open(sys.argv[2],'r') + fin = open(sys.argv[2], "r") except: stop_err("Input file cannot be opened for reading.") - + if len(sys.argv) < 4: - os.system("cp %s %s" %(infile,outfile)) + os.system("cp %s %s" % (infile, outfile)) sys.exit() - - cmdline = "cat %s " %(infile) + + cmdline = "cat %s " % (infile) for inp in sys.argv[3:]: cmdline = cmdline + inp + " " cmdline = cmdline + ">" + outfile @@ -42,5 +44,7 @@ def main(): os.system(cmdline) except: stop_err("Error encountered with cat.") - -if __name__ == "__main__": main() \ No newline at end of file + + +if __name__ == "__main__": + main() diff --git a/nextflow_schema.json b/nextflow_schema.json index ac5190b..811d1be 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -29,6 +29,11 @@ "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", "fa_icon": "fas fa-folder-open" }, + "refgenome": { + "type": "string", + "description": "The file path to the FASTA-format reference genome to use.", + "fa_icon": "fas fa-file" + }, "email": { "type": "string", "description": "Email address for completion summary.", @@ -38,6 +43,27 @@ } } }, + "snvphyl_pipeline_parameters": { + "title": "SNVPhyl pipeline parameters", + "type": "object", + "description": "Parameters to configure specific pipeline behaviour.", + "default": "", + "properties": { + "window_size": { + "type": "integer", + "default": 11, + "description": "Used during BCF consolidation.", + "fa_icon": "fas fa-desktop" + }, + "density_threshold": { + "type": "integer", + "default": 2, + "description": "Used during BCF consolidation.", + "fa_icon": "fas fa-desktop" + } + }, + "fa_icon": "fas fa-terminal" + }, "institutional_config_options": { "title": "Institutional config options", "type": "object", @@ -196,6 +222,9 @@ { "$ref": "#/definitions/input_output_options" }, + { + "$ref": "#/definitions/snvphyl_pipeline_parameters" + }, { "$ref": "#/definitions/institutional_config_options" }, @@ -205,19 +234,5 @@ { "$ref": "#/definitions/generic_options" } - ], - "properties": { - "refgenome": { - "type": "string", - "default": "/home/eric/projects/snvphylnfc/assets/reference.fasta" - }, - "window_size": { - "type": "integer", - "default": 11 - }, - "density_threshold": { - "type": "integer", - "default": 2 - } - } + ] } From 39d597ff10165517f2fa86758a552cec499aa64e Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 09:35:25 -0600 Subject: [PATCH 017/113] Formatting --- .github/workflows/linting.yml | 2 +- modules/local/bcftoolscall/main.nf | 2 +- modules/local/bgzipfreebayesvcf/main.nf | 2 +- modules/local/bgzipmpileupvcf/main.nf | 2 +- modules/local/consolidatebcfs/main.nf | 2 +- modules/local/consolidatefiltereddensity/main.nf | 2 +- modules/local/filterfreebayes/main.nf | 2 +- modules/local/filterstats/main.nf | 2 +- modules/local/findrepeats/main.nf | 2 +- modules/local/freebayes/main.nf | 2 +- modules/local/freebayesvcftobcf/main.nf | 2 +- modules/local/generateline1/main.nf | 4 ++-- modules/local/generateline2/main.nf | 2 +- modules/local/indexing/main.nf | 2 +- modules/local/makesnv/main.nf | 2 +- modules/local/mpileup/main.nf | 2 +- modules/local/smaltmap/main.nf | 2 +- modules/local/sortindexbams/main.nf | 2 +- modules/local/vcf2snvalignment/main.nf | 12 ++++++------ modules/local/verifyingmapq/main.nf | 4 ++-- 20 files changed, 27 insertions(+), 27 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd21..9a48144 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -22,7 +22,7 @@ jobs: run: npm install -g editorconfig-checker - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') + run: editorconfig-checker -exclude README.md LICENSE-Apachev2.txt $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') Prettier: runs-on: ubuntu-latest diff --git a/modules/local/bcftoolscall/main.nf b/modules/local/bcftoolscall/main.nf index 40ad3de..2bddeb8 100644 --- a/modules/local/bcftoolscall/main.nf +++ b/modules/local/bcftoolscall/main.nf @@ -30,4 +30,4 @@ process BCFTOOLS_CALL { bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/bgzipfreebayesvcf/main.nf b/modules/local/bgzipfreebayesvcf/main.nf index 53c193f..0e3c466 100644 --- a/modules/local/bgzipfreebayesvcf/main.nf +++ b/modules/local/bgzipfreebayesvcf/main.nf @@ -28,4 +28,4 @@ process BGZIP_FREEBAYES_VCF { bgzip (htslib): \$( bgzip --version | head --lines 1 | sed 's/bgzip (htslib) //' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/bgzipmpileupvcf/main.nf b/modules/local/bgzipmpileupvcf/main.nf index 0f10ca0..589fa6f 100644 --- a/modules/local/bgzipmpileupvcf/main.nf +++ b/modules/local/bgzipmpileupvcf/main.nf @@ -28,4 +28,4 @@ process BGZIP_MPILEUP_VCF { bgzip (htslib): \$( bgzip --version | head --lines 1 | sed 's/bgzip (htslib) //' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index 7e2557a..bae7fd3 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -34,4 +34,4 @@ process CONSOLIDATE_BCFS { bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/consolidatefiltereddensity/main.nf b/modules/local/consolidatefiltereddensity/main.nf index 81c4e12..509c131 100644 --- a/modules/local/consolidatefiltereddensity/main.nf +++ b/modules/local/consolidatefiltereddensity/main.nf @@ -30,4 +30,4 @@ process CONSOLIDATE_FILTERED_DENSITY { python: \$(python --version | sed 's/Python //g') END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/filterfreebayes/main.nf b/modules/local/filterfreebayes/main.nf index 0358a2e..3ad851f 100644 --- a/modules/local/filterfreebayes/main.nf +++ b/modules/local/filterfreebayes/main.nf @@ -30,4 +30,4 @@ process FILTER_FREEBAYES { perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/filterstats/main.nf b/modules/local/filterstats/main.nf index 7547ba6..1317c43 100644 --- a/modules/local/filterstats/main.nf +++ b/modules/local/filterstats/main.nf @@ -28,4 +28,4 @@ process FILTER_STATS { perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/findrepeats/main.nf b/modules/local/findrepeats/main.nf index 0795be5..7e767ad 100644 --- a/modules/local/findrepeats/main.nf +++ b/modules/local/findrepeats/main.nf @@ -28,4 +28,4 @@ process FIND_REPEATS { snvphyl-tools: perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) """ -} \ No newline at end of file +} diff --git a/modules/local/freebayes/main.nf b/modules/local/freebayes/main.nf index 1e023b3..e37b263 100644 --- a/modules/local/freebayes/main.nf +++ b/modules/local/freebayes/main.nf @@ -30,4 +30,4 @@ process FREEBAYES { freebayes: \$(echo \$(freebayes --version 2>&1) | sed 's/version:\s*v//g' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/freebayesvcftobcf/main.nf b/modules/local/freebayesvcftobcf/main.nf index aba9edd..66458a6 100644 --- a/modules/local/freebayesvcftobcf/main.nf +++ b/modules/local/freebayesvcftobcf/main.nf @@ -31,4 +31,4 @@ process FREEBAYES_VCF_TO_BCF { bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/generateline1/main.nf b/modules/local/generateline1/main.nf index 13faa8b..d5e7bb5 100644 --- a/modules/local/generateline1/main.nf +++ b/modules/local/generateline1/main.nf @@ -21,11 +21,11 @@ process GENERATE_LINE_1 { shell: ''' #! /bin/bash -l - count=0 + count=0 for f in *_sorted.bam do ((count++)) echo "--bam bam\$count=./$f " | tr -d "\n" >> bam_line.txt done ''' -} \ No newline at end of file +} diff --git a/modules/local/generateline2/main.nf b/modules/local/generateline2/main.nf index ea058d6..8ac71af 100644 --- a/modules/local/generateline2/main.nf +++ b/modules/local/generateline2/main.nf @@ -27,4 +27,4 @@ process GENERATE_LINE_2 { echo "--consolidate_vcf \$fname=$f " | tr -d "\n" >> consolidation_line.txt done ''' -} \ No newline at end of file +} diff --git a/modules/local/indexing/main.nf b/modules/local/indexing/main.nf index d1c78c2..a8a8228 100644 --- a/modules/local/indexing/main.nf +++ b/modules/local/indexing/main.nf @@ -33,4 +33,4 @@ process INDEXING { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/makesnv/main.nf b/modules/local/makesnv/main.nf index 8e2f89a..7fee485 100644 --- a/modules/local/makesnv/main.nf +++ b/modules/local/makesnv/main.nf @@ -28,4 +28,4 @@ process MAKE_SNV { perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/mpileup/main.nf b/modules/local/mpileup/main.nf index 09ef5de..d82c094 100644 --- a/modules/local/mpileup/main.nf +++ b/modules/local/mpileup/main.nf @@ -30,4 +30,4 @@ process MPILEUP { bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/smaltmap/main.nf b/modules/local/smaltmap/main.nf index abb3da2..7b07c88 100644 --- a/modules/local/smaltmap/main.nf +++ b/modules/local/smaltmap/main.nf @@ -35,4 +35,4 @@ process SMALT_MAP { smalt: \$( smalt version | grep "Version:" | sed 's/Version://' ) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/sortindexbams/main.nf b/modules/local/sortindexbams/main.nf index 9ac8740..4bfe13c 100644 --- a/modules/local/sortindexbams/main.nf +++ b/modules/local/sortindexbams/main.nf @@ -31,4 +31,4 @@ process SORT_INDEX_BAMS { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index cbf89dc..c42a327 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -15,10 +15,10 @@ process VCF2SNV_ALIGNMENT { input: val(consolidate_bcfs) - path(bcf) - path(new_invalid_positions) - path(refgenome) - path(consolidated_bcf_index) + path(bcf) + path(new_invalid_positions) + path(refgenome) + path(consolidated_bcf_index) output: path('snvAlignment.phy'), emit: snvAlignment @@ -28,7 +28,7 @@ process VCF2SNV_ALIGNMENT { script: """ - vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus 4 --output-base snvalign --fasta ${refgenome} ${consolidate_bcfs} + vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus 4 --output-base snvalign --fasta ${refgenome} ${consolidate_bcfs} mv snvalign-positions.tsv snvTable.tsv mv snvalign-stats.csv vcf2core.tsv if [[ -f snvalign.phy ]]; then @@ -45,4 +45,4 @@ process VCF2SNV_ALIGNMENT { perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ -} \ No newline at end of file +} diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index 6a51df2..d50072e 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -22,7 +22,7 @@ process VERIFYING_MAP_Q { script: """ - verify_mapping_quality.pl -c 4 --min-depth 5 --min-map 80 --output mappingQuality.txt ${bam_line} + verify_mapping_quality.pl -c 4 --min-depth 5 --min-map 80 --output mappingQuality.txt ${bam_line} cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -30,4 +30,4 @@ process VERIFYING_MAP_Q { perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ -} \ No newline at end of file +} From 700811f0b217d667e7a4a16f2445b85adbe93ff7 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 09:46:52 -0600 Subject: [PATCH 018/113] Linting --- .github/workflows/linting.yml | 2 +- .nf-core.yml | 1 + 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 9a48144..77831ca 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -22,7 +22,7 @@ jobs: run: npm install -g editorconfig-checker - name: Run ECLint check - run: editorconfig-checker -exclude README.md LICENSE-Apachev2.txt $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') + run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE') Prettier: runs-on: ubuntu-latest diff --git a/.nf-core.yml b/.nf-core.yml index 98f7248..42dfcaa 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -15,6 +15,7 @@ lint: - .github/ISSUE_TEMPLATE/bug_report.yml - .github/PULL_REQUEST_TEMPLATE.md - .github/workflows/branch.yml + - .github/workflows/linting.yml - assets/email_template.txt - docs/README.md - LICENSE From 2f0cdbeb3495d144029d058a7e15cd7b50106aa8 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 09:49:20 -0600 Subject: [PATCH 019/113] Trying to avoid EditorConfig check the LICENSE file. --- LICENSE-Apachev2.txt => LICENSE-Apachev2 | 0 1 file changed, 0 insertions(+), 0 deletions(-) rename LICENSE-Apachev2.txt => LICENSE-Apachev2 (100%) diff --git a/LICENSE-Apachev2.txt b/LICENSE-Apachev2 similarity index 100% rename from LICENSE-Apachev2.txt rename to LICENSE-Apachev2 From e4ac2adf298490cde57216b6deacc72689c545b7 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 09:54:53 -0600 Subject: [PATCH 020/113] Trying to avoid EditorConfig check the LICENSE file. --- .github/workflows/linting.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 77831ca..baa7f1b 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -22,7 +22,7 @@ jobs: run: npm install -g editorconfig-checker - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE') + run: editorconfig-checker -exclude LICENSE-Apachev2 README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE') Prettier: runs-on: ubuntu-latest From 9beb64865726928fe1cb66087b09374bff19c561 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 10:38:17 -0600 Subject: [PATCH 021/113] docker->singularity for testing --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index f9e3249..ebd49e7 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -56,4 +56,4 @@ jobs: - name: Nextflow run with test profile run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results From b0e96abdd636821955351b409e2d6fa3dd6e029f Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 11:00:54 -0600 Subject: [PATCH 022/113] phyml docker debugging --- .github/workflows/ci.yml | 2 +- modules/local/phyml/main.nf | 6 +++--- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index ebd49e7..f9e3249 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -56,4 +56,4 @@ jobs: - name: Nextflow run with test profile run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results + nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results diff --git a/modules/local/phyml/main.nf b/modules/local/phyml/main.nf index 831c53c..d370f08 100644 --- a/modules/local/phyml/main.nf +++ b/modules/local/phyml/main.nf @@ -9,8 +9,8 @@ Please refer to the README for more information. */ process PHYML { label 'process_low' - //container = "staphb/phyml:3.3.20220408" - container = "https://depot.galaxyproject.org/singularity/phyml:3.3.20211231--hee9e358_0" + container = "staphb/phyml:3.3.20220408" + //container = "https://depot.galaxyproject.org/singularity/phyml:3.3.20211231--hee9e358_0" input: path(snvAlignment_phy) @@ -22,7 +22,7 @@ process PHYML { script: """ - phyml -i ${snvAlignment_phy} --datatype nt --model GTR -v 0.0 --ts/tv e --nclasses 4 --alpha e --bootstrap -4 --quiet + phyml -i ${snvAlignment_phy} --datatype nt --model GTR -v 0.0 --ts/tv e --nclasses 4 --alpha e --bootstrap -4 mv snvAlignment.phy_phyml_stats.txt phylogeneticTreeStats.txt mv snvAlignment.phy_phyml_tree.txt phylogeneticTree.newick From 9ed0801a2c317995315f40ec3da1c14a0eae3657 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 11:17:10 -0600 Subject: [PATCH 023/113] Removing commented-out singularity container for PhyML. --- modules/local/phyml/main.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/phyml/main.nf b/modules/local/phyml/main.nf index d370f08..cccfead 100644 --- a/modules/local/phyml/main.nf +++ b/modules/local/phyml/main.nf @@ -10,7 +10,6 @@ Please refer to the README for more information. process PHYML { label 'process_low' container = "staphb/phyml:3.3.20220408" - //container = "https://depot.galaxyproject.org/singularity/phyml:3.3.20211231--hee9e358_0" input: path(snvAlignment_phy) From c128b5b0052024f793ebf5b23082895748f411b6 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 12:39:35 -0600 Subject: [PATCH 024/113] Changing PhyML to 3.3.20211231--hee9e358_1 docker container. --- modules/local/phyml/main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/phyml/main.nf b/modules/local/phyml/main.nf index cccfead..b6148b1 100644 --- a/modules/local/phyml/main.nf +++ b/modules/local/phyml/main.nf @@ -9,7 +9,7 @@ Please refer to the README for more information. */ process PHYML { label 'process_low' - container = "staphb/phyml:3.3.20220408" + container = "quay.io/biocontainers/phyml:3.3.20211231--hee9e358_0" input: path(snvAlignment_phy) @@ -21,7 +21,7 @@ process PHYML { script: """ - phyml -i ${snvAlignment_phy} --datatype nt --model GTR -v 0.0 --ts/tv e --nclasses 4 --alpha e --bootstrap -4 + phyml -i ${snvAlignment_phy} --datatype nt --model GTR -v 0.0 --ts/tv e --nclasses 4 --alpha e --bootstrap -4 --quiet mv snvAlignment.phy_phyml_stats.txt phylogeneticTreeStats.txt mv snvAlignment.phy_phyml_tree.txt phylogeneticTree.newick From 583f76f80a520001c0186e1e8df45c12933e39de Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 12:46:07 -0600 Subject: [PATCH 025/113] Trigger CI --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index ca2f74c..fa0dd45 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ # SNVPhyl nf-core Pipeline -This is the in-development nf-core-based pipeline for SNVPhyl. +This is the in-development nf-core-based pipeline for SNVPhyl. # Legal From 94e6104ae6edc84bfe980894c58fb0ebc4b57492 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 10 Jan 2024 12:48:09 -0600 Subject: [PATCH 026/113] Linting --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index fa0dd45..ca2f74c 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ # SNVPhyl nf-core Pipeline -This is the in-development nf-core-based pipeline for SNVPhyl. +This is the in-development nf-core-based pipeline for SNVPhyl. # Legal From dc409366c5b87fda137e914d0a38dbe570aa9533 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 11 Jan 2024 12:00:14 -0600 Subject: [PATCH 027/113] Adding variable CPUs. --- modules/local/bcftoolscall/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/bcftoolscall/main.nf b/modules/local/bcftoolscall/main.nf index 2bddeb8..dec7e05 100644 --- a/modules/local/bcftoolscall/main.nf +++ b/modules/local/bcftoolscall/main.nf @@ -23,7 +23,7 @@ process BCFTOOLS_CALL { def prefix = task.ext.prefix ?: "${meta.id}" """ bcftools index -f ${mpileup_vcf_gz} - bcftools call --ploidy 1 --threads 4 --output ${prefix}_mpileup.bcf --output-type b --consensus-caller ${mpileup_vcf_gz} + bcftools call --ploidy 1 --threads ${$task.cpus} --output ${prefix}_mpileup.bcf --output-type b --consensus-caller ${mpileup_vcf_gz} cat <<-END_VERSIONS > versions.yml "${task.process}": From f9490ade3d3822680e9520cb26bac148c1cae8a4 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 11 Jan 2024 12:05:23 -0600 Subject: [PATCH 028/113] Fixing variable CPUs. --- modules/local/bcftoolscall/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/bcftoolscall/main.nf b/modules/local/bcftoolscall/main.nf index dec7e05..0722f7b 100644 --- a/modules/local/bcftoolscall/main.nf +++ b/modules/local/bcftoolscall/main.nf @@ -23,7 +23,7 @@ process BCFTOOLS_CALL { def prefix = task.ext.prefix ?: "${meta.id}" """ bcftools index -f ${mpileup_vcf_gz} - bcftools call --ploidy 1 --threads ${$task.cpus} --output ${prefix}_mpileup.bcf --output-type b --consensus-caller ${mpileup_vcf_gz} + bcftools call --ploidy 1 --threads ${task.cpus} --output ${prefix}_mpileup.bcf --output-type b --consensus-caller ${mpileup_vcf_gz} cat <<-END_VERSIONS > versions.yml "${task.process}": From ffc0dac33d32aa976fd618c93c507441aeae998c Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 11 Jan 2024 12:12:21 -0600 Subject: [PATCH 029/113] Adding coverage, quality mapping, and VCF abundance pipeline parameters. --- modules/local/consolidatebcfs/main.nf | 2 +- nextflow.config | 6 ++++++ nextflow_schema.json | 18 ++++++++++++++++++ 3 files changed, 25 insertions(+), 1 deletion(-) diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index bae7fd3..9ff6a9c 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -25,7 +25,7 @@ process CONSOLIDATE_BCFS { script: def prefix = task.ext.prefix ?: "${meta.id}" """ - consolidate_vcfs.pl --coverage-cutoff 5 --min-mean-mapping 30 --snv-abundance-ratio 0.75 --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf + consolidate_vcfs.pl --coverage-cutoff ${params.coverage_cutoff} --min-mean-mapping ${params.min_mean_mapping} --snv-abundance-ratio ${params.snv_abundance_ratio} --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf bcftools index -f ${prefix}_consolidated.bcf cat <<-END_VERSIONS > versions.yml diff --git a/nextflow.config b/nextflow.config index 206bb08..1862ff4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,8 +12,14 @@ params { // Input options input = null refgenome = "${baseDir}/assets/reference.fasta" + + // Pipeline parameters window_size = 11 density_threshold = 2 + coverage_cutoff = 5 + min_mean_mapping = 30 + snv_abundance_ratio = 0.75 + // Boilerplate options outdir = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 811d1be..aed1e90 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -60,6 +60,24 @@ "default": 2, "description": "Used during BCF consolidation.", "fa_icon": "fas fa-desktop" + }, + "coverage_cutoff": { + "type": "integer", + "default": 5, + "fa_icon": "fas fa-desktop", + "description": "The minimum depth of coverage for a VCF to be accepted." + }, + "min_mean_mapping": { + "type": "integer", + "default": 30, + "fa_icon": "fas fa-desktop", + "description": "The minimum mean mapping quality." + }, + "snv_abundance_ratio": { + "type": "number", + "default": 0.75, + "fa_icon": "fas fa-desktop", + "description": "The SNV abundance ratio." } }, "fa_icon": "fas fa-terminal" From 386cd01570aa4bbe18ee129f3be75d593da51889 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 11 Jan 2024 12:20:10 -0600 Subject: [PATCH 030/113] Adding pipeline parameters for the find repeats module. --- modules/local/findrepeats/main.nf | 2 +- nextflow.config | 3 ++- nextflow_schema.json | 12 ++++++++++++ 3 files changed, 15 insertions(+), 2 deletions(-) diff --git a/modules/local/findrepeats/main.nf b/modules/local/findrepeats/main.nf index 7e767ad..7701530 100644 --- a/modules/local/findrepeats/main.nf +++ b/modules/local/findrepeats/main.nf @@ -21,7 +21,7 @@ process FIND_REPEATS { script: """ - find-repeats.pl ${refgenome} --min-length 150 --min-pid 90 > invalid_positions.bed + find-repeats.pl ${refgenome} --min-length ${params.min_repeat_length} --min-pid ${params.min_repeat_pid} > invalid_positions.bed cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index 1862ff4..8f6197d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -19,7 +19,8 @@ params { coverage_cutoff = 5 min_mean_mapping = 30 snv_abundance_ratio = 0.75 - + min_repeat_length = 150 + min_repeat_pid = 90 // Boilerplate options outdir = null diff --git a/nextflow_schema.json b/nextflow_schema.json index aed1e90..a9d78e3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -78,6 +78,18 @@ "default": 0.75, "fa_icon": "fas fa-desktop", "description": "The SNV abundance ratio." + }, + "min_repeat_length": { + "type": "integer", + "default": 150, + "fa_icon": "fas fa-desktop", + "description": "The minimum length when identifying repeats." + }, + "min_repeat_pid": { + "type": "integer", + "default": 90, + "fa_icon": "fas fa-desktop", + "description": "The minimum percent identity when identifying repeats." } }, "fa_icon": "fas fa-terminal" From 0d9c39ab3aea4a638bf1657de0a24ecfbbaf742b Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 11 Jan 2024 12:23:10 -0600 Subject: [PATCH 031/113] Adding task.cpus to mpileup. --- modules/local/mpileup/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/mpileup/main.nf b/modules/local/mpileup/main.nf index d82c094..2beb7d8 100644 --- a/modules/local/mpileup/main.nf +++ b/modules/local/mpileup/main.nf @@ -23,7 +23,7 @@ process MPILEUP { script: def prefix = task.ext.prefix ?: "${meta.id}" """ - bcftools mpileup --threads 4 --fasta-ref ${refgenome} -A -B -C 0 -d 1024 -q 0 -Q 0 --output-type v -I --output ${prefix}_mpileup.vcf ${sorted_bams} + bcftools mpileup --threads ${task.cpus} --fasta-ref ${refgenome} -A -B -C 0 -d 1024 -q 0 -Q 0 --output-type v -I --output ${prefix}_mpileup.vcf ${sorted_bams} cat <<-END_VERSIONS > versions.yml "${task.process}": From 785cf71513701783c910bcb910533693ac3f2c34 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 11 Jan 2024 12:34:53 -0600 Subject: [PATCH 032/113] Adding parameters for verifymapq(uality). Harmonized with consolidatebcfs. --- modules/local/consolidatebcfs/main.nf | 2 +- modules/local/verifyingmapq/main.nf | 2 +- nextflow.config | 5 +++-- nextflow_schema.json | 12 ++++++++++-- 4 files changed, 15 insertions(+), 6 deletions(-) diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index 9ff6a9c..1b8663a 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -25,7 +25,7 @@ process CONSOLIDATE_BCFS { script: def prefix = task.ext.prefix ?: "${meta.id}" """ - consolidate_vcfs.pl --coverage-cutoff ${params.coverage_cutoff} --min-mean-mapping ${params.min_mean_mapping} --snv-abundance-ratio ${params.snv_abundance_ratio} --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf + consolidate_vcfs.pl --coverage-cutoff ${params.min_coverage_depth} --min-mean-mapping ${params.min_mean_mapping_quality} --snv-abundance-ratio ${params.snv_abundance_ratio} --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf bcftools index -f ${prefix}_consolidated.bcf cat <<-END_VERSIONS > versions.yml diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index d50072e..9ae805c 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -22,7 +22,7 @@ process VERIFYING_MAP_Q { script: """ - verify_mapping_quality.pl -c 4 --min-depth 5 --min-map 80 --output mappingQuality.txt ${bam_line} + verify_mapping_quality.pl -c 4 --min-depth ${params.min_coverage_depth} --min-map ${params.min_mapping_percent_cov} --output mappingQuality.txt ${bam_line} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index 8f6197d..10eab56 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,8 +16,9 @@ params { // Pipeline parameters window_size = 11 density_threshold = 2 - coverage_cutoff = 5 - min_mean_mapping = 30 + min_coverage_depth = 5 + min_mapping_percent_cov = 80 + min_mean_mapping_quality = 30 snv_abundance_ratio = 0.75 min_repeat_length = 150 min_repeat_pid = 90 diff --git a/nextflow_schema.json b/nextflow_schema.json index a9d78e3..d260677 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -61,18 +61,26 @@ "description": "Used during BCF consolidation.", "fa_icon": "fas fa-desktop" }, - "coverage_cutoff": { + "min_coverage_depth": { "type": "integer", "default": 5, "fa_icon": "fas fa-desktop", "description": "The minimum depth of coverage for a VCF to be accepted." }, - "min_mean_mapping": { + "min_mean_mapping_quality": { "type": "integer", "default": 30, "fa_icon": "fas fa-desktop", "description": "The minimum mean mapping quality." }, + "min_mapping_percent_cov": { + "type": "integer", + "default": 80, + "description": "The minimum mapping percent coverage.", + "minimum": 0, + "maximum": 100, + "fa_icon": "fas fa-desktop" + }, "snv_abundance_ratio": { "type": "number", "default": 0.75, From e8252e6847eeb0aad4caa3cbf1ba760bf2c465ed Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 11 Jan 2024 12:37:07 -0600 Subject: [PATCH 033/113] Added snvphyl-tools version. --- modules/local/consolidatebcfs/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index 1b8663a..737e80a 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -30,7 +30,7 @@ process CONSOLIDATE_BCFS { cat <<-END_VERSIONS > versions.yml "${task.process}": - snvphyl-tools: + snvphyl-tools: 1.8.2 bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) END_VERSIONS """ From 5cd9642856e7f940ee90ea252e1d6f228deff9d6 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 09:02:37 -0600 Subject: [PATCH 034/113] Adding variable CPUs. --- modules/local/verifyingmapq/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index 9ae805c..e63c13a 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -22,7 +22,7 @@ process VERIFYING_MAP_Q { script: """ - verify_mapping_quality.pl -c 4 --min-depth ${params.min_coverage_depth} --min-map ${params.min_mapping_percent_cov} --output mappingQuality.txt ${bam_line} + verify_mapping_quality.pl -c ${task.cpus} --min-depth ${params.min_coverage_depth} --min-map ${params.min_mapping_percent_cov} --output mappingQuality.txt ${bam_line} cat <<-END_VERSIONS > versions.yml "${task.process}": From 220e2acfa84260dd11bf767cbdab52a5c764d015 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 09:06:26 -0600 Subject: [PATCH 035/113] Adding version number to multiple snvphyl-tools version outputs. --- modules/local/filterfreebayes/main.nf | 2 +- modules/local/filterstats/main.nf | 2 +- modules/local/findrepeats/main.nf | 2 +- modules/local/makesnv/main.nf | 2 +- modules/local/vcf2snvalignment/main.nf | 2 +- modules/local/verifyingmapq/main.nf | 2 +- 6 files changed, 6 insertions(+), 6 deletions(-) diff --git a/modules/local/filterfreebayes/main.nf b/modules/local/filterfreebayes/main.nf index 3ad851f..b458f3a 100644 --- a/modules/local/filterfreebayes/main.nf +++ b/modules/local/filterfreebayes/main.nf @@ -26,7 +26,7 @@ process FILTER_FREEBAYES { cat <<-END_VERSIONS > versions.yml "${task.process}": - snvphyl-tools: + snvphyl-tools: 1.8.2 perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ diff --git a/modules/local/filterstats/main.nf b/modules/local/filterstats/main.nf index 1317c43..4c152e8 100644 --- a/modules/local/filterstats/main.nf +++ b/modules/local/filterstats/main.nf @@ -24,7 +24,7 @@ process FILTER_STATS { cat <<-END_VERSIONS > versions.yml "${task.process}": - snvphyl-tools: + snvphyl-tools: 1.8.2 perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ diff --git a/modules/local/findrepeats/main.nf b/modules/local/findrepeats/main.nf index 7701530..aa48985 100644 --- a/modules/local/findrepeats/main.nf +++ b/modules/local/findrepeats/main.nf @@ -25,7 +25,7 @@ process FIND_REPEATS { cat <<-END_VERSIONS > versions.yml "${task.process}": - snvphyl-tools: + snvphyl-tools: 1.8.2 perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) """ } diff --git a/modules/local/makesnv/main.nf b/modules/local/makesnv/main.nf index 7fee485..a4c9a2a 100644 --- a/modules/local/makesnv/main.nf +++ b/modules/local/makesnv/main.nf @@ -24,7 +24,7 @@ process MAKE_SNV { cat <<-END_VERSIONS > versions.yml "${task.process}": - snvphyl-tools: + snvphyl-tools: 1.8.2 perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index c42a327..50d5902 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -41,7 +41,7 @@ process VCF2SNV_ALIGNMENT { cat <<-END_VERSIONS > versions.yml "${task.process}": - snvphyl-tools: + snvphyl-tools: 1.8.2 perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index e63c13a..8d10dab 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -26,7 +26,7 @@ process VERIFYING_MAP_Q { cat <<-END_VERSIONS > versions.yml "${task.process}": - snvphyl-tools: + snvphyl-tools: 1.8.2 perl: \$(perl --version | grep "This is perl" | sed 's/.*(\\(.*\\))/\\1/' | cut -d " " -f1) END_VERSIONS """ From 10f5a92e2acca18d41641bc59bc42bc711120612 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 11:54:20 -0600 Subject: [PATCH 036/113] Removing unneeded tests. --- .../local/generatesamplejson/main.nf.test | 40 --------------- .../local/generatesummary/main.nf.test | 37 -------------- .../local/iridanextoutput/main.nf.test | 51 ------------------- .../local/simplifyiridajson/main.nf.test | 41 --------------- 4 files changed, 169 deletions(-) delete mode 100644 tests/modules/local/generatesamplejson/main.nf.test delete mode 100644 tests/modules/local/generatesummary/main.nf.test delete mode 100644 tests/modules/local/iridanextoutput/main.nf.test delete mode 100644 tests/modules/local/simplifyiridajson/main.nf.test diff --git a/tests/modules/local/generatesamplejson/main.nf.test b/tests/modules/local/generatesamplejson/main.nf.test deleted file mode 100644 index ba25484..0000000 --- a/tests/modules/local/generatesamplejson/main.nf.test +++ /dev/null @@ -1,40 +0,0 @@ -nextflow_process { - - name "Test Process GENERATE_SAMPLE_JSON" - script "modules/local/generatesamplejson/main.nf" - process "GENERATE_SAMPLE_JSON" - - test("Basic execution, check output.") { - - when { - params { - outdir = "tests/results" - } - process { - """ - input[0] = new Tuple(["id": "SAMPLE1"], [file("sample1_R1.fastq.gz"), file("sample1_R2.fastq.gz")], file("SAMPLE1.assembly.fa.gz")) - """ - } - } - - then { - assert process.success - - with(process.out) { - // check if emitted output has been created - assert json.size() == 1 - - // parse output json file - def sample_json_string = path(json.get(0)[1]).linesGzip.join("\n") - def parser = new groovy.json.JsonSlurper() - def sample_json = parser.parseText(sample_json_string) - - assert sample_json.files.samples.SAMPLE1[0].path.equals("assembly/SAMPLE1.assembly.fa.gz") - assert sample_json.metadata.samples.SAMPLE1.reads[0].equals("sample1_R1.fastq.gz") - assert sample_json.metadata.samples.SAMPLE1.reads[1].equals("sample1_R2.fastq.gz") - } - } - - } - -} diff --git a/tests/modules/local/generatesummary/main.nf.test b/tests/modules/local/generatesummary/main.nf.test deleted file mode 100644 index 22b4c4a..0000000 --- a/tests/modules/local/generatesummary/main.nf.test +++ /dev/null @@ -1,37 +0,0 @@ -nextflow_process { - - name "Test Process GENERATE_SUMMARY" - script "modules/local/generatesummary/main.nf" - process "GENERATE_SUMMARY" - - test("Basic execution, check output.") { - - when { - params { - outdir = "tests/results" - } - process { - """ - input[0] = [new Tuple(["id": "SAMPLE1"], [file("sample1_R1.fastq.gz"), file("sample1_R2.fastq.gz")], file("SAMPLE1.assembly.fa.gz"))] - """ - } - } - - then { - assert process.success - - with(process.out) { - // check if emitted output has been created - assert summary.size() == 1 - - assert path(summary.get(0)).linesGzip[0].equals("SNVPhyl Pipeline Summary") - assert path(summary.get(0)).linesGzip[4].equals("SAMPLE1:") - assert path(summary.get(0)).linesGzip[5].contains("reads.1: ") - assert path(summary.get(0)).linesGzip[6].contains("reads.2: ") - assert path(summary.get(0)).linesGzip[7].contains("assembly: ") - } - } - - } - -} diff --git a/tests/modules/local/iridanextoutput/main.nf.test b/tests/modules/local/iridanextoutput/main.nf.test deleted file mode 100644 index 72808ab..0000000 --- a/tests/modules/local/iridanextoutput/main.nf.test +++ /dev/null @@ -1,51 +0,0 @@ -nextflow_process { - - name "Test Process IRIDA_NEXT_OUTPUT" - script "modules/local/iridanextoutput/main.nf" - process "IRIDA_NEXT_OUTPUT" - - test("Basic execution, check output.") { - - when { - params { - outdir = "tests/results" - } - process { - """ - input[0] = [file("$baseDir/tests/data/SAMPLE1.simple.json.gz"), file("$baseDir/tests/data/SAMPLE2.simple.json.gz"), file("$baseDir/tests/data/SAMPLE3.simple.json.gz")] - """ - } - } - - then { - assert process.success - - with(process.out) { - // check if emitted output has been created - assert output_json.size() == 1 - - // parse output json file - def json_string = path(output_json.get(0)).linesGzip.join("\n") - def parser = new groovy.json.JsonSlurper() - def irida_json = parser.parseText(json_string) - - assert irida_json.files.global[0].path.equals("summary/summary.txt.gz") - - assert irida_json.files.samples.SAMPLE1[0].path.equals("assembly/SAMPLE1.assembly.fa.gz") - assert irida_json.files.samples.SAMPLE2[0].path.equals("assembly/SAMPLE2.assembly.fa.gz") - assert irida_json.files.samples.SAMPLE3[0].path.equals("assembly/SAMPLE3.assembly.fa.gz") - - assert irida_json.metadata.samples.SAMPLE1.'reads.1'.equals("sample1_R1.fastq.gz") - assert irida_json.metadata.samples.SAMPLE1.'reads.2'.equals("sample1_R2.fastq.gz") - - assert irida_json.metadata.samples.SAMPLE2.'reads.1'.equals("sample2_R1.fastq.gz") - assert irida_json.metadata.samples.SAMPLE2.'reads.2'.equals("sample2_R2.fastq.gz") - - assert irida_json.metadata.samples.SAMPLE3.'reads.1'.equals("sample1_R1.fastq.gz") - assert irida_json.metadata.samples.SAMPLE3.'reads.2'.equals("null") - } - } - - } - -} diff --git a/tests/modules/local/simplifyiridajson/main.nf.test b/tests/modules/local/simplifyiridajson/main.nf.test deleted file mode 100644 index 7d61567..0000000 --- a/tests/modules/local/simplifyiridajson/main.nf.test +++ /dev/null @@ -1,41 +0,0 @@ -nextflow_process { - - name "Test Process SIMPLIFY_IRIDA_JSON" - script "modules/local/simplifyiridajson/main.nf" - process "SIMPLIFY_IRIDA_JSON" - - test("Basic execution, check output.") { - - when { - params { - outdir = "tests/results" - } - process { - """ - input[0] = new Tuple(["id": "SAMPLE1"], file("$baseDir/tests/data/SAMPLE1.json.gz")) - """ - } - } - - then { - assert process.success - - with(process.out) { - // check if emitted output has been created - assert simple_json.size() == 1 - - // parse output json file - def json_string = path(simple_json.get(0)[1]).linesGzip.join("\n") - def parser = new groovy.json.JsonSlurper() - def json_simple = parser.parseText(json_string) - - assert json_simple.files.samples.SAMPLE1[0].path.equals("assembly/SAMPLE1.assembly.fa.gz") - - assert json_simple.metadata.samples.SAMPLE1.'reads.1'.equals("sample1_R1.fastq.gz") - assert json_simple.metadata.samples.SAMPLE1.'reads.2'.equals("sample1_R2.fastq.gz") - } - } - - } - -} From daef5c42c873b6a5671c80af25dac9e924811c48 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 11:55:25 -0600 Subject: [PATCH 037/113] Adding basic integration test. --- nf-test.config | 6 +++- tests/workflows/snvphylnfc.nf.test | 58 ++++++++++++++++++++++++++++++ 2 files changed, 63 insertions(+), 1 deletion(-) create mode 100644 tests/workflows/snvphylnfc.nf.test diff --git a/nf-test.config b/nf-test.config index 870799d..f237cf3 100644 --- a/nf-test.config +++ b/nf-test.config @@ -3,6 +3,10 @@ config { testsDir "tests" workDir ".nf-test" configFile "tests/nextflow.config" - profile "" + profile "docker" + + stage { + symlink "nextflow_schema.json" + } } diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test new file mode 100644 index 0000000..3531c4e --- /dev/null +++ b/tests/workflows/snvphylnfc.nf.test @@ -0,0 +1,58 @@ +nextflow_workflow { + + name "Full Integration Tests for PHACNML_SNVPHYL" + script "workflows/snvphylnfc.nf" + workflow "SNVPHYL" + + test("basic integration test") { + + when { + params { + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/samplesheet.csv" + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + outdir = "results" + } + workflow {} + } + + then { + assert workflow.success + assert path("$launchDir/results").exists() + + def lines = [] + + // check FILTER_STATS output + lines = path("$launchDir/results/filter/filterStats.txt").readLines() + + assert lines.contains("Number of sites used to generate phylogeny: 2") + assert lines.contains("Total number of sites identified: 2") + assert lines.contains("Number of sites filtered: 0") + + // check PHYML output + lines = path("$launchDir/results/phyml/phylogeneticTreeStats.txt").readLines() + + assert lines.contains(". Model of nucleotides substitution: \tGTR") + assert lines.contains(" - f(A)= 0.75000") + assert lines.contains(" - f(C)= 0.12500") + assert lines.contains(" - f(G)= 0.01000") + assert lines.contains(" - f(T)= 0.12500") + + lines = path("$launchDir/results/phyml/phylogeneticTree.newick").readLines() + + assert lines.join("\n").contains("SAMPLE1") + assert lines.join("\n").contains("SAMPLE2") + assert lines.join("\n").contains("SAMPLE3") + + // check MAKE_SNV output + lines = path("$launchDir/results/make/snvMatrix.tsv").readLines() + + assert lines[0] == "strain\tSAMPLE2\tSAMPLE3\tSAMPLE1\treference\t" + assert lines.contains("SAMPLE1\t1\t1\t0\t0\t") + assert lines.contains("SAMPLE2\t0\t2\t1\t1\t") + assert lines.contains("SAMPLE3\t2\t0\t1\t1\t") + + } + + } + +} From b99746a70b9bed81b47500a216d2414b13ac6af5 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 12:12:24 -0600 Subject: [PATCH 038/113] Added parameter bounds. --- nextflow_schema.json | 23 ++++++++++++++++------- 1 file changed, 16 insertions(+), 7 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index d260677..bc93334 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -53,25 +53,29 @@ "type": "integer", "default": 11, "description": "Used during BCF consolidation.", - "fa_icon": "fas fa-desktop" + "fa_icon": "fas fa-desktop", + "minimum": 1 }, "density_threshold": { "type": "integer", "default": 2, "description": "Used during BCF consolidation.", - "fa_icon": "fas fa-desktop" + "fa_icon": "fas fa-desktop", + "minimum": 1 }, "min_coverage_depth": { "type": "integer", "default": 5, "fa_icon": "fas fa-desktop", - "description": "The minimum depth of coverage for a VCF to be accepted." + "description": "The minimum depth of coverage for a VCF to be accepted.", + "minimum": 1 }, "min_mean_mapping_quality": { "type": "integer", "default": 30, "fa_icon": "fas fa-desktop", - "description": "The minimum mean mapping quality." + "description": "The minimum mean mapping quality.", + "minimum": 1 }, "min_mapping_percent_cov": { "type": "integer", @@ -85,19 +89,24 @@ "type": "number", "default": 0.75, "fa_icon": "fas fa-desktop", - "description": "The SNV abundance ratio." + "description": "The SNV abundance ratio.", + "minimum": 0, + "maximum": 1 }, "min_repeat_length": { "type": "integer", "default": 150, "fa_icon": "fas fa-desktop", - "description": "The minimum length when identifying repeats." + "description": "The minimum length when identifying repeats.", + "minimum": 1 }, "min_repeat_pid": { "type": "integer", "default": 90, "fa_icon": "fas fa-desktop", - "description": "The minimum percent identity when identifying repeats." + "description": "The minimum percent identity when identifying repeats.", + "minimum": 0, + "maximum": 100 } }, "fa_icon": "fas fa-terminal" From e75f97b18ff93a4adf4da0f7007190b722e24f7f Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 12:36:44 -0600 Subject: [PATCH 039/113] Format file-path and FASTA extension pattern. --- nextflow_schema.json | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index bc93334..6bed7bc 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -32,7 +32,9 @@ "refgenome": { "type": "string", "description": "The file path to the FASTA-format reference genome to use.", - "fa_icon": "fas fa-file" + "fa_icon": "fas fa-file", + "pattern": "^\\S+\\.(fa|fas|fasta)$", + "format": "file-path" }, "email": { "type": "string", From 50a53ba60156e475ae2697cd38db9bdb13eed572 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 16:02:11 -0600 Subject: [PATCH 040/113] Removing configs for modules that are not used. --- conf/modules.config | 24 ------------------------ 1 file changed, 24 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index cbe33ab..c4cb547 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,30 +22,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: GENERATE_SUMMARY { - publishDir = [ - path: { ["${params.outdir}", "${task.summary_directory_name}"].join(File.separator) }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: IRIDA_NEXT_OUTPUT { - publishDir = [ - path: { "${params.outdir}" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, From b416590f23715857383835f66101cc42d37d898b Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 16:02:30 -0600 Subject: [PATCH 041/113] Removing unused modules. --- workflows/snvphylnfc.nf | 4 ---- 1 file changed, 4 deletions(-) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 8401668..8b76624 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -43,10 +43,6 @@ WorkflowSnvphylnfc.initialise(params, log) // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // include { INPUT_CHECK } from '../subworkflows/local/input_check' -include { GENERATE_SAMPLE_JSON } from '../modules/local/generatesamplejson/main' -include { SIMPLIFY_IRIDA_JSON } from '../modules/local/simplifyiridajson/main' -include { IRIDA_NEXT_OUTPUT } from '../modules/local/iridanextoutput/main' -include { GENERATE_SUMMARY } from '../modules/local/generatesummary/main' include { INDEXING } from '../modules/local/indexing/main' include { FIND_REPEATS } from '../modules/local/findrepeats/main' include { SMALT_MAP } from '../modules/local/smaltmap/main' From 73b74badd018bea7c10828e96ccba774004f5ef9 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 15 Jan 2024 16:04:28 -0600 Subject: [PATCH 042/113] Adding module test for freebayes --- tests/data/SAMPLE1_sorted.bam | Bin 0 -> 408 bytes tests/data/SAMPLE2_sorted.bam | Bin 0 -> 409 bytes tests/data/SAMPLE3_sorted.bam | Bin 0 -> 419 bytes tests/modules/local/freebayes/main.nf.test | 113 +++++++++++++++++++++ 4 files changed, 113 insertions(+) create mode 100644 tests/data/SAMPLE1_sorted.bam create mode 100644 tests/data/SAMPLE2_sorted.bam create mode 100644 tests/data/SAMPLE3_sorted.bam create mode 100644 tests/modules/local/freebayes/main.nf.test diff --git a/tests/data/SAMPLE1_sorted.bam b/tests/data/SAMPLE1_sorted.bam new file mode 100644 index 0000000000000000000000000000000000000000..fd1077695eff703c65c8c0a028cb32a9a847b754 GIT binary patch literal 408 zcmb2|=3rp}f&Xj_PR>jW#~5t;&T<_x5NKJv{fp)^Rw1F4T$Wp-0vE2G+7RAp+Uixm z<=dm~%1;k_LaJ^&O`b9LX6Ou`do2?Z#4iS~Qu)5pZpEJ5-Pt#1+^cG|3!1y?oY=O_ zzImB*RxK5Xn~?j`F6jKN5Zkkt?00Y+oL=L2{o{fKX5|~0#DkknKOEG$qZ`4g*}||p z@mU!==eMQ@#T&$|d^S}m|9qeJrq$9rZbth50u$*Ivv-`gHDBCav#h0t@uAH3?khmo z%cFT?3qy8_1B05hzoJ`P3R|*@p}_442ODNKF7ylxbn#rYbm`K-MS&iH&KnLNc6Pe3 z*g0T@V{^%a=d(GD?iyMMr#LDL_8Ca=^clzju_O@70jW#~5t;&hj-G2()x>|0MF6DVNL9v+B_P6`TsoI>UoEs7U=3 z-0>r6UX4YzflS%wR3q!nt5YBC;Z(d>=X`$UmkNigfnvUwWAnGl^+>N24qf@iC(|le zyzSUZp4NiJIqv+@H^YA!UeNj5+$&)7gnL(?JL}ZEgI-T|F|8JCu)QF)LE1)<<%aCO zyRtSL#98Jyn+BX#>E3Sl|E|y86Z&%crS>+uKb3d({k^l*c;|_9w-e$s`e!Y@0(8AR znm4vGWT!YVs7d=Py0xXSC94<;+@5f-VP@k(Pv<}v&qYg@E)851=n?3=;qYN+rwfan z16DXTmpphr`y_|SO+$;z3?8OJ4>=Yee8BM-h$jQ_Wgy-R#Giq<8;HGsStl8FDWCiI z|9?FLm$r{Pk3(YX%MM13H3qVV46UNel-U++I8<_kK_qNOpG3nUuFHbV0TGE-P0w0I V8N6h;gQGYxT`kSP42}yB0RX8cpA!H8 literal 0 HcmV?d00001 diff --git a/tests/data/SAMPLE3_sorted.bam b/tests/data/SAMPLE3_sorted.bam new file mode 100644 index 0000000000000000000000000000000000000000..dff4294faf43d26570b3e73f6fc6e357f3f04822 GIT binary patch literal 419 zcmb2|=3rp}f&Xj_PR>jW#~5t;&hj-G2()x>|0MF6DVJ-Jifv=k6&3;KM^{61INRi1 z_4dtBmp>=P(|)&R=9zPEw0zc>E2`XZ{W8U?z1qb7#G==~vahakyzN-Gq*ALo_Ep^W z)@6lSwTf>PZeOZZncusLeb&-?1Hq=>HJ;Z$u2>LQzClR*YQxqK2eR&%MzFebFqSoE zm+`Z|VLd3GAp6PSsvZXQ%SbX+Q6n8Sgxi?sh`lpK;&*D?r!F zqj_ULLw1S-gPOFzqFY-ETe6Cw!0ibK8)h~x^b8Di@m#cY>C(VOfgXX*8x9|KcDk_G z*<*!cbIF6}vrlrE+%&Yf%-~@v^pIom!3P|Vfp{_yUk2jMK>QhqyMfrdrcXfSx*(5$ zyF_y7krWa4Ie-8E|IZ*2Hlt6X;SkqlLFRynM5`nQE^Qxo9*4x%mmQ26YYb!!8Cpe` gDYGruaHxdkEVnkp?o);fqBt?#FU`OVjuj9A09JXW9smFU literal 0 HcmV?d00001 diff --git a/tests/modules/local/freebayes/main.nf.test b/tests/modules/local/freebayes/main.nf.test new file mode 100644 index 0000000..17cec4a --- /dev/null +++ b/tests/modules/local/freebayes/main.nf.test @@ -0,0 +1,113 @@ +nextflow_process { + + name "Test Process FREEBAYES" + script "modules/local/freebayes/main.nf" + process "FREEBAYES" + + test("SAMPLE1") { + + when { + params { + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + outdir = "results" + } + process { + """ + input[0] = new Tuple(["id": "SAMPLE1"], file("$baseDir/tests/data/SAMPLE1_sorted.bam")) + input[1] = params.refgenome + """ + } + } + + then { + assert process.success + + with(process.out) { + + meta_and_path = vcf_files[0] + meta_component = meta_and_path[0] + path_component = meta_and_path[1] + + assert path(path_component).exists() + + def lines = path(path_component).readLines() + assert lines.contains("##reference=reference.fasta") + assert lines.contains("##contig=") + assert lines[-1] == "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tunknown" + } + } + + } + + test("SAMPLE2") { + + when { + params { + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + outdir = "results" + } + process { + """ + input[0] = new Tuple(["id": "SAMPLE2"], file("$baseDir/tests/data/SAMPLE2_sorted.bam")) + input[1] = params.refgenome + """ + } + } + + then { + assert process.success + + with(process.out) { + + meta_and_path = vcf_files[0] + meta_component = meta_and_path[0] + path_component = meta_and_path[1] + + assert path(path_component).exists() + + def lines = path(path_component).readLines() + assert lines.contains("##reference=reference.fasta") + assert lines.contains("##contig=") + assert lines[-2] == "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tunknown" + assert lines[-1] == "reference\t4\t.\tA\tT\t582.476\t.\tAB=0;ABP=0;AC=1;AF=1;AN=1;AO=18;CIGAR=1X;DP=18;DPB=18;DPRA=0;EPP=3.0103;EPPR=0;GTI=0;LEN=1;MEANALT=1;MQM=60;MQMR=0;NS=1;NUMALT=1;ODDS=134.12;PAIRED=1;PAIREDR=0;PAO=0;PQA=0;PQR=0;PRO=0;QA=720;QR=0;RO=0;RPL=0;RPP=42.0968;RPPR=0;RPR=18;RUN=1;SAF=9;SAP=3.0103;SAR=9;SRF=0;SRP=0;SRR=0;TYPE=snp\tGT:DP:AD:RO:QR:AO:QA:GL\t1:18:0,18:0:0:18:720:-65.1296,0" + } + } + + } + + test("SAMPLE3") { + + when { + params { + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + outdir = "results" + } + process { + """ + input[0] = new Tuple(["id": "SAMPLE3"], file("$baseDir/tests/data/SAMPLE3_sorted.bam")) + input[1] = params.refgenome + """ + } + } + + then { + assert process.success + + with(process.out) { + + meta_and_path = vcf_files[0] + meta_component = meta_and_path[0] + path_component = meta_and_path[1] + + assert path(path_component).exists() + + def lines = path(path_component).readLines() + assert lines.contains("##reference=reference.fasta") + assert lines.contains("##contig=") + assert lines[-2] == "#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tunknown" + assert lines[-1] == "reference\t48\t.\tA\tC\t453.194\t.\tAB=0;ABP=0;AC=1;AF=1;AN=1;AO=16;CIGAR=1X;DP=18;DPB=18;DPRA=0;EPP=3.0103;EPPR=3.0103;GTI=0;LEN=1;MEANALT=1;MQM=60;MQMR=60;NS=1;NUMALT=1;ODDS=104.352;PAIRED=1;PAIREDR=1;PAO=0;PQA=0;PQR=0;PRO=0;QA=640;QR=80;RO=2;RPL=16;RPP=37.7539;RPPR=7.35324;RPR=0;RUN=1;SAF=8;SAP=3.0103;SAR=8;SRF=1;SRP=3.0103;SRR=1;TYPE=snp\tGT:DP:AD:RO:QR:AO:QA:GL\t1:18:2,16:2:80:16:640:-50.3456,0" + } + } + + } +} From 19d1080f4cc7f7b63a3907ad9b4e4d72c9fe6d4f Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 16 Jan 2024 10:36:38 -0600 Subject: [PATCH 043/113] Adding PhyML test (similar to integration test). --- tests/modules/local/phyml/main.nf.test | 42 ++++++++++++++++++++++++++ 1 file changed, 42 insertions(+) create mode 100644 tests/modules/local/phyml/main.nf.test diff --git a/tests/modules/local/phyml/main.nf.test b/tests/modules/local/phyml/main.nf.test new file mode 100644 index 0000000..fb344c1 --- /dev/null +++ b/tests/modules/local/phyml/main.nf.test @@ -0,0 +1,42 @@ +nextflow_process { + + name "Test Process PHYML" + script "modules/local/phyml/main.nf" + process "PHYML" + + test("Should run without failures") { + + when { + params { + outdir = "results" + } + process { + """ + input[0] = file("$baseDir/tests/data/snvAlignment.phy") + """ + } + } + + then { + assert process.success + + with(process.out) { + def lines = path(phylogeneticTreeStats[0]).readLines() + + assert lines.contains(". Model of nucleotides substitution: \tGTR") + assert lines.contains(" - f(A)= 0.75000") + assert lines.contains(" - f(C)= 0.12500") + assert lines.contains(" - f(G)= 0.01000") + assert lines.contains(" - f(T)= 0.12500") + + lines = path(phylogeneticTree[0]).readLines() + + assert lines.join("\n").contains("SAMPLE1") + assert lines.join("\n").contains("SAMPLE2") + assert lines.join("\n").contains("SAMPLE3") + } + } + + } + +} From d46101f4fb1286968b452ac5141d4f2f1693f28c Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 16 Jan 2024 11:25:29 -0600 Subject: [PATCH 044/113] Adding missing file. --- tests/data/snvAlignment.phy | 6 ++++++ 1 file changed, 6 insertions(+) create mode 100644 tests/data/snvAlignment.phy diff --git a/tests/data/snvAlignment.phy b/tests/data/snvAlignment.phy new file mode 100644 index 0000000..c7510e4 --- /dev/null +++ b/tests/data/snvAlignment.phy @@ -0,0 +1,6 @@ + 4 2 + SAMPLE1 AA + SAMPLE2 TA + SAMPLE3 AC +reference AA + From 4c55e9c016a92e77fef0900b5867579f28e5bf7b Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 16 Jan 2024 12:03:00 -0600 Subject: [PATCH 045/113] Ignoring test data --- .github/workflows/linting.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index baa7f1b..26b66f8 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -22,7 +22,7 @@ jobs: run: npm install -g editorconfig-checker - name: Run ECLint check - run: editorconfig-checker -exclude LICENSE-Apachev2 README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE') + run: editorconfig-checker -exclude LICENSE-Apachev2 README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE|tests\data') Prettier: runs-on: ubuntu-latest From b09ff22fb103917efaad68ac3655506d7e8911e9 Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 16 Jan 2024 12:05:05 -0600 Subject: [PATCH 046/113] Lint ignore. --- .github/workflows/linting.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 26b66f8..00fdd61 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -22,7 +22,7 @@ jobs: run: npm install -g editorconfig-checker - name: Run ECLint check - run: editorconfig-checker -exclude LICENSE-Apachev2 README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE|tests\data') + run: editorconfig-checker -exclude LICENSE-Apachev2 README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE|tests\data\') Prettier: runs-on: ubuntu-latest From a4b24c29b7f97259bbfc5aa2a39231287167c7f4 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 17 Jan 2024 15:36:33 -0600 Subject: [PATCH 047/113] Removing GENERATE_LINE_1 and merging into the VERIFY process. --- modules/local/verifyingmapq/main.nf | 7 ++++++- workflows/snvphylnfc.nf | 8 +------- 2 files changed, 7 insertions(+), 8 deletions(-) diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index 8d10dab..f9148fd 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -14,13 +14,18 @@ process VERIFYING_MAP_Q { input: path(sorted_bams) - val(bam_line) output: path("mappingQuality.txt"), emit: mapping_quality path("versions.yml"), emit: versions script: + def bam_line = "" + + for (int i = 0; i Date: Wed, 17 Jan 2024 15:43:40 -0600 Subject: [PATCH 048/113] Removing mistakenly added file. --- .devcontainer/devcontainer.json | 28 ---------------------------- 1 file changed, 28 deletions(-) delete mode 100644 .devcontainer/devcontainer.json diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json deleted file mode 100644 index 4ecfbfe..0000000 --- a/.devcontainer/devcontainer.json +++ /dev/null @@ -1,28 +0,0 @@ -{ - "name": "nfcore", - "image": "nfcore/gitpod:latest", - "remoteUser": "gitpod", - "runArgs": ["--privileged"], - - // Configure tool-specific properties. - "customizations": { - // Configure properties specific to VS Code. - "vscode": { - // Set *default* container specific settings.json values on container create. - "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" - }, - - // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } -} From 847f914a6cece5f2d33ba21a3ef707bd8f0a7b01 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 17 Jan 2024 15:45:38 -0600 Subject: [PATCH 049/113] Removing unused module. --- modules/local/generateline1/main.nf | 31 ----------------------------- 1 file changed, 31 deletions(-) delete mode 100644 modules/local/generateline1/main.nf diff --git a/modules/local/generateline1/main.nf b/modules/local/generateline1/main.nf deleted file mode 100644 index d5e7bb5..0000000 --- a/modules/local/generateline1/main.nf +++ /dev/null @@ -1,31 +0,0 @@ -/* -This source file is adapted from a nf-core pipeline for SNVPhyl developed by -Jill Hagey as a work of the United States Government, which was distributed as -a work within the public domain under the Apache Software License version 2. - -This source file has been adapted to work within our pipeline. - -Please refer to the README for more information. -*/ -process GENERATE_LINE_1 { - label 'process_low' - container 'docker.io/python:3.9.17' - - input: - path(sorted_bams) - - output: - path("bam_line.txt"), emit: bam_lines_file - //path("versions.yml"), emit: versions - - shell: - ''' - #! /bin/bash -l - count=0 - for f in *_sorted.bam - do - ((count++)) - echo "--bam bam\$count=./$f " | tr -d "\n" >> bam_line.txt - done - ''' -} From 27ba0c6955df3ae231bf02ade9b65af8992364fb Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 17 Jan 2024 16:12:02 -0600 Subject: [PATCH 050/113] Removing GENERATE_LINE_2 process. --- modules/local/generateline2/main.nf | 30 -------------------------- modules/local/vcf2snvalignment/main.nf | 9 ++++++-- workflows/snvphylnfc.nf | 11 +--------- 3 files changed, 8 insertions(+), 42 deletions(-) delete mode 100644 modules/local/generateline2/main.nf diff --git a/modules/local/generateline2/main.nf b/modules/local/generateline2/main.nf deleted file mode 100644 index 8ac71af..0000000 --- a/modules/local/generateline2/main.nf +++ /dev/null @@ -1,30 +0,0 @@ -/* -This source file is adapted from a nf-core pipeline for SNVPhyl developed by -Jill Hagey as a work of the United States Government, which was distributed as -a work within the public domain under the Apache Software License version 2. - -This source file has been adapted to work within our pipeline. - -Please refer to the README for more information. -*/ -process GENERATE_LINE_2 { - label 'process_low' - container 'docker.io/python:3.9.17' - - input: - path(consolidated_bcf) - - output: - path("consolidation_line.txt"), emit: consolidation_line - //path("versions.yml"), emit: versions - - shell: - ''' - #! /bin/bash -l - for f in *_consolidated.bcf - do - fname=\$(basename $f _consolidated.bcf) - echo "--consolidate_vcf \$fname=$f " | tr -d "\n" >> consolidation_line.txt - done - ''' -} diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index 50d5902..0ef80c2 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -14,7 +14,6 @@ process VCF2SNV_ALIGNMENT { container = "staphb/snvphyl-tools:1.8.2" input: - val(consolidate_bcfs) path(bcf) path(new_invalid_positions) path(refgenome) @@ -27,8 +26,14 @@ process VCF2SNV_ALIGNMENT { path("versions.yml"), emit: versions script: + def bcf_line = "" + + for (int i = 0; i < bcf.size(); i++) { + bcf_line += "--consolidate_vcf v${i+1}=${bcf[i]} " + } + """ - vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus 4 --output-base snvalign --fasta ${refgenome} ${consolidate_bcfs} + vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus 4 --output-base snvalign --fasta ${refgenome} ${bcf_line} mv snvalign-positions.tsv snvTable.tsv mv snvalign-stats.csv vcf2core.tsv if [[ -f snvalign.phy ]]; then diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index ccc79ef..ed3e35b 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -57,7 +57,6 @@ include { BGZIP_MPILEUP_VCF } from '../modules/local/bgzipmpileupvcf/main' include { BCFTOOLS_CALL } from '../modules/local/bcftoolscall/main' include { CONSOLIDATE_BCFS } from '../modules/local/consolidatebcfs/main' include { CONSOLIDATE_FILTERED_DENSITY } from '../modules/local/consolidatefiltereddensity/main' -include { GENERATE_LINE_2 } from '../modules/local/generateline2/main' include { VCF2SNV_ALIGNMENT } from '../modules/local/vcf2snvalignment/main' include { FILTER_STATS } from '../modules/local/filterstats/main' include { PHYML } from '../modules/local/phyml/main' @@ -168,17 +167,9 @@ workflow SNVPHYL { ) ch_versions = ch_versions.mix(CONSOLIDATE_FILTERED_DENSITY.out.versions) - // Making string that looks like... this is needed for the next process - //--consolidate_vcf 2021JQ-00457-WAPHL-M5130-211029=2021JQ-00457-WAPHL-M5130-211029_consolidated.bcf --consolidate_vcf 2021JQ-00459-WAPHL-M5130-211029=2021JQ-00459-WAPHL-M5130-211029_consolidated.bcf --consolidate_vcf 2021JQ-00460-WAPHL-M5130-211029=2021JQ-00460-WAPHL-M5130-211029_consolidated.bcf - GENERATE_LINE_2( - CONSOLIDATE_BCFS.out.consolidated_bcfs.collect() - ) - //ch_versions = ch_versions.mix(GENERATE_LINE_2.out.versions) - - // Get line out of file we just made that has the --consolidate_vcf line... //13. consolidate variant calling files process takes 2 input channels as arguments VCF2SNV_ALIGNMENT( - GENERATE_LINE_2.out.consolidation_line.splitText(), CONSOLIDATE_BCFS.out.consolidated_bcfs.collect(), CONSOLIDATE_FILTERED_DENSITY.out.new_invalid_positions, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() + CONSOLIDATE_BCFS.out.consolidated_bcfs.collect(), CONSOLIDATE_FILTERED_DENSITY.out.new_invalid_positions, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() ) ch_versions = ch_versions.mix(VCF2SNV_ALIGNMENT.out.versions) From 126e194f758eb46c24f267114240ff035928faee Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 17 Jan 2024 16:13:15 -0600 Subject: [PATCH 051/113] Removing unused modules. --- modules/local/generatesamplejson/main.nf | 49 ------------------------ modules/local/generatesummary/main.nf | 38 ------------------ modules/local/iridanextoutput/main.nf | 31 --------------- modules/local/simplifyiridajson/main.nf | 33 ---------------- 4 files changed, 151 deletions(-) delete mode 100644 modules/local/generatesamplejson/main.nf delete mode 100644 modules/local/generatesummary/main.nf delete mode 100644 modules/local/iridanextoutput/main.nf delete mode 100644 modules/local/simplifyiridajson/main.nf diff --git a/modules/local/generatesamplejson/main.nf b/modules/local/generatesamplejson/main.nf deleted file mode 100644 index f3b5cd3..0000000 --- a/modules/local/generatesamplejson/main.nf +++ /dev/null @@ -1,49 +0,0 @@ -process GENERATE_SAMPLE_JSON { - tag "$meta.id" - label 'process_single' - - container 'docker.io/python:3.9.17' - - input: - tuple val(meta), path(reads), path(assembly) - - output: - tuple val(meta), path("*.json.gz"), emit: json - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def assembly_path = ["${task.assembly_directory_name}", "${assembly}"].join(File.separator) - """ - cat <<-EOF > "${meta.id}.json" - { - "files": { - "samples": { - "${meta.id}": [ - { - "path": "${assembly_path}" - } - ] - } - }, - "metadata": { - "samples": { - "${meta.id}": { - "reads": ["${reads[0]}", "${reads[1]}"] - } - } - } - } - EOF - gzip ${meta.id}.json - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - generatesamplejson : 0.1.0 - END_VERSIONS - """ -} diff --git a/modules/local/generatesummary/main.nf b/modules/local/generatesummary/main.nf deleted file mode 100644 index dedc31b..0000000 --- a/modules/local/generatesummary/main.nf +++ /dev/null @@ -1,38 +0,0 @@ -process GENERATE_SUMMARY { - label 'process_single' - container 'docker.io/python:3.9.17' - - input: - val summaries - - output: - path("summary.txt.gz"), emit: summary - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def sorted_summaries = summaries.sort{ it[0].id } - - // Generate summary text: - def summary_text = "SNVPhyl Pipeline Summary\n\nSUCCESS!\n" - - // TODO: Consider the possibility of code injection. - // Should probably be moved to file processing through Python. - for (summary in sorted_summaries) { - summary_text += "\n${summary[0].id}:\n" - summary_text += " reads.1: ${summary[1][0]}\n" - summary_text += " reads.2: ${summary[1][1]}\n" - summary_text += " assembly: ${summary[2]}\n" - } - - version_text = "\"${task.process}\":\n generatesummary : 0.1.0" - - """ - echo "${summary_text}" > summary.txt - gzip -n summary.txt - echo "${version_text}" > versions.yml - """ -} diff --git a/modules/local/iridanextoutput/main.nf b/modules/local/iridanextoutput/main.nf deleted file mode 100644 index 92595ee..0000000 --- a/modules/local/iridanextoutput/main.nf +++ /dev/null @@ -1,31 +0,0 @@ -process IRIDA_NEXT_OUTPUT { - label 'process_single' - - container 'docker.io/python:3.9.17' - - input: - path(samples_data) - - output: - path("iridanext.output.json.gz"), emit: output_json - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def samples_data_dir = "samples_data" - """ - irida-next-output.py \\ - $args \\ - --summary-file ${task.summary_directory_name}/summary.txt.gz \\ - --json-output iridanext.output.json.gz \\ - ${samples_data} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - iridanextoutput : 0.1.0 - END_VERSIONS - """ -} diff --git a/modules/local/simplifyiridajson/main.nf b/modules/local/simplifyiridajson/main.nf deleted file mode 100644 index e2e7352..0000000 --- a/modules/local/simplifyiridajson/main.nf +++ /dev/null @@ -1,33 +0,0 @@ -process SIMPLIFY_IRIDA_JSON { - tag "$meta.id" - label 'process_single' - - container 'docker.io/python:3.9.17' - - input: - tuple val(meta), path(json) - - output: - tuple val(meta), path("*.simple.json.gz") , emit: simple_json - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - simplify_irida_json.py \\ - $args \\ - --json-output ${meta.id}.simple.json \\ - ${json} - - gzip ${meta.id}.simple.json - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - simplifyiridajson : 0.1.0 - END_VERSIONS - """ -} From 5b343cf61a6424fbf1a1bf5b9d99e1e35dc91d5d Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 17 Jan 2024 16:41:43 -0600 Subject: [PATCH 052/113] Making changes to propagate sample name correctly. --- modules/local/consolidatebcfs/main.nf | 10 +++++----- modules/local/vcf2snvalignment/main.nf | 6 +++--- workflows/snvphylnfc.nf | 2 +- 3 files changed, 9 insertions(+), 9 deletions(-) diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index 737e80a..1d64f18 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -16,11 +16,11 @@ process CONSOLIDATE_BCFS { tuple val(meta), path(mpileup_bcf), path(freebayes_filtered_bcf), path(freebayes_filtered_csi) output: - path( "${meta.id}_consolidated.bcf" ), emit: consolidated_bcfs - path( "${meta.id}_consolidated.vcf" ), emit: consolidated_vcfs - path( "${meta.id}_consolidated.bcf.csi" ), emit: consolidated_bcf_index - path( "${meta.id}_filtered_density.txt" ), emit: filtered_densities - path("versions.yml"), emit: versions + tuple val(meta), path( "${meta.id}_consolidated.bcf" ), emit: consolidated_bcfs + path( "${meta.id}_consolidated.vcf" ) , emit: consolidated_vcfs + path( "${meta.id}_consolidated.bcf.csi" ) , emit: consolidated_bcf_index + path( "${meta.id}_filtered_density.txt" ) , emit: filtered_densities + path("versions.yml") , emit: versions script: def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index 0ef80c2..f3c8a1d 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -14,7 +14,7 @@ process VCF2SNV_ALIGNMENT { container = "staphb/snvphyl-tools:1.8.2" input: - path(bcf) + val(bcfs) path(new_invalid_positions) path(refgenome) path(consolidated_bcf_index) @@ -28,8 +28,8 @@ process VCF2SNV_ALIGNMENT { script: def bcf_line = "" - for (int i = 0; i < bcf.size(); i++) { - bcf_line += "--consolidate_vcf v${i+1}=${bcf[i]} " + for (int i = 0; i < bcfs.size(); i++) { + bcf_line += "--consolidate_vcf ${bcfs[i][0].id}=${bcfs[i][1]} " } """ diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index ed3e35b..7ce650e 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -169,7 +169,7 @@ workflow SNVPHYL { //13. consolidate variant calling files process takes 2 input channels as arguments VCF2SNV_ALIGNMENT( - CONSOLIDATE_BCFS.out.consolidated_bcfs.collect(), CONSOLIDATE_FILTERED_DENSITY.out.new_invalid_positions, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() + CONSOLIDATE_BCFS.out.consolidated_bcfs.toList(), CONSOLIDATE_FILTERED_DENSITY.out.new_invalid_positions, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() ) ch_versions = ch_versions.mix(VCF2SNV_ALIGNMENT.out.versions) From a4aa2329413aa7b422e537b61bb66bd6da98d7f8 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 19 Jan 2024 10:34:31 -0600 Subject: [PATCH 053/113] Adding test for smalt map. --- tests/data/SAMPLE1_1.fastq | 36 ++++++++++++++++++++++ tests/data/SAMPLE1_2.fastq | 36 ++++++++++++++++++++++ tests/data/reference.fasta.fai | 1 + tests/data/reference.sma | Bin 0 -> 378 bytes tests/data/reference.smi | Bin 0 -> 1728 bytes tests/modules/local/smaltmap/main.nf.test | 35 +++++++++++++++++++++ 6 files changed, 108 insertions(+) create mode 100644 tests/data/SAMPLE1_1.fastq create mode 100644 tests/data/SAMPLE1_2.fastq create mode 100644 tests/data/reference.fasta.fai create mode 100644 tests/data/reference.sma create mode 100644 tests/data/reference.smi create mode 100644 tests/modules/local/smaltmap/main.nf.test diff --git a/tests/data/SAMPLE1_1.fastq b/tests/data/SAMPLE1_1.fastq new file mode 100644 index 0000000..ce5376e --- /dev/null +++ b/tests/data/SAMPLE1_1.fastq @@ -0,0 +1,36 @@ +@1 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@2 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@3 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@4 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@5 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@6 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@7 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@8 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@9 +tccacaagccattgtgtgtaattaaccactaattgtgtataagtttaaact ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII diff --git a/tests/data/SAMPLE1_2.fastq b/tests/data/SAMPLE1_2.fastq new file mode 100644 index 0000000..8d07d68 --- /dev/null +++ b/tests/data/SAMPLE1_2.fastq @@ -0,0 +1,36 @@ +@1 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@2 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@3 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@4 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@5 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@6 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@7 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@8 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII +@9 +agtttaaacttatacacaattagtggttaattacacacaatggcttgtgga ++ +IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII diff --git a/tests/data/reference.fasta.fai b/tests/data/reference.fasta.fai new file mode 100644 index 0000000..628e0fb --- /dev/null +++ b/tests/data/reference.fasta.fai @@ -0,0 +1 @@ +reference 700 11 70 71 diff --git a/tests/data/reference.sma b/tests/data/reference.sma new file mode 100644 index 0000000000000000000000000000000000000000..baf17284a32849c4aa32d5419525be677b681219 GIT binary patch literal 378 zcmZ{eK}*7L7{;If|DCa>gE1wxc9|D3a3B$@V+w(U`v7%G$%Vq^LBGH%395^9h@gvy z@H~d>)IstKSSMp&rkP!P;K%d6ye~Y@uvi@G4~P8=04zmF5mWxt`e!X^V|eGA;m_zG=O`bMB_<|Q8UPuhi3CX;#Hfj8E;8a0zqa1@Qa!WDl{f3Q@8^B) z`;Apq#mdiZ>Azx$7!gq@hX4DN;ul2_gEs;HHvco0`R};$Itzh+R|1Q{0nP#!R$2zj z3*1;&6g-A?Wx;B!w5DJ!*7fi>Y=9@=N!SFN;VF0;wm}_)pcz`=SqMWr?0}un0TI{( zFTy@J00*HHy5JBTDL9JtbvRxyfR&zU`XXypriyt3wUzP&*i0}Up z@cMioUh@%Xf_1=W;(I>;J;1%I0`3iGPzij_Rwx2K?|$GrZwKy&7utaDTn-C>`_>ON zumtMiWiY^baE{yy?rjjBgT1f|UI9OF?>NWjp%J(@4+68qIW@paXo20Z1-1g`Pz!y) zIdXQ@5CA8<0nFke=mhRN_xKs$>|O9GJPa=YXUFVt{>PvKx}gg=A7+R%_rOL70dsj6 zUW1o_S$Pynff*Se@<{4@TE**^toM>kxH?p2lcpVU+0z)8-6auS;cwBV8zbg+MMTrp zadi)Vr5WoFGt_Wa?&t^d*QisRjCxh*I9V`pU5BH2N2)C-lH_G%_ zL_VDvwtkbF`c5Jz#@}qU*gN#D>m!}HdQC&v$S;2n$}hf;t)DWBHG{zq)UFZNqPWSY zPs!z5VwPXA^!nV_>g)+xwkM$z756os9g~^q4&zzq(mr=kBl&G+(wbA3_q1G?{ZUd^ zMUqK}rbg>D6!=`~9p%zJJ0nX%Ir%m7u9bHO<=*94Nd!dATom`SO-;S(``0s;`cLZY zsWalrOvtj$J`;%M%s$&AW-Mb(<)f0&DYLE^6B=^Mx0$Vy{Op!qO#Z2{lX<;qpBMYR zGxEjVq*S(^Hly9+I?6C$J}@850`thcEd_Ruy=E7gduE;8We(Y6c8lF%f0;GriMeDRE1?S5hgGl| zn9H@mo_m2kXK&br8rT5rhY$R~?DC9YPcX*+&b@zo#r(4)>^%F$vxnVfw?ohXjnD+l z`*vVAc~*phXGj~g13R!2b^*KF0TJLiz}~a->>kg$y}%B#NBe>2$N}IvaS+(mPUwO| g&<%&72ado|I0n7IKC Date: Fri, 19 Jan 2024 13:28:15 -0600 Subject: [PATCH 054/113] Replacing consolidatefiltereddensity with CAT_CAT. --- modules.json | 5 + .../local/consolidatefiltereddensity/main.nf | 33 ---- modules/nf-core/cat/cat/environment.yml | 7 + modules/nf-core/cat/cat/main.nf | 79 ++++++++ modules/nf-core/cat/cat/meta.yml | 36 ++++ modules/nf-core/cat/cat/tests/main.nf.test | 177 ++++++++++++++++++ .../nf-core/cat/cat/tests/main.nf.test.snap | 145 ++++++++++++++ .../cat/tests/nextflow_unzipped_zipped.config | 6 + .../cat/tests/nextflow_zipped_unzipped.config | 8 + modules/nf-core/cat/cat/tests/tags.yml | 2 + workflows/snvphylnfc.nf | 15 +- 11 files changed, 474 insertions(+), 39 deletions(-) delete mode 100644 modules/local/consolidatefiltereddensity/main.nf create mode 100644 modules/nf-core/cat/cat/environment.yml create mode 100644 modules/nf-core/cat/cat/main.nf create mode 100644 modules/nf-core/cat/cat/meta.yml create mode 100644 modules/nf-core/cat/cat/tests/main.nf.test create mode 100644 modules/nf-core/cat/cat/tests/main.nf.test.snap create mode 100644 modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config create mode 100644 modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config create mode 100644 modules/nf-core/cat/cat/tests/tags.yml diff --git a/modules.json b/modules.json index c531eab..fdd3421 100644 --- a/modules.json +++ b/modules.json @@ -5,6 +5,11 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { + "cat/cat": { + "branch": "master", + "git_sha": "81f27e75847087865299cc46605deb3b09b4e0a2", + "installed_by": ["modules"] + }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "1526dc37227a1101bdca25339337362e187a6b3b", diff --git a/modules/local/consolidatefiltereddensity/main.nf b/modules/local/consolidatefiltereddensity/main.nf deleted file mode 100644 index 509c131..0000000 --- a/modules/local/consolidatefiltereddensity/main.nf +++ /dev/null @@ -1,33 +0,0 @@ -/* -This source file is adapted from a nf-core pipeline for SNVPhyl developed by -Jill Hagey as a work of the United States Government, which was distributed as -a work within the public domain under the Apache Software License version 2. - -This source file has been adapted to work within our pipeline. - -Please refer to the README for more information. -*/ -process CONSOLIDATE_FILTERED_DENSITY { - label 'process_low' - container = 'docker.io/python:3.9.17' - - input: - path(filtered_densities) - path(invalid_positions) - - output: - path('filtered_density_all.txt'), emit: filtered_densities - path('new_invalid_positions.bed'), emit: new_invalid_positions - path("versions.yml"), emit: versions - - script: - """ - find ./ -name '*_filtered_density.txt' -exec cat {} + > filtered_density_all.txt - catWrapper.py new_invalid_positions.bed filtered_density_all.txt ${invalid_positions} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/cat/cat/environment.yml b/modules/nf-core/cat/cat/environment.yml new file mode 100644 index 0000000..17a04ef --- /dev/null +++ b/modules/nf-core/cat/cat/environment.yml @@ -0,0 +1,7 @@ +name: cat_cat +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::pigz=2.3.4 diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf new file mode 100644 index 0000000..adbdbd7 --- /dev/null +++ b/modules/nf-core/cat/cat/main.nf @@ -0,0 +1,79 @@ +process CAT_CAT { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/pigz:2.3.4' : + 'biocontainers/pigz:2.3.4' }" + + input: + tuple val(meta), path(files_in) + + output: + tuple val(meta), path("${prefix}"), emit: file_out + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def file_list = files_in.collect { it.toString() } + + // choose appropriate concatenation tool depending on input and output format + + // | input | output | command1 | command2 | + // |-----------|------------|----------|----------| + // | gzipped | gzipped | cat | | + // | ungzipped | ungzipped | cat | | + // | gzipped | ungzipped | zcat | | + // | ungzipped | gzipped | cat | pigz | + + // Use input file ending as default + prefix = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}" + out_zip = prefix.endsWith('.gz') + in_zip = file_list[0].endsWith('.gz') + command1 = (in_zip && !out_zip) ? 'zcat' : 'cat' + command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : '' + if(file_list.contains(prefix.trim())) { + error "The name of the input file can't be the same as for the output prefix in the " + + "module CAT_CAT (currently `$prefix`). Please choose a different one." + } + """ + $command1 \\ + $args \\ + ${file_list.join(' ')} \\ + $command2 \\ + > ${prefix} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) + END_VERSIONS + """ + + stub: + def file_list = files_in.collect { it.toString() } + prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" + if(file_list.contains(prefix.trim())) { + error "The name of the input file can't be the same as for the output prefix in the " + + "module CAT_CAT (currently `$prefix`). Please choose a different one." + } + """ + touch $prefix + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) + END_VERSIONS + """ +} + +// for .gz files also include the second to last extension if it is present. E.g., .fasta.gz +def getFileSuffix(filename) { + def match = filename =~ /^.*?((\.\w{1,5})?(\.\w{1,5}\.gz$))/ + return match ? match[0][1] : filename.substring(filename.lastIndexOf('.')) +} + diff --git a/modules/nf-core/cat/cat/meta.yml b/modules/nf-core/cat/cat/meta.yml new file mode 100644 index 0000000..00a8db0 --- /dev/null +++ b/modules/nf-core/cat/cat/meta.yml @@ -0,0 +1,36 @@ +name: cat_cat +description: A module for concatenation of gzipped or uncompressed files +keywords: + - concatenate + - gzip + - cat +tools: + - cat: + description: Just concatenation + documentation: https://man7.org/linux/man-pages/man1/cat.1.html + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - files_in: + type: file + description: List of compressed / uncompressed files + pattern: "*" +output: + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - file_out: + type: file + description: Concatenated file. Will be gzipped if file_out ends with ".gz" + pattern: "${file_out}" +authors: + - "@erikrikarddaniel" + - "@FriederikeHanssen" +maintainers: + - "@erikrikarddaniel" + - "@FriederikeHanssen" diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test new file mode 100644 index 0000000..aaae04f --- /dev/null +++ b/modules/nf-core/cat/cat/tests/main.nf.test @@ -0,0 +1,177 @@ +nextflow_process { + + name "Test Process CAT_CAT" + script "../main.nf" + process "CAT_CAT" + tag "modules" + tag "modules_nfcore" + tag "cat" + tag "cat/cat" + + test("test_cat_name_conflict") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'genome', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") } + ) + } + } + + test("test_cat_unzipped_unzipped") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + + test("test_cat_zipped_zipped") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_cat_zipped_unzipped") { + config './nextflow_zipped_unzipped.config' + + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("test_cat_unzipped_zipped") { + config './nextflow_unzipped_zipped.config' + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_cat_one_file_unzipped_zipped") { + config './nextflow_unzipped_zipped.config' + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(lines[0..5]).match("test_cat_one_file_unzipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_one_file_unzipped_zipped_size")} + ) + } + } +} + diff --git a/modules/nf-core/cat/cat/tests/main.nf.test.snap b/modules/nf-core/cat/cat/tests/main.nf.test.snap new file mode 100644 index 0000000..0c9bfe8 --- /dev/null +++ b/modules/nf-core/cat/cat/tests/main.nf.test.snap @@ -0,0 +1,145 @@ +{ + "test_cat_unzipped_unzipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2023-10-16T14:32:18.500464399" + }, + "test_cat_zipped_unzipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2023-10-16T14:32:49.642741302" + }, + "test_cat_zipped_zipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2024-01-12T14:02:02.999254641" + }, + "test_cat_one_file_unzipped_zipped_lines": { + "content": [ + [ + ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", + "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", + "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", + "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", + "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", + "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" + ] + ], + "timestamp": "2023-10-16T14:33:21.39642399" + }, + "test_cat_unzipped_zipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2024-01-12T14:08:26.948048418" + }, + "test_cat_one_file_unzipped_zipped_size": { + "content": [ + 374 + ], + "timestamp": "2024-01-12T14:10:22.445700266" + } +} diff --git a/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config b/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config new file mode 100644 index 0000000..ec26b0f --- /dev/null +++ b/modules/nf-core/cat/cat/tests/nextflow_unzipped_zipped.config @@ -0,0 +1,6 @@ + +process { + withName: CAT_CAT { + ext.prefix = 'cat.txt.gz' + } +} diff --git a/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config b/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config new file mode 100644 index 0000000..fbc7978 --- /dev/null +++ b/modules/nf-core/cat/cat/tests/nextflow_zipped_unzipped.config @@ -0,0 +1,8 @@ + +process { + + withName: CAT_CAT { + ext.prefix = 'cat.txt' + } + +} diff --git a/modules/nf-core/cat/cat/tests/tags.yml b/modules/nf-core/cat/cat/tests/tags.yml new file mode 100644 index 0000000..37b578f --- /dev/null +++ b/modules/nf-core/cat/cat/tests/tags.yml @@ -0,0 +1,2 @@ +cat/cat: + - modules/nf-core/cat/cat/** diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 7ce650e..1ca44ad 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -56,7 +56,6 @@ include { MPILEUP } from '../modules/local/mpileup/main' include { BGZIP_MPILEUP_VCF } from '../modules/local/bgzipmpileupvcf/main' include { BCFTOOLS_CALL } from '../modules/local/bcftoolscall/main' include { CONSOLIDATE_BCFS } from '../modules/local/consolidatebcfs/main' -include { CONSOLIDATE_FILTERED_DENSITY } from '../modules/local/consolidatefiltereddensity/main' include { VCF2SNV_ALIGNMENT } from '../modules/local/vcf2snvalignment/main' include { FILTER_STATS } from '../modules/local/filterstats/main' include { PHYML } from '../modules/local/phyml/main' @@ -72,6 +71,7 @@ include { MAKE_SNV } from '../modules/local/makesnv/main' // MODULE: Installed directly from nf-core/modules // include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { CAT_CAT } from '../modules/nf-core/cat/cat/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -162,14 +162,17 @@ workflow SNVPHYL { ch_versions = ch_versions.mix(CONSOLIDATE_BCFS.out.versions) // Concat filtered densities to make new invalid_postions - CONSOLIDATE_FILTERED_DENSITY( - CONSOLIDATE_BCFS.out.filtered_densities.collect(), FIND_REPEATS.out.repeats_bed_file - ) - ch_versions = ch_versions.mix(CONSOLIDATE_FILTERED_DENSITY.out.versions) + invalid_positions = CONSOLIDATE_BCFS.out.filtered_densities + .concat(FIND_REPEATS.out.repeats_bed_file) + .collect().map{it -> [[id: 'invalid_positions'], it]} + + CAT_CAT(invalid_positions) + + invalid_positions_file = CAT_CAT.out.file_out.map{meta, filepath -> filepath} //13. consolidate variant calling files process takes 2 input channels as arguments VCF2SNV_ALIGNMENT( - CONSOLIDATE_BCFS.out.consolidated_bcfs.toList(), CONSOLIDATE_FILTERED_DENSITY.out.new_invalid_positions, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() + CONSOLIDATE_BCFS.out.consolidated_bcfs.toList(), invalid_positions_file, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() ) ch_versions = ch_versions.mix(VCF2SNV_ALIGNMENT.out.versions) From c16fb9d295845c8270abc2e4d8fde850daf5031c Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 22 Jan 2024 12:37:53 -0600 Subject: [PATCH 055/113] Removing unused catWrapper.py --- bin/catWrapper.py | 50 ----------------------------------------------- 1 file changed, 50 deletions(-) delete mode 100755 bin/catWrapper.py diff --git a/bin/catWrapper.py b/bin/catWrapper.py deleted file mode 100755 index 3274c1f..0000000 --- a/bin/catWrapper.py +++ /dev/null @@ -1,50 +0,0 @@ -#!/usr/bin/env python -""" -This source file was developed by Guruprasad Ananda and was included in a -nf-core pipeline for SNVPhyl developed by Jill Hagey as a work of the United -States Government, which was distributed as a work within the public domain -under the Apache Software License version 2. - -This source file has been adapted to work within our pipeline. - -Please refer to the README for more information. -""" - -import sys, os - - -def stop_err(msg): - sys.stderr.write(msg) - sys.exit() - - -def main(): - outfile = sys.argv[1] - infile = sys.argv[2] - - try: - fout = open(sys.argv[1], "w") - except: - stop_err("Output file cannot be opened for writing.") - - try: - fin = open(sys.argv[2], "r") - except: - stop_err("Input file cannot be opened for reading.") - - if len(sys.argv) < 4: - os.system("cp %s %s" % (infile, outfile)) - sys.exit() - - cmdline = "cat %s " % (infile) - for inp in sys.argv[3:]: - cmdline = cmdline + inp + " " - cmdline = cmdline + ">" + outfile - try: - os.system(cmdline) - except: - stop_err("Error encountered with cat.") - - -if __name__ == "__main__": - main() From 5f1cf2c9af5e02908d64eb97d3c104647eeceff7 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 22 Jan 2024 12:39:04 -0600 Subject: [PATCH 056/113] Set cores to task.cpus. --- modules/local/smaltmap/main.nf | 2 +- modules/local/vcf2snvalignment/main.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/smaltmap/main.nf b/modules/local/smaltmap/main.nf index 7b07c88..438424a 100644 --- a/modules/local/smaltmap/main.nf +++ b/modules/local/smaltmap/main.nf @@ -24,7 +24,7 @@ process SMALT_MAP { script: def prefix = task.ext.prefix ?: "${meta.id}" - def smalt_map_command = reads[1] ? "smalt map -f bam -n 4 -l pe -i 1000 -j 20 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]} ${reads[1]}": "smalt map -f bam -n 4 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]}" + def smalt_map_command = reads[1] ? "smalt map -f bam -n ${task.cpus} -l pe -i 1000 -j 20 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]} ${reads[1]}": "smalt map -f bam -n 4 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]}" """ REFNAME=\$(basename ${ref_sma} .sma) ${smalt_map_command} diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index f3c8a1d..d0d2102 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -33,7 +33,7 @@ process VCF2SNV_ALIGNMENT { } """ - vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus 4 --output-base snvalign --fasta ${refgenome} ${bcf_line} + vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus ${task.cpus} --output-base snvalign --fasta ${refgenome} ${bcf_line} mv snvalign-positions.tsv snvTable.tsv mv snvalign-stats.csv vcf2core.tsv if [[ -f snvalign.phy ]]; then From 13a0ab22a73ac83e958b7f6071ff0056321b4f90 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 22 Jan 2024 12:45:44 -0600 Subject: [PATCH 057/113] 2 sed commands into 1 global. --- modules/local/vcf2snvalignment/main.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index d0d2102..dc1ee75 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -38,8 +38,7 @@ process VCF2SNV_ALIGNMENT { mv snvalign-stats.csv vcf2core.tsv if [[ -f snvalign.phy ]]; then mv snvalign.phy snvAlignment.phy - sed -i "s/'//" snvAlignment.phy - sed -i "s/'//" snvAlignment.phy + sed -i "s/'//g" snvAlignment.phy else touch snvAlignment.phy fi From 6969a37e96983b1f68702c4264f616cb15b84208 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 22 Jan 2024 12:55:57 -0600 Subject: [PATCH 058/113] Changing bam1, bam2, etc. to file names. --- modules/local/verifyingmapq/main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index f9148fd..ac843aa 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -22,8 +22,8 @@ process VERIFYING_MAP_Q { script: def bam_line = "" - for (int i = 0; i Date: Tue, 23 Jan 2024 09:23:19 -0600 Subject: [PATCH 059/113] Variable CPUs. --- modules/local/smaltmap/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/smaltmap/main.nf b/modules/local/smaltmap/main.nf index 438424a..1f5e2fb 100644 --- a/modules/local/smaltmap/main.nf +++ b/modules/local/smaltmap/main.nf @@ -24,7 +24,7 @@ process SMALT_MAP { script: def prefix = task.ext.prefix ?: "${meta.id}" - def smalt_map_command = reads[1] ? "smalt map -f bam -n ${task.cpus} -l pe -i 1000 -j 20 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]} ${reads[1]}": "smalt map -f bam -n 4 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]}" + def smalt_map_command = reads[1] ? "smalt map -f bam -n ${task.cpus} -l pe -i 1000 -j 20 -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]} ${reads[1]}": "smalt map -f bam -n ${task.cpus} -r 1 -y 0.5 -o ${prefix}.bam \${REFNAME} ${reads[0]}" """ REFNAME=\$(basename ${ref_sma} .sma) ${smalt_map_command} From 41211dbcf0cb688733429a0ee5f5f93886244aee Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 23 Jan 2024 10:47:05 -0600 Subject: [PATCH 060/113] Removing VS Code file. --- .devcontainer/devcontainer.json | 28 ---------------------------- 1 file changed, 28 deletions(-) delete mode 100644 .devcontainer/devcontainer.json diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json deleted file mode 100644 index 4ecfbfe..0000000 --- a/.devcontainer/devcontainer.json +++ /dev/null @@ -1,28 +0,0 @@ -{ - "name": "nfcore", - "image": "nfcore/gitpod:latest", - "remoteUser": "gitpod", - "runArgs": ["--privileged"], - - // Configure tool-specific properties. - "customizations": { - // Configure properties specific to VS Code. - "vscode": { - // Set *default* container specific settings.json values on container create. - "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" - }, - - // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } -} From 59a15e1860e0ee1b6ea92b5373d47a163564b7f4 Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 23 Jan 2024 13:07:16 -0600 Subject: [PATCH 061/113] Adding iridanext nextflow plugin. --- conf/iridanext.config | 18 ++++++++++++++++++ nextflow.config | 2 ++ 2 files changed, 20 insertions(+) create mode 100644 conf/iridanext.config diff --git a/conf/iridanext.config b/conf/iridanext.config new file mode 100644 index 0000000..8af7050 --- /dev/null +++ b/conf/iridanext.config @@ -0,0 +1,18 @@ +iridanext { + enabled = true + output { + path = "${params.outdir}/iridanext.output.json.gz" + overwrite = true + files { + global = [ + "**/filter/filterStats.txt", + "**/verifying/mappingQuality.txt", + "**/phyml/phylogeneticTree.newick", + "**/phyml/phylogeneticTreeStats.txt", + "**/vcf2snv/snvAlignment.phy", + "**/make/snvMatrix.tsv", + "**/vcf2snv/snvTable.tsv", + "**/vcf2snv/vcf2core.tsv",] + } + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 10eab56..de2e4bf 100644 --- a/nextflow.config +++ b/nextflow.config @@ -57,6 +57,7 @@ params { // Load base.config by default for all pipelines includeConfig 'conf/base.config' +includeConfig 'conf/iridanext.config' profiles { debug { @@ -163,6 +164,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-iridanext' // Output for IRIDA Next } // Export these variables to prevent local Python/R libraries from conflicting with those in the container From ea6f6ee1853b4baca6e48e3be825a861bbfba3f1 Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 23 Jan 2024 13:29:32 -0600 Subject: [PATCH 062/113] Adding simple integration tests. --- tests/workflows/snvphylnfc.nf.test | 13 ++++++++++++- 1 file changed, 12 insertions(+), 1 deletion(-) diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index 3531c4e..9d22093 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -50,7 +50,18 @@ nextflow_workflow { assert lines.contains("SAMPLE1\t1\t1\t0\t0\t") assert lines.contains("SAMPLE2\t0\t2\t1\t1\t") assert lines.contains("SAMPLE3\t2\t0\t1\t1\t") - + + // check IRIDA Next JSON file + lines = path("$launchDir/results/iridanext.output.json.gz").linesGzip.join("\n") + + assert lines.contains("\"path\": \"make/snvMatrix.tsv\"") + assert lines.contains("\"path\": \"filter/filterStats.txt\"") + assert lines.contains("\"path\": \"phyml/phylogeneticTreeStats.txt\"") + assert lines.contains("\"path\": \"phyml/phylogeneticTree.newick\"") + assert lines.contains("\"path\": \"vcf2snv/snvTable.tsv\"") + assert lines.contains("\"path\": \"vcf2snv/vcf2core.tsv\"") + assert lines.contains("\"path\": \"vcf2snv/snvAlignment.phy\"") + assert lines.contains("\"path\": \"verifying/mappingQuality.txt\"") } } From 19107af7904e5eb658cc93457fd2da13fb836f33 Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 23 Jan 2024 13:31:49 -0600 Subject: [PATCH 063/113] Newline at end of file. --- conf/iridanext.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/iridanext.config b/conf/iridanext.config index 8af7050..680584b 100644 --- a/conf/iridanext.config +++ b/conf/iridanext.config @@ -15,4 +15,4 @@ iridanext { "**/vcf2snv/vcf2core.tsv",] } } -} \ No newline at end of file +} From ac4837015962a32a190aa0bd05e80ff9ab0c44a1 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 24 Jan 2024 06:40:48 -0600 Subject: [PATCH 064/113] val to path --- modules/local/vcf2snvalignment/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index dc1ee75..d09c051 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -14,7 +14,7 @@ process VCF2SNV_ALIGNMENT { container = "staphb/snvphyl-tools:1.8.2" input: - val(bcfs) + path(bcfs) path(new_invalid_positions) path(refgenome) path(consolidated_bcf_index) From 17f7cc3a7a1b258a1eddd5684dd2be21b168b83d Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 24 Jan 2024 08:51:06 -0600 Subject: [PATCH 065/113] metas and paths into two separate inputs --- modules/local/vcf2snvalignment/main.nf | 7 ++++--- workflows/snvphylnfc.nf | 13 +++++++++++-- 2 files changed, 15 insertions(+), 5 deletions(-) diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index d09c051..2bf9c11 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -14,7 +14,8 @@ process VCF2SNV_ALIGNMENT { container = "staphb/snvphyl-tools:1.8.2" input: - path(bcfs) + val(bcf_metas) // list of metas + path(bcf_paths) // list of paths path(new_invalid_positions) path(refgenome) path(consolidated_bcf_index) @@ -28,8 +29,8 @@ process VCF2SNV_ALIGNMENT { script: def bcf_line = "" - for (int i = 0; i < bcfs.size(); i++) { - bcf_line += "--consolidate_vcf ${bcfs[i][0].id}=${bcfs[i][1]} " + for (int i = 0; i < bcf_paths.size(); i++) { + bcf_line += "--consolidate_vcf ${bcf_metas[i].id}=${bcf_paths[i]} " } """ diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 1ca44ad..0fa9cd1 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -169,10 +169,19 @@ workflow SNVPHYL { CAT_CAT(invalid_positions) invalid_positions_file = CAT_CAT.out.file_out.map{meta, filepath -> filepath} + consolidated_bcfs = CONSOLIDATE_BCFS.out.consolidated_bcfs.toSortedList{a, b -> a[0].id <=> b[0].id} - //13. consolidate variant calling files process takes 2 input channels as arguments + // consolidated_bcfs is a list of [meta, filepath] tuples + // .map{} iterates onces, because there is only one item (one list) in the channel + // .collect{} transforms each item in the list with the operation + // which ultimately unzips the tuples + consolidated_bcfs_metas = consolidated_bcfs.map{ it.collect { it[0] } } + consolidated_bcfs_paths = consolidated_bcfs.map{ it.collect { it[1] } } + + //13. consolidate variant calling files VCF2SNV_ALIGNMENT( - CONSOLIDATE_BCFS.out.consolidated_bcfs.toList(), invalid_positions_file, params.refgenome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() + consolidated_bcfs_metas, consolidated_bcfs_paths, invalid_positions_file, params.refgenome, + CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() ) ch_versions = ch_versions.mix(VCF2SNV_ALIGNMENT.out.versions) From 3cba6ac259aeabf31eff412a5c02e2b4214cd57f Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 24 Jan 2024 10:03:04 -0600 Subject: [PATCH 066/113] map to collect --- modules/local/vcf2snvalignment/main.nf | 4 ++-- workflows/snvphylnfc.nf | 4 ++-- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index 2bf9c11..f4ccad6 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -14,8 +14,8 @@ process VCF2SNV_ALIGNMENT { container = "staphb/snvphyl-tools:1.8.2" input: - val(bcf_metas) // list of metas - path(bcf_paths) // list of paths + val(bcf_metas) // list of metas + path(bcf_paths) // list of paths path(new_invalid_positions) path(refgenome) path(consolidated_bcf_index) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 0fa9cd1..acf11ca 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -175,8 +175,8 @@ workflow SNVPHYL { // .map{} iterates onces, because there is only one item (one list) in the channel // .collect{} transforms each item in the list with the operation // which ultimately unzips the tuples - consolidated_bcfs_metas = consolidated_bcfs.map{ it.collect { it[0] } } - consolidated_bcfs_paths = consolidated_bcfs.map{ it.collect { it[1] } } + consolidated_bcfs_metas = consolidated_bcfs.collect{ it.collect { it[0] } } + consolidated_bcfs_paths = consolidated_bcfs.collect{ it.collect { it[1] } } //13. consolidate variant calling files VCF2SNV_ALIGNMENT( From a41ea58197dd626a5cd1e612345d63a370be1e4e Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 24 Jan 2024 10:35:04 -0600 Subject: [PATCH 067/113] Updating comment --- workflows/snvphylnfc.nf | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index acf11ca..63bf758 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -172,9 +172,8 @@ workflow SNVPHYL { consolidated_bcfs = CONSOLIDATE_BCFS.out.consolidated_bcfs.toSortedList{a, b -> a[0].id <=> b[0].id} // consolidated_bcfs is a list of [meta, filepath] tuples - // .map{} iterates onces, because there is only one item (one list) in the channel - // .collect{} transforms each item in the list with the operation - // which ultimately unzips the tuples + // the first .collect{} iterates once, because there is only one item (one list) in the channel + // the second .collect{} transforms each item in the list with the operation consolidated_bcfs_metas = consolidated_bcfs.collect{ it.collect { it[0] } } consolidated_bcfs_paths = consolidated_bcfs.collect{ it.collect { it[1] } } From d00eeb2682295b7f1473d2467d7196a4a7bc60bf Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 25 Jan 2024 13:35:27 -0600 Subject: [PATCH 068/113] First pass of adding reference selection by sample's associated assembly. --- assets/schema_input.json | 13 ++++++++++ nextflow.config | 3 ++- nextflow_schema.json | 6 +++++ workflows/snvphylnfc.nf | 54 ++++++++++++++++++++++++++++++++-------- 4 files changed, 65 insertions(+), 11 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 49897d8..6e7f68d 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -31,6 +31,19 @@ "maxLength": 0 } ] + }, + "assembly": { + "errorMessage": "Assembly file associated with this sample and must have the extension: '.fa', '.fasta', '.fa.gz' or '.fasta.gz'", + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.f(ast)?a(\\.gz)?$" + }, + { + "type": "string", + "maxLength": 0 + } + ] } }, "required": ["sample", "fastq_1"] diff --git a/nextflow.config b/nextflow.config index de2e4bf..45538de 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,7 +11,8 @@ params { // Input options input = null - refgenome = "${baseDir}/assets/reference.fasta" + refgenome = null + reference_sample_name = null // Pipeline parameters window_size = 11 diff --git a/nextflow_schema.json b/nextflow_schema.json index 6bed7bc..e78b4ca 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -36,6 +36,12 @@ "pattern": "^\\S+\\.(fa|fas|fasta)$", "format": "file-path" }, + "reference_sample_name": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "The sample ID from which to use the associated FASTA-format assembly as a reference.", + "pattern": "^\\S+$" + }, "email": { "type": "string", "description": "Email address for completion summary.", diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 63bf758..284ea22 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -87,23 +87,33 @@ workflow SNVPHYL { // NB: `input` corresponds to `params.input` and associated sample sheet schema input = Channel.fromSamplesheet("input") // Map the inputs so that they conform to the nf-core-expected "reads" format. - // Either [meta, [fastq_1]] or [meta, [fastq_1, fastq_2]] if fastq_2 exists - .map { meta, fastq_1, fastq_2 -> - fastq_2 ? tuple(meta, [ file(fastq_1), file(fastq_2) ]) : - tuple(meta, [ file(fastq_1) ])} + // Either [meta, [fastq_1], assembly] + // or [meta, [fastq_1, fastq_2], assembly] if fastq_2 exists + .map { meta, fastq_1, fastq_2, assembly -> + fastq_2 ? tuple(meta, [ file(fastq_1), file(fastq_2) ], assembly) : + tuple(meta, [ file(fastq_1) ], assembly)} + + // A channel of read tuples (meta, [fastq_1, fastq_2*]): + reads = input.map { meta, reads, assembly -> tuple(meta, reads) } + + // A [meta.id: assembly (URI path)] map: + sample_assemblies = input.toList().collectEntries { meta, reads, assembly -> [meta.id, assembly] } + + // Select the reference genome: + reference_genome = select_reference(params.refgenome, params.reference_sample_name, sample_assemblies) INDEXING( - params.refgenome + reference_genome ) ch_versions = ch_versions.mix(INDEXING.out.versions) FIND_REPEATS( - params.refgenome + reference_genome ) ch_versions = ch_versions.mix(FIND_REPEATS.out.versions) SMALT_MAP( - input, INDEXING.out.ref_fai, INDEXING.out.ref_sma, INDEXING.out.ref_smi + reads, INDEXING.out.ref_fai, INDEXING.out.ref_sma, INDEXING.out.ref_smi ) ch_versions = ch_versions.mix(SMALT_MAP.out.versions) @@ -118,7 +128,7 @@ workflow SNVPHYL { ch_versions = ch_versions.mix(VERIFYING_MAP_Q.out.versions) FREEBAYES( - SORT_INDEX_BAMS.out.sorted_bams_and_sampleID, params.refgenome + SORT_INDEX_BAMS.out.sorted_bams_and_sampleID, reference_genome ) ch_versions = ch_versions.mix(FREEBAYES.out.versions) @@ -139,7 +149,7 @@ workflow SNVPHYL { ch_versions = ch_versions.mix(FREEBAYES_VCF_TO_BCF.out.versions) MPILEUP( - SORT_INDEX_BAMS.out.sorted_bams_and_sampleID, params.refgenome + SORT_INDEX_BAMS.out.sorted_bams_and_sampleID, reference_genome ) ch_versions = ch_versions.mix(MPILEUP.out.versions) @@ -179,7 +189,7 @@ workflow SNVPHYL { //13. consolidate variant calling files VCF2SNV_ALIGNMENT( - consolidated_bcfs_metas, consolidated_bcfs_paths, invalid_positions_file, params.refgenome, + consolidated_bcfs_metas, consolidated_bcfs_paths, invalid_positions_file, reference_genome, CONSOLIDATE_BCFS.out.consolidated_bcf_index.collect() ) ch_versions = ch_versions.mix(VCF2SNV_ALIGNMENT.out.versions) @@ -208,6 +218,30 @@ workflow SNVPHYL { ) } +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + SELECT REFERENCE +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +def select_reference(refgenome, reference_sample_name, sample_assemblies) { + reference_genome = null + + // A "--refgenome" parameter was provided: + // Use the passed parameter. + if( refgenome != null ) { + reference_genome = refgenome + } + // A "--reference_sample_name" parameter was provided and the key exists in the assemblies map: + // Use the value (the assembly path) associated with the key (the sample name). + else if (reference_sample_name != null && + sample_assemblies.containsKey(reference_sample_name)) { + reference_genome = sample_assemblies[reference_sample_name] + } + + return reference_genome +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ COMPLETION EMAIL AND SUMMARY From f4a92e81438038c6c59cee91c8bfbd2ab691881e Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 10:24:44 -0600 Subject: [PATCH 069/113] Select reference by module solution. --- assets/schema_input.json | 3 ++ modules/local/selectreference/main.nf | 46 +++++++++++++++++++++++++++ workflows/snvphylnfc.nf | 36 ++++++--------------- 3 files changed, 58 insertions(+), 27 deletions(-) create mode 100644 modules/local/selectreference/main.nf diff --git a/assets/schema_input.json b/assets/schema_input.json index 6e7f68d..a7f1bca 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -17,6 +17,7 @@ "fastq_1": { "type": "string", "pattern": "^\\S+\\.f(ast)?q(\\.gz)?$", + "format": "file-path", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have the extension: '.fq', '.fastq', '.fq.gz' or '.fastq.gz'" }, "fastq_2": { @@ -24,6 +25,7 @@ "anyOf": [ { "type": "string", + "format": "file-path", "pattern": "^\\S+\\.f(ast)?q(\\.gz)?$" }, { @@ -37,6 +39,7 @@ "anyOf": [ { "type": "string", + "format": "file-path", "pattern": "^\\S+\\.f(ast)?a(\\.gz)?$" }, { diff --git a/modules/local/selectreference/main.nf b/modules/local/selectreference/main.nf new file mode 100644 index 0000000..f7a8f67 --- /dev/null +++ b/modules/local/selectreference/main.nf @@ -0,0 +1,46 @@ +process SELECT_REFERENCE { + label 'process_single' + + input: + val(parameters) + val(sample_assemblies) + + output: + val(reference_genome) + + exec: + refgenome = parameters.refgenome + reference_sample_name = parameters.reference_sample_name + + // A "--refgenome" parameter was provided: + if( refgenome != null ) { + reference_genome = refgenome + log.info("The '--reference_genome' parameter was provided and ${reference_genome} was selected as the reference.") + } + // A "--reference_sample_name" parameter was provided: + else if (reference_sample_name != null) { + + // Check to see if the sample ID exists: + if (sample_assemblies.containsKey(reference_sample_name)) { + + // Check if the sample ID maps to an assembly path: + // Use the value (the assembly path) associated with the key (the sample name). + if (sample_assemblies[reference_sample_name] != null) { + reference_genome = sample_assemblies[reference_sample_name] + log.info("The '--reference_sample_name' parameter was provided and ${reference_genome} was selected as the reference.") + } + // The sample ID exists, but has no corresponding assembly path: + else { + log.error("Unable to select a reference. The sample ID (${reference_sample_name}) has no associated assembly to use as a reference.") + } + } + // The sample ID does not exist: + else { + log.error("Unable to select a reference. The sample ID (${reference_sample_name}) does not exist.") + } + } + // Neither a "--refgenome" nor a "--reference_sample_name" were provided: + else { + log.error("Unable to select a reference. Neither a '--refgenome' nor a '--reference_sample_name' parameter were provided.") + } +} \ No newline at end of file diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 284ea22..6d61824 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -43,6 +43,7 @@ WorkflowSnvphylnfc.initialise(params, log) // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { SELECT_REFERENCE } from '../modules/local/selectreference/main' include { INDEXING } from '../modules/local/indexing/main' include { FIND_REPEATS } from '../modules/local/findrepeats/main' include { SMALT_MAP } from '../modules/local/smaltmap/main' @@ -96,11 +97,15 @@ workflow SNVPHYL { // A channel of read tuples (meta, [fastq_1, fastq_2*]): reads = input.map { meta, reads, assembly -> tuple(meta, reads) } - // A [meta.id: assembly (URI path)] map: - sample_assemblies = input.toList().collectEntries { meta, reads, assembly -> [meta.id, assembly] } + // A channel of samples mapped to their associated assemblies (if they exist): + sample_assemblies = input.toList().collectEntries { meta, reads, assembly -> [meta.id, assembly ? assembly : null] } + sample_assemblies.view() - // Select the reference genome: - reference_genome = select_reference(params.refgenome, params.reference_sample_name, sample_assemblies) + reference_genome = SELECT_REFERENCE( + params, sample_assemblies + ) + + reference_genome.ifEmpty { error("Unable to select a reference!") } INDEXING( reference_genome @@ -216,30 +221,7 @@ workflow SNVPHYL { CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - SELECT REFERENCE -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -def select_reference(refgenome, reference_sample_name, sample_assemblies) { - reference_genome = null - - // A "--refgenome" parameter was provided: - // Use the passed parameter. - if( refgenome != null ) { - reference_genome = refgenome - } - // A "--reference_sample_name" parameter was provided and the key exists in the assemblies map: - // Use the value (the assembly path) associated with the key (the sample name). - else if (reference_sample_name != null && - sample_assemblies.containsKey(reference_sample_name)) { - reference_genome = sample_assemblies[reference_sample_name] - } - return reference_genome } /* From 671cbaa8acc82ac9021d2e665c77ae48e0235c3c Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 13:16:44 -0600 Subject: [PATCH 070/113] Adding channel-based sample reference selection. --- workflows/snvphylnfc.nf | 39 +++++++++++++++++++++++++++++---------- 1 file changed, 29 insertions(+), 10 deletions(-) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 6d61824..1bbb84a 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -94,18 +94,13 @@ workflow SNVPHYL { fastq_2 ? tuple(meta, [ file(fastq_1), file(fastq_2) ], assembly) : tuple(meta, [ file(fastq_1) ], assembly)} - // A channel of read tuples (meta, [fastq_1, fastq_2*]): - reads = input.map { meta, reads, assembly -> tuple(meta, reads) } + // Channel of read tuples (meta, [fastq_1, fastq_2*]): + reads = input.map { meta, reads, assembly -> tuple(meta, reads) } - // A channel of samples mapped to their associated assemblies (if they exist): - sample_assemblies = input.toList().collectEntries { meta, reads, assembly -> [meta.id, assembly ? assembly : null] } - sample_assemblies.view() + // Channel of sample tuples (sample ID, assembly): + sample_assemblies = input.map { meta, reads, assembly -> tuple(meta.id, assembly ? assembly : null) } - reference_genome = SELECT_REFERENCE( - params, sample_assemblies - ) - - reference_genome.ifEmpty { error("Unable to select a reference!") } + reference_genome = select_reference(params.refgenome, params.reference_sample_name, sample_assemblies) INDEXING( reference_genome @@ -224,6 +219,30 @@ workflow SNVPHYL { } +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + SELECT REFERENCE +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +def select_reference(refgenome, reference_sample_name, sample_assemblies) { + + if(refgenome) { + reference_genome = Channel.value(refgenome) + } + else if (reference_sample_name) { + reference_genome = sample_assemblies.filter { it[0] == reference_sample_name && it[1] != null} + .ifEmpty { error("The provided reference sample ID (${reference_sample_name}) is either missing or has no associated assembly.") } + .map { it[1] } + .first() + } + else { + error("Unable to select a reference. Neither '--refgenome' nor '--reference_sample_name' were provided.") + } + + return reference_genome +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ COMPLETION EMAIL AND SUMMARY From b2b527734d8e8ded000c40ca96a47266b138c592 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 13:17:45 -0600 Subject: [PATCH 071/113] Removing old module solution. --- modules/local/selectreference/main.nf | 46 --------------------------- workflows/snvphylnfc.nf | 1 - 2 files changed, 47 deletions(-) delete mode 100644 modules/local/selectreference/main.nf diff --git a/modules/local/selectreference/main.nf b/modules/local/selectreference/main.nf deleted file mode 100644 index f7a8f67..0000000 --- a/modules/local/selectreference/main.nf +++ /dev/null @@ -1,46 +0,0 @@ -process SELECT_REFERENCE { - label 'process_single' - - input: - val(parameters) - val(sample_assemblies) - - output: - val(reference_genome) - - exec: - refgenome = parameters.refgenome - reference_sample_name = parameters.reference_sample_name - - // A "--refgenome" parameter was provided: - if( refgenome != null ) { - reference_genome = refgenome - log.info("The '--reference_genome' parameter was provided and ${reference_genome} was selected as the reference.") - } - // A "--reference_sample_name" parameter was provided: - else if (reference_sample_name != null) { - - // Check to see if the sample ID exists: - if (sample_assemblies.containsKey(reference_sample_name)) { - - // Check if the sample ID maps to an assembly path: - // Use the value (the assembly path) associated with the key (the sample name). - if (sample_assemblies[reference_sample_name] != null) { - reference_genome = sample_assemblies[reference_sample_name] - log.info("The '--reference_sample_name' parameter was provided and ${reference_genome} was selected as the reference.") - } - // The sample ID exists, but has no corresponding assembly path: - else { - log.error("Unable to select a reference. The sample ID (${reference_sample_name}) has no associated assembly to use as a reference.") - } - } - // The sample ID does not exist: - else { - log.error("Unable to select a reference. The sample ID (${reference_sample_name}) does not exist.") - } - } - // Neither a "--refgenome" nor a "--reference_sample_name" were provided: - else { - log.error("Unable to select a reference. Neither a '--refgenome' nor a '--reference_sample_name' parameter were provided.") - } -} \ No newline at end of file diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 1bbb84a..4adada9 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -43,7 +43,6 @@ WorkflowSnvphylnfc.initialise(params, log) // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // include { INPUT_CHECK } from '../subworkflows/local/input_check' -include { SELECT_REFERENCE } from '../modules/local/selectreference/main' include { INDEXING } from '../modules/local/indexing/main' include { FIND_REPEATS } from '../modules/local/findrepeats/main' include { SMALT_MAP } from '../modules/local/smaltmap/main' From 6fc184401891fbc7262b7090ae2bb55195391a1b Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 13:22:01 -0600 Subject: [PATCH 072/113] Changing reference_sample_name to reference_sample_id --- nextflow.config | 2 +- nextflow_schema.json | 2 +- workflows/snvphylnfc.nf | 12 ++++++------ 3 files changed, 8 insertions(+), 8 deletions(-) diff --git a/nextflow.config b/nextflow.config index 45538de..f68520a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,7 +12,7 @@ params { // Input options input = null refgenome = null - reference_sample_name = null + reference_sample_id = null // Pipeline parameters window_size = 11 diff --git a/nextflow_schema.json b/nextflow_schema.json index e78b4ca..9ff55af 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -36,7 +36,7 @@ "pattern": "^\\S+\\.(fa|fas|fasta)$", "format": "file-path" }, - "reference_sample_name": { + "reference_sample_id": { "type": "string", "fa_icon": "fas fa-file", "description": "The sample ID from which to use the associated FASTA-format assembly as a reference.", diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 4adada9..9bb8d18 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -99,7 +99,7 @@ workflow SNVPHYL { // Channel of sample tuples (sample ID, assembly): sample_assemblies = input.map { meta, reads, assembly -> tuple(meta.id, assembly ? assembly : null) } - reference_genome = select_reference(params.refgenome, params.reference_sample_name, sample_assemblies) + reference_genome = select_reference(params.refgenome, params.reference_sample_id, sample_assemblies) INDEXING( reference_genome @@ -224,19 +224,19 @@ workflow SNVPHYL { ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -def select_reference(refgenome, reference_sample_name, sample_assemblies) { +def select_reference(refgenome, reference_sample_id, sample_assemblies) { if(refgenome) { reference_genome = Channel.value(refgenome) } - else if (reference_sample_name) { - reference_genome = sample_assemblies.filter { it[0] == reference_sample_name && it[1] != null} - .ifEmpty { error("The provided reference sample ID (${reference_sample_name}) is either missing or has no associated assembly.") } + else if (reference_sample_id) { + reference_genome = sample_assemblies.filter { it[0] == reference_sample_id && it[1] != null} + .ifEmpty { error("The provided reference sample ID (${reference_sample_id}) is either missing or has no associated assembly.") } .map { it[1] } .first() } else { - error("Unable to select a reference. Neither '--refgenome' nor '--reference_sample_name' were provided.") + error("Unable to select a reference. Neither '--refgenome' nor '--reference_sample_id' were provided.") } return reference_genome From 3cee198e9891f8e388fddf1f5d878c0cad4f1a29 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 13:40:05 -0600 Subject: [PATCH 073/113] Fixes one Path in List being iterated as String. --- modules/local/vcf2snvalignment/main.nf | 5 +---- modules/local/verifyingmapq/main.nf | 5 +---- 2 files changed, 2 insertions(+), 8 deletions(-) diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index f4ccad6..f86ff6a 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -28,10 +28,7 @@ process VCF2SNV_ALIGNMENT { script: def bcf_line = "" - - for (int i = 0; i < bcf_paths.size(); i++) { - bcf_line += "--consolidate_vcf ${bcf_metas[i].id}=${bcf_paths[i]} " - } + bcf_paths.eachWithIndex { path, i -> bcf_line += "--consolidate_vcf ${bcf_metas[i].id}=${path} " } """ vcf2snv_alignment.pl --reference reference --invalid-pos ${new_invalid_positions} --format fasta --format phylip --numcpus ${task.cpus} --output-base snvalign --fasta ${refgenome} ${bcf_line} diff --git a/modules/local/verifyingmapq/main.nf b/modules/local/verifyingmapq/main.nf index ac843aa..05f1700 100644 --- a/modules/local/verifyingmapq/main.nf +++ b/modules/local/verifyingmapq/main.nf @@ -21,10 +21,7 @@ process VERIFYING_MAP_Q { script: def bam_line = "" - - for (int i = 0; i < sorted_bams.size(); i++) { - bam_line += "--bam ${sorted_bams[i].getName()}=${sorted_bams[i]} " - } + sorted_bams.each { bam_line += "--bam ${it.getName()}=${it} " } """ verify_mapping_quality.pl -c ${task.cpus} --min-depth ${params.min_coverage_depth} --min-map ${params.min_mapping_percent_cov} --output mappingQuality.txt ${bam_line} From 409864a836dd4c2cd6191e7456881b973ca04748 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 13:45:41 -0600 Subject: [PATCH 074/113] Updating samplesheet for testing. --- assets/samplesheet.csv | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index fd365a5..7708c95 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,4 +1,4 @@ -sample,fastq_1,fastq_2 -SAMPLE1,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_2.fastq -SAMPLE2,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_2.fastq -SAMPLE3,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_2.fastq +sample,fastq_1,fastq_2,assembly +SAMPLE1,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_2.fastq,https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta +SAMPLE2,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_2.fastq, +SAMPLE3,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_2.fastq, From 30d80f4be72d9742a42478c5a065706ba912cef1 Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 13:54:53 -0600 Subject: [PATCH 075/113] Adding integration test for selecting with sample ID. --- tests/workflows/snvphylnfc.nf.test | 64 +++++++++++++++++++++++++++++- 1 file changed, 63 insertions(+), 1 deletion(-) diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index 9d22093..b15f8f7 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -4,7 +4,7 @@ nextflow_workflow { script "workflows/snvphylnfc.nf" workflow "SNVPHYL" - test("basic integration test") { + test("selecting reference by refgenome") { when { params { @@ -66,4 +66,66 @@ nextflow_workflow { } + test("selecting reference by sample id") { + + when { + params { + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/409864a836dd4c2cd6191e7456881b973ca04748/assets/samplesheet.csv" + reference_sample_id = "SAMPLE1" + outdir = "results" + } + workflow {} + } + + then { + assert workflow.success + assert path("$launchDir/results").exists() + + def lines = [] + + // check FILTER_STATS output + lines = path("$launchDir/results/filter/filterStats.txt").readLines() + + assert lines.contains("Number of sites used to generate phylogeny: 2") + assert lines.contains("Total number of sites identified: 2") + assert lines.contains("Number of sites filtered: 0") + + // check PHYML output + lines = path("$launchDir/results/phyml/phylogeneticTreeStats.txt").readLines() + + assert lines.contains(". Model of nucleotides substitution: \tGTR") + assert lines.contains(" - f(A)= 0.75000") + assert lines.contains(" - f(C)= 0.12500") + assert lines.contains(" - f(G)= 0.01000") + assert lines.contains(" - f(T)= 0.12500") + + lines = path("$launchDir/results/phyml/phylogeneticTree.newick").readLines() + + assert lines.join("\n").contains("SAMPLE1") + assert lines.join("\n").contains("SAMPLE2") + assert lines.join("\n").contains("SAMPLE3") + + // check MAKE_SNV output + lines = path("$launchDir/results/make/snvMatrix.tsv").readLines() + + assert lines[0] == "strain\tSAMPLE2\tSAMPLE3\tSAMPLE1\treference\t" + assert lines.contains("SAMPLE1\t1\t1\t0\t0\t") + assert lines.contains("SAMPLE2\t0\t2\t1\t1\t") + assert lines.contains("SAMPLE3\t2\t0\t1\t1\t") + + // check IRIDA Next JSON file + lines = path("$launchDir/results/iridanext.output.json.gz").linesGzip.join("\n") + + assert lines.contains("\"path\": \"make/snvMatrix.tsv\"") + assert lines.contains("\"path\": \"filter/filterStats.txt\"") + assert lines.contains("\"path\": \"phyml/phylogeneticTreeStats.txt\"") + assert lines.contains("\"path\": \"phyml/phylogeneticTree.newick\"") + assert lines.contains("\"path\": \"vcf2snv/snvTable.tsv\"") + assert lines.contains("\"path\": \"vcf2snv/vcf2core.tsv\"") + assert lines.contains("\"path\": \"vcf2snv/snvAlignment.phy\"") + assert lines.contains("\"path\": \"verifying/mappingQuality.txt\"") + } + + } + } From 80924e7675ec79a12d86cb05d102c40dcc8e44cf Mon Sep 17 00:00:00 2001 From: Eric Date: Fri, 26 Jan 2024 14:00:55 -0600 Subject: [PATCH 076/113] Updating test profile test. --- conf/test.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/test.config b/conf/test.config index 63441f3..350ffc7 100644 --- a/conf/test.config +++ b/conf/test.config @@ -21,4 +21,5 @@ params { // Input data input = 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/samplesheet.csv' + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" } From fe78bc0969dd0efcc91ffa913754c225c8a633dc Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 30 Jan 2024 09:29:34 -0600 Subject: [PATCH 077/113] Grouping the reference parameters together. --- nextflow_schema.json | 28 ++++++++++++++++++++-------- 1 file changed, 20 insertions(+), 8 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 9ff55af..a9a5b68 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -29,6 +29,21 @@ "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", "fa_icon": "fas fa-folder-open" }, + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + } + } + }, + "new_group_1": { + "title": "New Group 1", + "type": "object", + "description": "The reference to use for analysis. Use only one method for selecting the reference.", + "default": "", + "properties": { "refgenome": { "type": "string", "description": "The file path to the FASTA-format reference genome to use.", @@ -41,15 +56,9 @@ "fa_icon": "fas fa-file", "description": "The sample ID from which to use the associated FASTA-format assembly as a reference.", "pattern": "^\\S+$" - }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" } - } + }, + "fa_icon": "fas fa-terminal" }, "snvphyl_pipeline_parameters": { "title": "SNVPhyl pipeline parameters", @@ -277,6 +286,9 @@ { "$ref": "#/definitions/input_output_options" }, + { + "$ref": "#/definitions/new_group_1" + }, { "$ref": "#/definitions/snvphyl_pipeline_parameters" }, From 708c420ebf8edce68f4e290a2c4e5310989540fa Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 30 Jan 2024 09:58:07 -0600 Subject: [PATCH 078/113] Adding test for when no reference is selected. --- tests/workflows/snvphylnfc.nf.test | 17 +++++++++++++++++ 1 file changed, 17 insertions(+) diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index b15f8f7..0dd4c55 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -128,4 +128,21 @@ nextflow_workflow { } + test("selecting no reference (failure)") { + + when { + params { + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/409864a836dd4c2cd6191e7456881b973ca04748/assets/samplesheet.csv" + outdir = "results" + } + workflow {} + } + + then { + assert workflow.failed + assert workflow.stdout.contains("Unable to select a reference. Neither '--refgenome' nor '--reference_sample_id' were provided.") + } + + } + } From 1da07b49d29bf659c2091e6ffe2dce4028688962 Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 30 Jan 2024 10:28:26 -0600 Subject: [PATCH 079/113] Added file wraps and debug statements related to reference selection. --- workflows/snvphylnfc.nf | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 9bb8d18..04acf94 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -91,7 +91,7 @@ workflow SNVPHYL { // or [meta, [fastq_1, fastq_2], assembly] if fastq_2 exists .map { meta, fastq_1, fastq_2, assembly -> fastq_2 ? tuple(meta, [ file(fastq_1), file(fastq_2) ], assembly) : - tuple(meta, [ file(fastq_1) ], assembly)} + tuple(meta, [ file(fastq_1) ], file(assembly))} // Channel of read tuples (meta, [fastq_1, fastq_2*]): reads = input.map { meta, reads, assembly -> tuple(meta, reads) } @@ -227,13 +227,15 @@ workflow SNVPHYL { def select_reference(refgenome, reference_sample_id, sample_assemblies) { if(refgenome) { - reference_genome = Channel.value(refgenome) + reference_genome = Channel.value(file(refgenome)) + log.debug "Selecting reference genome ${reference_genome} from '--refgenome'." } else if (reference_sample_id) { reference_genome = sample_assemblies.filter { it[0] == reference_sample_id && it[1] != null} .ifEmpty { error("The provided reference sample ID (${reference_sample_id}) is either missing or has no associated assembly.") } .map { it[1] } .first() + log.debug "Selecting reference genome ${reference_genome} from '--reference_sample_id'." } else { error("Unable to select a reference. Neither '--refgenome' nor '--reference_sample_id' were provided.") From 8d15dccbf7665d39e5073ba93734753502665d89 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 1 Feb 2024 12:15:36 -0600 Subject: [PATCH 080/113] Group title --- nextflow_schema.json | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index a9a5b68..b576296 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -38,8 +38,8 @@ } } }, - "new_group_1": { - "title": "New Group 1", + "reference": { + "title": "Reference", "type": "object", "description": "The reference to use for analysis. Use only one method for selecting the reference.", "default": "", @@ -287,7 +287,7 @@ "$ref": "#/definitions/input_output_options" }, { - "$ref": "#/definitions/new_group_1" + "$ref": "#/definitions/reference" }, { "$ref": "#/definitions/snvphyl_pipeline_parameters" From acfa8622675ed3a3eb8bb9f80e604d01b3e04e6e Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 1 Feb 2024 12:57:31 -0600 Subject: [PATCH 081/113] Fixing linting. --- .github/workflows/linting.yml | 65 ++++++-------------------- .github/workflows/linting_comment.yml | 2 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes nextflow_schema.json | 6 +-- pyproject.toml | 17 ++++--- 5 files changed, 27 insertions(+), 63 deletions(-) delete mode 100644 lib/nfcore_external_java_deps.jar diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 00fdd61..81cd098 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,72 +11,33 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude LICENSE-Apachev2 README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile|LICENSE|tests\data\') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - uses: actions/setup-node@v3 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v3 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30..147bcd1 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest 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upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" @@ -179,7 +179,7 @@ "max_memory": { "type": "string", "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", + "default": "2.GB", "fa_icon": "fas fa-memory", "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", "hidden": true, @@ -188,7 +188,7 @@ "max_time": { "type": "string", "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", + "default": "1.h", "fa_icon": "far fa-clock", "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", "hidden": true, diff --git a/pyproject.toml b/pyproject.toml index 0d62beb..7d08e1c 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] From de507ed0b1e5d11860604a3e7e9e0b396a1391a7 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 5 Feb 2024 14:09:43 -0600 Subject: [PATCH 082/113] Updating schema descriptions to be more helpful and accurate. --- nextflow_schema.json | 66 ++++++++++++++++++++++---------------------- 1 file changed, 33 insertions(+), 33 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index b619064..f7fbb41 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -2,14 +2,14 @@ "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/nextflow_schema.json", "title": "phac-nml/snvphylnfc pipeline parameters", - "description": "IRIDA Next Example Pipeline", + "description": "SNVPhyl: Whole Genome SNV Phylogenomics Pipeline", "type": "object", "definitions": { "input_output_options": { - "title": "Input/output options", + "title": "Input/Output Options", "type": "object", "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", + "description": "Define which data to use with the pipeline.", "required": ["input", "outdir"], "properties": { "input": { @@ -44,69 +44,69 @@ "description": "The reference to use for analysis. Use only one method for selecting the reference.", "default": "", "properties": { - "refgenome": { - "type": "string", - "description": "The file path to the FASTA-format reference genome to use.", - "fa_icon": "fas fa-file", - "pattern": "^\\S+\\.(fa|fas|fasta)$", - "format": "file-path" - }, "reference_sample_id": { "type": "string", "fa_icon": "fas fa-file", "description": "The sample ID from which to use the associated FASTA-format assembly as a reference.", "pattern": "^\\S+$" + }, + "refgenome": { + "type": "string", + "description": "The file path to the FASTA-format reference genome.", + "fa_icon": "fas fa-file", + "pattern": "^\\S+\\.(fa|fas|fasta)$", + "format": "file-path" } }, "fa_icon": "fas fa-terminal" }, "snvphyl_pipeline_parameters": { - "title": "SNVPhyl pipeline parameters", + "title": "SNVPhyl Pipeline Parameters", "type": "object", - "description": "Parameters to configure specific pipeline behaviour.", + "description": "Fine-tuning of SNVPhyl's parameters to configure specific pipeline behaviour.", "default": "", "properties": { - "window_size": { + "min_coverage_depth": { "type": "integer", - "default": 11, - "description": "Used during BCF consolidation.", + "default": 5, "fa_icon": "fas fa-desktop", + "description": "The minimum depth of coverage for a position within the genome to pass the mapping quality check and be included in the analysis.", "minimum": 1 }, - "density_threshold": { + "min_mapping_percent_cov": { "type": "integer", - "default": 2, - "description": "Used during BCF consolidation.", - "fa_icon": "fas fa-desktop", - "minimum": 1 + "default": 80, + "description": "The total percentage of positions within the genome that must have a depth of coverage greater than the minimum depth of coverage specified in order to pass the mapping quality check and be included in the analysis.", + "minimum": 0, + "maximum": 100, + "fa_icon": "fas fa-desktop" }, - "min_coverage_depth": { + "min_mean_mapping_quality": { "type": "integer", - "default": 5, + "default": 30, "fa_icon": "fas fa-desktop", - "description": "The minimum depth of coverage for a VCF to be accepted.", + "description": "The minimum mean mapping quality score for all reads in a pileup to be included in the analysis.", "minimum": 1 }, - "min_mean_mapping_quality": { + "window_size": { "type": "integer", - "default": 30, + "default": 11, + "description": "The window size for determining whether a region is high density.", "fa_icon": "fas fa-desktop", - "description": "The minimum mean mapping quality.", "minimum": 1 }, - "min_mapping_percent_cov": { + "density_threshold": { "type": "integer", - "default": 80, - "description": "The minimum mapping percent coverage.", - "minimum": 0, - "maximum": 100, - "fa_icon": "fas fa-desktop" + "default": 2, + "description": "The minimum number of SNVs within the window size for a region to be considered high density.", + "fa_icon": "fas fa-desktop", + "minimum": 1 }, "snv_abundance_ratio": { "type": "number", "default": 0.75, "fa_icon": "fas fa-desktop", - "description": "The SNV abundance ratio.", + "description": "The proportion of reads required to support a variant to be included in the analysis.", "minimum": 0, "maximum": 1 }, From 79ab10770fa7bc7f700cddc55ff8c42ad878a790 Mon Sep 17 00:00:00 2001 From: Eric Date: Tue, 6 Feb 2024 14:17:58 -0600 Subject: [PATCH 083/113] SNV density filtering. --- conf/modules.config | 4 ++++ modules/local/consolidatebcfs/main.nf | 6 ++++-- nextflow.config | 1 + nextflow_schema.json | 5 +++++ workflows/snvphylnfc.nf | 2 +- 5 files changed, 15 insertions(+), 3 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index c4cb547..8989ef2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -29,4 +29,8 @@ process { pattern: '*_versions.yml' ] } + + withName: CONSOLIDATE_BCFS { + ext.args = { params.skip_density_filter ? "--skip-density-filter" : null } + } } diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index 1d64f18..6aeff9d 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -19,13 +19,15 @@ process CONSOLIDATE_BCFS { tuple val(meta), path( "${meta.id}_consolidated.bcf" ), emit: consolidated_bcfs path( "${meta.id}_consolidated.vcf" ) , emit: consolidated_vcfs path( "${meta.id}_consolidated.bcf.csi" ) , emit: consolidated_bcf_index - path( "${meta.id}_filtered_density.txt" ) , emit: filtered_densities + path( "${meta.id}_filtered_density.txt" ) , emit: filtered_densities, optional: true path("versions.yml") , emit: versions script: + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + """ - consolidate_vcfs.pl --coverage-cutoff ${params.min_coverage_depth} --min-mean-mapping ${params.min_mean_mapping_quality} --snv-abundance-ratio ${params.snv_abundance_ratio} --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf > ${prefix}_consolidated.vcf + consolidate_vcfs.pl --coverage-cutoff ${params.min_coverage_depth} --min-mean-mapping ${params.min_mean_mapping_quality} --snv-abundance-ratio ${params.snv_abundance_ratio} --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf ${args} > ${prefix}_consolidated.vcf bcftools index -f ${prefix}_consolidated.bcf cat <<-END_VERSIONS > versions.yml diff --git a/nextflow.config b/nextflow.config index f68520a..978e9a4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -23,6 +23,7 @@ params { snv_abundance_ratio = 0.75 min_repeat_length = 150 min_repeat_pid = 90 + skip_density_filter = false // Boilerplate options outdir = null diff --git a/nextflow_schema.json b/nextflow_schema.json index f7fbb41..f768dc2 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -124,6 +124,11 @@ "description": "The minimum percent identity when identifying repeats.", "minimum": 0, "maximum": 100 + }, + "skip_density_filter": { + "type": "boolean", + "description": "Whether or not to skip filtering low SNV density regions.", + "fa_icon": "fas fa-desktop" } }, "fa_icon": "fas fa-terminal" diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 04acf94..be2b41d 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -173,7 +173,7 @@ workflow SNVPHYL { // Concat filtered densities to make new invalid_postions invalid_positions = CONSOLIDATE_BCFS.out.filtered_densities .concat(FIND_REPEATS.out.repeats_bed_file) - .collect().map{it -> [[id: 'invalid_positions'], it]} + .collect().map{it -> [[id: 'cat_invalid_positions'], it]} CAT_CAT(invalid_positions) From f44fc8dfb24415850fb480f9ef3b73ba3ab533ad Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 7 Feb 2024 12:34:34 -0600 Subject: [PATCH 084/113] Adding simple test for skipping SNV density filtering. --- modules/local/consolidatebcfs/main.nf | 3 +-- tests/workflows/snvphylnfc.nf.test | 32 +++++++++++++++++++++++---- 2 files changed, 29 insertions(+), 6 deletions(-) diff --git a/modules/local/consolidatebcfs/main.nf b/modules/local/consolidatebcfs/main.nf index 6aeff9d..d6e6d0c 100644 --- a/modules/local/consolidatebcfs/main.nf +++ b/modules/local/consolidatebcfs/main.nf @@ -17,7 +17,6 @@ process CONSOLIDATE_BCFS { output: tuple val(meta), path( "${meta.id}_consolidated.bcf" ), emit: consolidated_bcfs - path( "${meta.id}_consolidated.vcf" ) , emit: consolidated_vcfs path( "${meta.id}_consolidated.bcf.csi" ) , emit: consolidated_bcf_index path( "${meta.id}_filtered_density.txt" ) , emit: filtered_densities, optional: true path("versions.yml") , emit: versions @@ -27,7 +26,7 @@ process CONSOLIDATE_BCFS { def prefix = task.ext.prefix ?: "${meta.id}" """ - consolidate_vcfs.pl --coverage-cutoff ${params.min_coverage_depth} --min-mean-mapping ${params.min_mean_mapping_quality} --snv-abundance-ratio ${params.snv_abundance_ratio} --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf ${args} > ${prefix}_consolidated.vcf + consolidate_vcfs.pl --coverage-cutoff ${params.min_coverage_depth} --min-mean-mapping ${params.min_mean_mapping_quality} --snv-abundance-ratio ${params.snv_abundance_ratio} --vcfsplit ${freebayes_filtered_bcf} --mpileup ${mpileup_bcf} --filtered-density-out ${prefix}_filtered_density.txt --window-size ${params.window_size} --density-threshold ${params.density_threshold} -o ${prefix}_consolidated.bcf ${args} bcftools index -f ${prefix}_consolidated.bcf cat <<-END_VERSIONS > versions.yml diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index 0dd4c55..a741824 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -8,8 +8,8 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/samplesheet.csv" - refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" } workflow {} @@ -70,7 +70,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/409864a836dd4c2cd6191e7456881b973ca04748/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" reference_sample_id = "SAMPLE1" outdir = "results" } @@ -132,7 +132,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/409864a836dd4c2cd6191e7456881b973ca04748/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" outdir = "results" } workflow {} @@ -145,4 +145,28 @@ nextflow_workflow { } + test("disabling snv density filtering") { + + when { + params { + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" + outdir = "results" + skip_density_filter = true + } + workflow {} + } + + then { + assert workflow.success + assert path("$launchDir/results").exists() + + // check that no "_filtered_density.txt" files exist: + assert path("$launchDir/results/consolidate/SAMPLE1_filtered_density.txt").exists() == false + assert path("$launchDir/results/consolidate/SAMPLE2_filtered_density.txt").exists() == false + assert path("$launchDir/results/consolidate/SAMPLE3_filtered_density.txt").exists() == false + } + + } + } From 01fc2abe09698ea07c57cde2149565dd22f9bdf1 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 7 Feb 2024 14:25:27 -0600 Subject: [PATCH 085/113] Adding checks for filtered density outputs to other integration tests. --- tests/workflows/snvphylnfc.nf.test | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index a741824..43b2015 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -28,6 +28,11 @@ nextflow_workflow { assert lines.contains("Total number of sites identified: 2") assert lines.contains("Number of sites filtered: 0") + // check CONSOLIDATE_BCFS for filtered density files: + assert path("$launchDir/results/consolidate/SAMPLE1_filtered_density.txt").exists() + assert path("$launchDir/results/consolidate/SAMPLE2_filtered_density.txt").exists() + assert path("$launchDir/results/consolidate/SAMPLE3_filtered_density.txt").exists() + // check PHYML output lines = path("$launchDir/results/phyml/phylogeneticTreeStats.txt").readLines() @@ -90,6 +95,11 @@ nextflow_workflow { assert lines.contains("Total number of sites identified: 2") assert lines.contains("Number of sites filtered: 0") + // check CONSOLIDATE_BCFS for filtered density files: + assert path("$launchDir/results/consolidate/SAMPLE1_filtered_density.txt").exists() + assert path("$launchDir/results/consolidate/SAMPLE2_filtered_density.txt").exists() + assert path("$launchDir/results/consolidate/SAMPLE3_filtered_density.txt").exists() + // check PHYML output lines = path("$launchDir/results/phyml/phylogeneticTreeStats.txt").readLines() From 3891cce31a1881bf07c4f52b68cd35eb9b96489b Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 7 Feb 2024 14:58:14 -0600 Subject: [PATCH 086/113] Improving test thoroughness. --- tests/workflows/snvphylnfc.nf.test | 15 +++++++++++++-- 1 file changed, 13 insertions(+), 2 deletions(-) diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index 43b2015..4e65bac 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -28,11 +28,14 @@ nextflow_workflow { assert lines.contains("Total number of sites identified: 2") assert lines.contains("Number of sites filtered: 0") - // check CONSOLIDATE_BCFS for filtered density files: + // check filtered density files: assert path("$launchDir/results/consolidate/SAMPLE1_filtered_density.txt").exists() assert path("$launchDir/results/consolidate/SAMPLE2_filtered_density.txt").exists() assert path("$launchDir/results/consolidate/SAMPLE3_filtered_density.txt").exists() + lines = path("$launchDir/results/cat/cat_invalid_positions.txt").readLines() + assert lines.contains("#Calculation and writing of high density regions has completed.") + // check PHYML output lines = path("$launchDir/results/phyml/phylogeneticTreeStats.txt").readLines() @@ -95,11 +98,14 @@ nextflow_workflow { assert lines.contains("Total number of sites identified: 2") assert lines.contains("Number of sites filtered: 0") - // check CONSOLIDATE_BCFS for filtered density files: + // check filtered density files: assert path("$launchDir/results/consolidate/SAMPLE1_filtered_density.txt").exists() assert path("$launchDir/results/consolidate/SAMPLE2_filtered_density.txt").exists() assert path("$launchDir/results/consolidate/SAMPLE3_filtered_density.txt").exists() + lines = path("$launchDir/results/cat/cat_invalid_positions.txt").readLines() + assert lines.contains("#Calculation and writing of high density regions has completed.") + // check PHYML output lines = path("$launchDir/results/phyml/phylogeneticTreeStats.txt").readLines() @@ -168,6 +174,8 @@ nextflow_workflow { } then { + def lines = [] + assert workflow.success assert path("$launchDir/results").exists() @@ -175,6 +183,9 @@ nextflow_workflow { assert path("$launchDir/results/consolidate/SAMPLE1_filtered_density.txt").exists() == false assert path("$launchDir/results/consolidate/SAMPLE2_filtered_density.txt").exists() == false assert path("$launchDir/results/consolidate/SAMPLE3_filtered_density.txt").exists() == false + + lines = path("$launchDir/results/cat/cat_invalid_positions.bed").readLines() + assert lines.contains("#Calculation and writing of high density regions has completed.") == false } } From 28d34a6bb147f6a5be71ebace6f7465c3eb8eec5 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 11:58:22 -0600 Subject: [PATCH 087/113] Updating the README --- README.md | 130 +++++++++++++++++++++++++++++++++++++++++++++++++++++- 1 file changed, 129 insertions(+), 1 deletion(-) diff --git a/README.md b/README.md index ca2f74c..9b4c1a1 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,135 @@ # SNVPhyl nf-core Pipeline -This is the in-development nf-core-based pipeline for SNVPhyl. +This is the [nf-core](https://nf-co.re/)-based pipeline for [SNVPhyl](https://snvphyl.readthedocs.io/en/). The SNVPhyl (Single Nucleotide Variant PHYLogenomics) pipeline identifies Single Nucleotide Variants (SNV) within a collection of microbial genomes and constructs a phylogenetic tree from those SNVs. This pipeline is designed to be integrated into [IRIDA Next](https://github.com/phac-nml/irida-next). However, it may be run as a stand-alone pipeline. + +# Input + +Input is provided to SNVPhyl in the form of a sample sheet (passed as `--input samplesheet.csv`). This samplesheet is a CSV-formated file, which may be provided as a URI (ex: a file path or web address), and has the following format: + +| sample | fastq_1 | fastq_2 | assembly | +|---------|----------------------------|----------------------------|------------------------------| +| SAMPLE1 | /path/to/sample1_fastq1.fq | /path/to/sample1_fastq2.fq | /path/to/sample1_assembly.fa | +| SAMPLE2 | /path/to/sample2_fastq1.fq | | | + +The columns are defined as follows: + +- sample: The unique sample identifier to associate with the reads (and optionally the assembly). +- fastq_1: A URI (ex: a file path or web address) to either single-end FASTQ-formatted reads or one pair of pair-end FASTQ-formatted reads. +- fastq_2: (Optional) If `fastq_1` is paired-end, then this field is a URI to reads that are the other pair of reads associated with `fastq_1`. +- assembly: (Optional) A URI to an assembly associated with the sample, so that it may be referenced on the command line by the sample identifier for use as the reference for the whole pipeline. However, it may be easier to leave these fields blank and specify the reference using the `--refgenome` parameter. + +The structure of this file is defined in [assets/schema_input.json](assets/schema_input.json). Please see [assets/samplesheet.csv](assets/samplesheet.csv) to see an example of a samplesheet for this pipeline. + +# Parameters + +## Mandatory + +The mandatory parameters are as follows: + +- `--input`: a URI to the samplesheet +- `--output`: the directory for pipeline output + +## Optional + +The optional parameters are as follows: + +### Reference + +- `--refgenome`: a URI to the reference genome to use during pipeline analysis +- `--reference_sample_id`: the sample identifier of a sample in the samplesheet that contains a provided `assembly` to use as a reference genome during pipeline analysis + +Please use only one of `--refgenome` or `--reference_sample_id` and not both. + +### SNVPhyl Parameters + +- `--window_size`: The window size for determining whether a region is high density. +- `--density_threshold`: The minimum number of SNVs within the window size for a region to be considered high density. +- `--min_coverage_depth`: The minimum depth of coverage for a position within the genome to pass the mapping quality check and be included in the analysis. +- `--min_mapping_percent_cov`: The total percentage of positions within the genome that must have a depth of coverage greater than the minimum depth of coverage specified in order to pass the mapping quality check and be included in the analysis. +- `--min_mean_mapping_quality`: The minimum mean mapping quality score for all reads in a pileup to be included in the analysis. +- `--snv_abundance_ratio`: The proportion of reads required to support a variant to be included in the analysis. +- `--min_repeat_length`: The minimum length when identifying repeats. +- `--min_repeat_pid`: The minimum percent identity when identifying repeats. +- `--skip_density_filter`: Whether or not to skip filtering low SNV density regions. + +Please refer to the [SNVPhyl documentation](https://snvphyl.readthedocs.io/en/latest/) for more detailed information about pipeline parameters. + +# Running + +## Test Data + +In order to run the pipeline with provided data, please run: + +``` +nextflow run main.nf -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results +``` + +The pipeline output will be written to a directory named `results`. A JSON file for integrating data with IRIDA Next will be written to `results/iridanext.output.json.gz` (please see the (Output)[#Output] section for details). + +# Output + +## Results + +The following output files are generated by the pipeline: + +- `make/snvMatrix.tsv`: a pair-wise distance matrix of SNVs that passed all filtering criteria +- `filter/filterStats.txt`: a summary of the number of SNVs filtered within in the SNV Table +- `phyml/phylogeneticTree.newick`: the maximum likelihood phylogeny generated from an alignment of SNVs extracted from the whole genomes of each input file +- `phyml/phylogeneticTreeStats.txt`: statistics for the generated phylogenetic tree +- `vcf2snv/snvTable.tsv`: a table of all detected variant sites +- `vcf2snv/vcf2core.tsv`: a table of the evaluated core positions in each reference fasta sequence +- `vcf2snv/snvAlignment.phy`: an alignment of SNVs used to generate the phylogenetic tree +- `verifying/mappingQuality.txt`: describes how well the given reads mapped to the reference genome + +For more detailed information, please refer to the [SNVPhyl Documentation](https://snvphyl.readthedocs.io/en/latest/user/output/). + +## IRIDA Next Integration File + +A JSON file for loading the data into IRIDA Next is output by this pipeline. The format of this JSON file is specified in our [Pipeline Standards for the IRIDA Next JSON](https://github.com/phac-nml/pipeline-standards#32-irida-next-json). This JSON file is written directly within the `--outdir` provided to the pipeline with the name `irida.output.json.gz` (ex: `[outdir]/irida.output.json.gz`). + +``` +{ + "files": { + "global": [ + { + "path": "make/snvMatrix.tsv" + }, + { + "path": "filter/filterStats.txt" + }, + { + "path": "phyml/phylogeneticTreeStats.txt" + }, + { + "path": "phyml/phylogeneticTree.newick" + }, + { + "path": "vcf2snv/snvTable.tsv" + }, + { + "path": "vcf2snv/vcf2core.tsv" + }, + { + "path": "vcf2snv/snvAlignment.phy" + }, + { + "path": "verifying/mappingQuality.txt" + } + ], + "samples": { + + } + }, + "metadata": { + "samples": { + + } + } +} +``` + +Within the `files` section of this JSON file, all of the output paths are relative to the `--outdir results`. Therefore, `"path": "phyml/phylogeneticTree.newick"` refers to a file located within `results/phyml/phylogeneticTree.newick`. # Legal From 1c01163c4239f557fb4898a2417ad1e08c3beab9 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 12:06:14 -0600 Subject: [PATCH 088/113] Fixing typos. --- README.md | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/README.md b/README.md index 9b4c1a1..d47d27c 100644 --- a/README.md +++ b/README.md @@ -6,7 +6,7 @@ This is the [nf-core](https://nf-co.re/)-based pipeline for [SNVPhyl](https://sn # Input -Input is provided to SNVPhyl in the form of a sample sheet (passed as `--input samplesheet.csv`). This samplesheet is a CSV-formated file, which may be provided as a URI (ex: a file path or web address), and has the following format: +Input is provided to SNVPhyl in the form of a samplesheet (passed as `--input samplesheet.csv`). This samplesheet is a CSV-formated file, which may be provided as a URI (ex: a file path or web address), and has the following format: | sample | fastq_1 | fastq_2 | assembly | |---------|----------------------------|----------------------------|------------------------------| @@ -15,10 +15,10 @@ Input is provided to SNVPhyl in the form of a sample sheet (passed as `--input s The columns are defined as follows: -- sample: The unique sample identifier to associate with the reads (and optionally the assembly). -- fastq_1: A URI (ex: a file path or web address) to either single-end FASTQ-formatted reads or one pair of pair-end FASTQ-formatted reads. -- fastq_2: (Optional) If `fastq_1` is paired-end, then this field is a URI to reads that are the other pair of reads associated with `fastq_1`. -- assembly: (Optional) A URI to an assembly associated with the sample, so that it may be referenced on the command line by the sample identifier for use as the reference for the whole pipeline. However, it may be easier to leave these fields blank and specify the reference using the `--refgenome` parameter. +- `sample`: The unique sample identifier to associate with the reads (and optionally the assembly). +- `fastq_1`: A URI (ex: a file path or web address) to either single-end FASTQ-formatted reads or one pair of pair-end FASTQ-formatted reads. +- `fastq_2`: (Optional) If `fastq_1` is paired-end, then this field is a URI to reads that are the other pair of reads associated with `fastq_1`. +- `assembly`: (Optional) A URI to an assembly associated with the sample, so that it may be referenced on the command line by the sample identifier for use as the reference for the whole pipeline. However, it may be easier to leave these fields blank and specify the reference using the `--refgenome` parameter. The structure of this file is defined in [assets/schema_input.json](assets/schema_input.json). Please see [assets/samplesheet.csv](assets/samplesheet.csv) to see an example of a samplesheet for this pipeline. @@ -66,7 +66,7 @@ In order to run the pipeline with provided data, please run: nextflow run main.nf -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results ``` -The pipeline output will be written to a directory named `results`. A JSON file for integrating data with IRIDA Next will be written to `results/iridanext.output.json.gz` (please see the (Output)[#Output] section for details). +The pipeline output will be written to a directory named `results`. A JSON file for integrating data with IRIDA Next will be written to `results/iridanext.output.json.gz` (please see the Output section for details). # Output From b0a83d4c42d2315a17e928d21b43369af6f2720c Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 12:32:25 -0600 Subject: [PATCH 089/113] Updating docs: README, usage. --- docs/README.md | 2 +- docs/usage.md | 37 +++++++++++++++---------------------- 2 files changed, 16 insertions(+), 23 deletions(-) diff --git a/docs/README.md b/docs/README.md index ef09a38..2e2af69 100644 --- a/docs/README.md +++ b/docs/README.md @@ -7,4 +7,4 @@ The phac-nml/snvphylnfc documentation is split into the following pages: - [Output](output.md) - An overview of the different results produced by the pipeline and how to interpret them. -You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re) +You can find more information about SNVPhyl by reading the [SNVPhyl Documentation](https://snvphyl.readthedocs.io/en/latest/). diff --git a/docs/usage.md b/docs/usage.md index 8d65ce1..d0932ea 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -2,11 +2,11 @@ ## Introduction -This pipeline is an example that illustrates running a nf-core-compliant pipeline on IRIDA Next. +This is the [nf-core](https://nf-co.re/)-based pipeline for [SNVPhyl](https://snvphyl.readthedocs.io/en/). The SNVPhyl (Single Nucleotide Variant PHYLogenomics) pipeline identifies Single Nucleotide Variants (SNV) within a collection of microbial genomes and constructs a phylogenetic tree from those SNVs. ## Samplesheet input -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row as shown in the examples below. ```bash --input '[path to samplesheet file]' @@ -14,22 +14,23 @@ You will need to create a samplesheet with information about the samples you wou ### Full samplesheet -The input samplesheet must contain three columns: `ID`, `fastq_1`, `fastq_2`. The IDs within a samplesheet should be unique. All other columns will be ignored. +The input samplesheet must contain three columns: `sample`, `fastq_1`, `fastq_2`, and `assembly`. The sample IDs within a samplesheet should be unique. A final samplesheet file consisting of both single- and paired-end data may look something like the one below. ```console -sample,fastq_1,fastq_2 -SAMPLE1,sample1_R1.fastq.gz,sample1_R2.fastq.gz -SAMPLE2,sample2_R1.fastq.gz,sample2_R2.fastq.gz -SAMPLE3,sample1_R1.fastq.gz, +| sample | fastq_1 | fastq_2 | assembly | +|---------|----------------------------|----------------------------|------------------------------| +| SAMPLE1 | /path/to/sample1_fastq1.fq | /path/to/sample1_fastq2.fq | /path/to/sample1_assembly.fa | +| SAMPLE2 | /path/to/sample2_fastq1.fq | | | ``` | Column | Description | | --------- | -------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. Samples should be unique within a samplesheet. | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `sample` | Custom sample name. Samples should be unique within a samplesheet. | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `assembly` | (Optional) Full path to a FASTA file representing an assembly derived from this sample. This field provides a method for selecting a reference genome for the whole pipeline. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. @@ -38,10 +39,10 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run main.nf --input ./samplesheet.csv --outdir ./results -profile singularity +nextflow run main.nf -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results ``` -This will launch the pipeline with the `singularity` configuration profile. See below for more information about profiles. +This will launch the pipeline with the `singularity` configuration profile. Note that the pipeline will create the following files in your working directory: @@ -57,7 +58,6 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). -::: The above pipeline run specified with a params file in yaml format: @@ -68,13 +68,12 @@ nextflow run phac-nml/snvphylnfc -profile docker -params-file params.yaml with `params.yaml` containing: ```yaml -input: './samplesheet.csv' +input: 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv' outdir: './results/' +refgenome: 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta' <...> ``` -You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch). - ### Reproducibility It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. @@ -154,12 +153,6 @@ A pipeline might not always support every possible argument or option of a parti To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website. -### nf-core/configs - -In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. - -See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files. - ## Azure Resource Requests To be used with the `azurebatch` profile by specifying the `-profile azurebatch`. From 5810bc92f4aaf3d396d54c0c18198b489897f40e Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 12:48:49 -0600 Subject: [PATCH 090/113] Updating docs/output to be updated, but minimal. --- docs/output.md | 113 +++++++++++++++---------------------------------- 1 file changed, 35 insertions(+), 78 deletions(-) diff --git a/docs/output.md b/docs/output.md index 7f67ff0..1761f7f 100644 --- a/docs/output.md +++ b/docs/output.md @@ -6,86 +6,43 @@ This document describes the output produced by the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. -- assembly: very small mock assembly files for each sample -- generate: intermediate files used in generating the IRIDA Next JSON output -- pipeline_info: information about the pipeline's execution -- simplify: simplified intermediate files used in generating the IRIDA Next JSON output -- summary: summary report about the pipeline's execution and results - -The IRIDA Next-compliant JSON output file will be named `iridanext.output.json.gz` and will be written to the top-level of the results directory. This file is compressed using GZIP and conforms to the [IRIDA Next JSON output specifications](https://github.com/phac-nml/pipeline-standards#42-irida-next-json). +- `bcftools` +- `bgzip` +- `cat` +- `consolidate` +- `filter` +- `find` +- `freebayes` +- `indexing` +- `make` +- `mpileup` +- `phyml` +- `pipeline_info` +- `smalt` +- `sort` +- `vcf2snv` +- `verifying` + +The IRIDA Next-compliant JSON output file will be named `iridanext.output.json.gz` and will be written to the top-level of the results directory. This file is compressed using GZIP and conforms to the [IRIDA Next JSON output specifications](https://github.com/phac-nml/pipeline-standards). ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [Assembly stub](#assembly-stub) - Performs a stub assembly by generating a mock assembly -- [Generate sample JSON](#generate-sample-json) - Generates a JSON file for each sample -- [Generate summary](#generate-summary) - Generates a summary text file describing the samples and assemblies -- [Simplify IRIDA JSON](#simplify-irida-json) - Simplifies the sample JSONs by limiting nesting depth -- [IRIDA Next Output](#irida-next-output) - Generates a JSON output file that is compliant with IRIDA Next -- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution - -### Assembly stub - -

-Output files - -- `assembly/` - - Mock assembly files: `ID.assembly.fa.gz` - -
- -### Generate sample JSON - -
-Output files - -- `generate/` - - JSON files: `ID.json.gz` - -
- -### Generate summary - -
-Output files - -- `summary/` - - Text summary describing samples and assemblies: `summary.txt.gz` - -
- -### Simplify IRIDA JSON - -
-Output files - -- `simplify/` - - Simplified JSON files: `ID.simple.json.gz` - -
- -### IRIDA Next Output - -
-Output files - -- `/` - - IRIDA Next-compliant JSON output: `iridanext.output.json.gz` - -
- -### Pipeline information - -
-Output files - -- `pipeline_info/` - - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. - - Parameters used by the pipeline run: `params.json`. - -
- -[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. +- Indexing +- Find repeats +- SMALT map +- Sort index BAMs +- Verifying map quality +- Freebayes +- Filter freebayes +- BGZIP Freebayes VCFs +- Freebayes VCF to BCF +- mpileup +- bcftools call +- Consolidate BCFs +- CAT +- VCF2SNV alignment +- Filter stats +- PhyML +- Make SNV From defa1fb48b8ed0e56214b50d4bd52b1dfa1cca0a Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 12:58:07 -0600 Subject: [PATCH 091/113] Fixing samplesheet example. --- docs/usage.md | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index d0932ea..86ef3f1 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -19,10 +19,9 @@ The input samplesheet must contain three columns: `sample`, `fastq_1`, `fastq_2` A final samplesheet file consisting of both single- and paired-end data may look something like the one below. ```console -| sample | fastq_1 | fastq_2 | assembly | -|---------|----------------------------|----------------------------|------------------------------| -| SAMPLE1 | /path/to/sample1_fastq1.fq | /path/to/sample1_fastq2.fq | /path/to/sample1_assembly.fa | -| SAMPLE2 | /path/to/sample2_fastq1.fq | | | +sample,fastq_1,fastq_2,assembly +SAMPLE1,/path/to/sample1_fastq1.fq,/path/to/sample1_fastq2.fq,/path/to/sample1_assembly.fa +SAMPLE2,/path/to/sample2_fastq1.fq,, ``` | Column | Description | From 2ee76c1b45804d9ede273d6c37f9c6c2fe89c12f Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 13:06:06 -0600 Subject: [PATCH 092/113] prettier --- README.md | 6 +++--- docs/usage.md | 10 +++++----- 2 files changed, 8 insertions(+), 8 deletions(-) diff --git a/README.md b/README.md index d47d27c..e4ea5ca 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ This is the [nf-core](https://nf-co.re/)-based pipeline for [SNVPhyl](https://sn Input is provided to SNVPhyl in the form of a samplesheet (passed as `--input samplesheet.csv`). This samplesheet is a CSV-formated file, which may be provided as a URI (ex: a file path or web address), and has the following format: | sample | fastq_1 | fastq_2 | assembly | -|---------|----------------------------|----------------------------|------------------------------| +| ------- | -------------------------- | -------------------------- | ---------------------------- | | SAMPLE1 | /path/to/sample1_fastq1.fq | /path/to/sample1_fastq2.fq | /path/to/sample1_assembly.fa | | SAMPLE2 | /path/to/sample2_fastq1.fq | | | @@ -119,12 +119,12 @@ A JSON file for loading the data into IRIDA Next is output by this pipeline. The } ], "samples": { - + } }, "metadata": { "samples": { - + } } } diff --git a/docs/usage.md b/docs/usage.md index 86ef3f1..f93f4ce 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -24,11 +24,11 @@ SAMPLE1,/path/to/sample1_fastq1.fq,/path/to/sample1_fastq2.fq,/path/to/sample1_a SAMPLE2,/path/to/sample2_fastq1.fq,, ``` -| Column | Description | -| --------- | -------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. Samples should be unique within a samplesheet. | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| Column | Description | +| ---------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | +| `sample` | Custom sample name. Samples should be unique within a samplesheet. | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `assembly` | (Optional) Full path to a FASTA file representing an assembly derived from this sample. This field provides a method for selecting a reference genome for the whole pipeline. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From 9aec0993b84e14a93f2f2eab6792ad3298f0a880 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 13:52:18 -0600 Subject: [PATCH 093/113] Updating citations. --- CITATIONS.md | 8 ++++++++ README.md | 11 +++++++++++ 2 files changed, 19 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index c1694ea..537deb4 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,5 +1,13 @@ # phac-nml/snvphylnfc: Citations +## [SNVPhyl](https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000116) + +> Petkau A, Mabon P, Sieffert C, Knox N, Cabral J, Iskander M, Iskander M, Weedmark K, Zaheer R, Katz L, Nadon C, Reimer A, Taboada E, Beiko R, Hsiao W, Brinkman F, Graham M, Van Domselaar G. SNVPhyl: a single nucleotide variant phylogenomics pipeline for microbial genomic epidemiology. 08/06/2017. M Gen 3(6): doi:10.1099/mgen.0.000116. + +## [SNVPhyl Nextflow](https://github.com/DHQP/SNVPhyl_Nextflow) + +> Hagey J. SNVPhyl Nextflow. 2022-21-4. https://github.com/DHQP/SNVPhyl_Nextflow/. + ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) > Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. diff --git a/README.md b/README.md index e4ea5ca..d22442e 100644 --- a/README.md +++ b/README.md @@ -134,6 +134,17 @@ Within the `files` section of this JSON file, all of the output paths are relati # Legal +## nf-core + +This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/master/LICENSE). + +> The nf-core framework for community-curated bioinformatics pipelines. +> +> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. +> +> Nat Biotechnol. 2020 Feb 13. doi: 10.1038/s41587-020-0439-x. +> In addition, references of tools and data used in this pipeline are as follows: + ## SNVPhyl NF-Core Pipeline Copyright 2023 Government of Canada From 19be15205924c6e8522987df6402c7a50e907e78 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 13:54:17 -0600 Subject: [PATCH 094/113] Mandatory reference. --- README.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/README.md b/README.md index d22442e..40edd0e 100644 --- a/README.md +++ b/README.md @@ -31,6 +31,8 @@ The mandatory parameters are as follows: - `--input`: a URI to the samplesheet - `--output`: the directory for pipeline output +Additionally, it is mandatory to have one of either `--refgenome` or `--reference_sample_id` (but not both) to specify the reference. Please see the Reference section for more details. + ## Optional The optional parameters are as follows: From f634f0209546ea4c89a85904cc07f1d79a7823d0 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 13:54:57 -0600 Subject: [PATCH 095/113] removing 'and be included in the analysis'. --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 40edd0e..a087437 100644 --- a/README.md +++ b/README.md @@ -48,8 +48,8 @@ Please use only one of `--refgenome` or `--reference_sample_id` and not both. - `--window_size`: The window size for determining whether a region is high density. - `--density_threshold`: The minimum number of SNVs within the window size for a region to be considered high density. -- `--min_coverage_depth`: The minimum depth of coverage for a position within the genome to pass the mapping quality check and be included in the analysis. -- `--min_mapping_percent_cov`: The total percentage of positions within the genome that must have a depth of coverage greater than the minimum depth of coverage specified in order to pass the mapping quality check and be included in the analysis. +- `--min_coverage_depth`: The minimum depth of coverage for a position within the genome to pass the mapping quality check. +- `--min_mapping_percent_cov`: The total percentage of positions within the genome that must have a depth of coverage greater than the minimum depth of coverage specified in order to pass the mapping quality check. - `--min_mean_mapping_quality`: The minimum mean mapping quality score for all reads in a pileup to be included in the analysis. - `--snv_abundance_ratio`: The proportion of reads required to support a variant to be included in the analysis. - `--min_repeat_length`: The minimum length when identifying repeats. From 2be4bd2038d29cb4e55e43ed46e76c0d65f6b9b4 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 13:55:39 -0600 Subject: [PATCH 096/113] adding 'on the reference genome'. --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index a087437..4f2ea9e 100644 --- a/README.md +++ b/README.md @@ -52,8 +52,8 @@ Please use only one of `--refgenome` or `--reference_sample_id` and not both. - `--min_mapping_percent_cov`: The total percentage of positions within the genome that must have a depth of coverage greater than the minimum depth of coverage specified in order to pass the mapping quality check. - `--min_mean_mapping_quality`: The minimum mean mapping quality score for all reads in a pileup to be included in the analysis. - `--snv_abundance_ratio`: The proportion of reads required to support a variant to be included in the analysis. -- `--min_repeat_length`: The minimum length when identifying repeats. -- `--min_repeat_pid`: The minimum percent identity when identifying repeats. +- `--min_repeat_length`: The minimum length when identifying repeats on the reference genome. +- `--min_repeat_pid`: The minimum percent identity when identifying repeats on the reference genome. - `--skip_density_filter`: Whether or not to skip filtering low SNV density regions. Please refer to the [SNVPhyl documentation](https://snvphyl.readthedocs.io/en/latest/) for more detailed information about pipeline parameters. From a114e651bc85254cc30ff1c79174611979206d8c Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 14:02:37 -0600 Subject: [PATCH 097/113] test profile, profile and revision parameters --- README.md | 11 +++++++++++ 1 file changed, 11 insertions(+) diff --git a/README.md b/README.md index 4f2ea9e..dcf9696 100644 --- a/README.md +++ b/README.md @@ -58,6 +58,11 @@ Please use only one of `--refgenome` or `--reference_sample_id` and not both. Please refer to the [SNVPhyl documentation](https://snvphyl.readthedocs.io/en/latest/) for more detailed information about pipeline parameters. +### Other Parameters + +- `-profile`: specifies which profiles to use (ex: `-profile singularity`) +- `-r`: specifies which revision to use (ex: `-r dev`) + # Running ## Test Data @@ -70,6 +75,12 @@ nextflow run main.nf -profile singularity --input https://raw.githubusercontent. The pipeline output will be written to a directory named `results`. A JSON file for integrating data with IRIDA Next will be written to `results/iridanext.output.json.gz` (please see the Output section for details). +It is also possible to run the pipeline using the test profile as follows: + +``` +nextflow run phac-nml/snvphylnfc -profile singularity,test --outdir results +``` + # Output ## Results From f9d87fcfb004ec10792bdce136ab155ccba31fd3 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 14:03:02 -0600 Subject: [PATCH 098/113] main.nf to phac-nml/snvphylnfc --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index dcf9696..55b66c6 100644 --- a/README.md +++ b/README.md @@ -70,7 +70,7 @@ Please refer to the [SNVPhyl documentation](https://snvphyl.readthedocs.io/en/la In order to run the pipeline with provided data, please run: ``` -nextflow run main.nf -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results +nextflow run phac-nml/snvphylnfc -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results ``` The pipeline output will be written to a directory named `results`. A JSON file for integrating data with IRIDA Next will be written to `results/iridanext.output.json.gz` (please see the Output section for details). From d47cead498a1fe3ccccfc06977f4a9a19f97afd2 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 14:03:40 -0600 Subject: [PATCH 099/113] irida to iridanext --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 55b66c6..7764e96 100644 --- a/README.md +++ b/README.md @@ -100,7 +100,7 @@ For more detailed information, please refer to the [SNVPhyl Documentation](https ## IRIDA Next Integration File -A JSON file for loading the data into IRIDA Next is output by this pipeline. The format of this JSON file is specified in our [Pipeline Standards for the IRIDA Next JSON](https://github.com/phac-nml/pipeline-standards#32-irida-next-json). This JSON file is written directly within the `--outdir` provided to the pipeline with the name `irida.output.json.gz` (ex: `[outdir]/irida.output.json.gz`). +A JSON file for loading the data into IRIDA Next is output by this pipeline. The format of this JSON file is specified in our [Pipeline Standards for the IRIDA Next JSON](https://github.com/phac-nml/pipeline-standards#32-irida-next-json). This JSON file is written directly within the `--outdir` provided to the pipeline with the name `iridanext.output.json.gz` (ex: `[outdir]/iridanext.output.json.gz`). ``` { From 887e53de4eb3cbbd83f6cb0d881e4bbd1e26e931 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 14:04:27 -0600 Subject: [PATCH 100/113] main.nf to phac-nml/snvphylnfc --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index f93f4ce..bef008f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -38,7 +38,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run main.nf -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results +nextflow run phac-nml/snvphylnfc -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results ``` This will launch the pipeline with the `singularity` configuration profile. From be65dc6471674c878b55e94fb96a937a372b5d74 Mon Sep 17 00:00:00 2001 From: Eric Date: Thu, 8 Feb 2024 14:08:57 -0600 Subject: [PATCH 101/113] prettier --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 7764e96..7dc2b5f 100644 --- a/README.md +++ b/README.md @@ -150,7 +150,7 @@ Within the `files` section of this JSON file, all of the output paths are relati ## nf-core This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/master/LICENSE). - + > The nf-core framework for community-curated bioinformatics pipelines. > > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. From db66e67d6d90f101343f4f07262b8872e2c03a43 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 12 Feb 2024 14:05:24 -0600 Subject: [PATCH 102/113] Updating samplesheet. --- assets/samplesheet.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 7708c95..6aefc71 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,4 +1,4 @@ -sample,fastq_1,fastq_2,assembly +sample,fastq_1,fastq_2,reference_assembly SAMPLE1,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_2.fastq,https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta SAMPLE2,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_2.fastq, SAMPLE3,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_2.fastq, From 7feedd37f98d35a3e46b6e702bd4c5f3e6f63b6b Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 12 Feb 2024 14:14:14 -0600 Subject: [PATCH 103/113] assembly -> reference_assembly --- README.md | 8 ++++---- assets/schema_input.json | 4 ++-- docs/usage.md | 6 +++--- tests/workflows/snvphylnfc.nf.test | 8 ++++---- workflows/snvphylnfc.nf | 16 ++++++++-------- 5 files changed, 21 insertions(+), 21 deletions(-) diff --git a/README.md b/README.md index 7dc2b5f..a76522a 100644 --- a/README.md +++ b/README.md @@ -8,17 +8,17 @@ This is the [nf-core](https://nf-co.re/)-based pipeline for [SNVPhyl](https://sn Input is provided to SNVPhyl in the form of a samplesheet (passed as `--input samplesheet.csv`). This samplesheet is a CSV-formated file, which may be provided as a URI (ex: a file path or web address), and has the following format: -| sample | fastq_1 | fastq_2 | assembly | +| sample | fastq_1 | fastq_2 | reference_assembly | | ------- | -------------------------- | -------------------------- | ---------------------------- | | SAMPLE1 | /path/to/sample1_fastq1.fq | /path/to/sample1_fastq2.fq | /path/to/sample1_assembly.fa | | SAMPLE2 | /path/to/sample2_fastq1.fq | | | The columns are defined as follows: -- `sample`: The unique sample identifier to associate with the reads (and optionally the assembly). +- `sample`: The unique sample identifier to associate with the reads (and optionally the reference assembly). - `fastq_1`: A URI (ex: a file path or web address) to either single-end FASTQ-formatted reads or one pair of pair-end FASTQ-formatted reads. - `fastq_2`: (Optional) If `fastq_1` is paired-end, then this field is a URI to reads that are the other pair of reads associated with `fastq_1`. -- `assembly`: (Optional) A URI to an assembly associated with the sample, so that it may be referenced on the command line by the sample identifier for use as the reference for the whole pipeline. However, it may be easier to leave these fields blank and specify the reference using the `--refgenome` parameter. +- `reference_assembly`: (Optional) A URI to a reference assembly associated with the sample, so that it may be referenced on the command line by the sample identifier for use as the reference for the whole pipeline. However, it may be easier to leave these fields blank and specify the reference using the `--refgenome` parameter. The structure of this file is defined in [assets/schema_input.json](assets/schema_input.json). Please see [assets/samplesheet.csv](assets/samplesheet.csv) to see an example of a samplesheet for this pipeline. @@ -40,7 +40,7 @@ The optional parameters are as follows: ### Reference - `--refgenome`: a URI to the reference genome to use during pipeline analysis -- `--reference_sample_id`: the sample identifier of a sample in the samplesheet that contains a provided `assembly` to use as a reference genome during pipeline analysis +- `--reference_sample_id`: the sample identifier of a sample in the samplesheet that contains a provided `reference_assembly` to use as a reference genome during pipeline analysis Please use only one of `--refgenome` or `--reference_sample_id` and not both. diff --git a/assets/schema_input.json b/assets/schema_input.json index a7f1bca..1354fe4 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -34,8 +34,8 @@ } ] }, - "assembly": { - "errorMessage": "Assembly file associated with this sample and must have the extension: '.fa', '.fasta', '.fa.gz' or '.fasta.gz'", + "reference_assembly": { + "errorMessage": "Reference assembly file associated with this sample and must have the extension: '.fa', '.fasta', '.fa.gz' or '.fasta.gz'", "anyOf": [ { "type": "string", diff --git a/docs/usage.md b/docs/usage.md index bef008f..887ea2c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -14,12 +14,12 @@ You will need to create a samplesheet with information about the samples you wou ### Full samplesheet -The input samplesheet must contain three columns: `sample`, `fastq_1`, `fastq_2`, and `assembly`. The sample IDs within a samplesheet should be unique. +The input samplesheet must contain three columns: `sample`, `fastq_1`, `fastq_2`, and `reference_assembly`. The sample IDs within a samplesheet should be unique. A final samplesheet file consisting of both single- and paired-end data may look something like the one below. ```console -sample,fastq_1,fastq_2,assembly +sample,fastq_1,fastq_2,reference_assembly SAMPLE1,/path/to/sample1_fastq1.fq,/path/to/sample1_fastq2.fq,/path/to/sample1_assembly.fa SAMPLE2,/path/to/sample2_fastq1.fq,, ``` @@ -29,7 +29,7 @@ SAMPLE2,/path/to/sample2_fastq1.fq,, | `sample` | Custom sample name. Samples should be unique within a samplesheet. | | `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `assembly` | (Optional) Full path to a FASTA file representing an assembly derived from this sample. This field provides a method for selecting a reference genome for the whole pipeline. | +| `reference_assembly` | (Optional) Full path to a FASTA file representing a reference assembly derived from this sample. This field provides a method for selecting a reference genome for the whole pipeline. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index 4e65bac..8a7aa4a 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -8,7 +8,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" } @@ -78,7 +78,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" reference_sample_id = "SAMPLE1" outdir = "results" } @@ -148,7 +148,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" outdir = "results" } workflow {} @@ -165,7 +165,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" skip_density_filter = true diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index be2b41d..d91a71b 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -87,17 +87,17 @@ workflow SNVPHYL { // NB: `input` corresponds to `params.input` and associated sample sheet schema input = Channel.fromSamplesheet("input") // Map the inputs so that they conform to the nf-core-expected "reads" format. - // Either [meta, [fastq_1], assembly] - // or [meta, [fastq_1, fastq_2], assembly] if fastq_2 exists - .map { meta, fastq_1, fastq_2, assembly -> - fastq_2 ? tuple(meta, [ file(fastq_1), file(fastq_2) ], assembly) : - tuple(meta, [ file(fastq_1) ], file(assembly))} + // Either [meta, [fastq_1], reference_assembly] + // or [meta, [fastq_1, fastq_2], reference_assembly] if fastq_2 exists + .map { meta, fastq_1, fastq_2, reference_assembly -> + fastq_2 ? tuple(meta, [ file(fastq_1), file(fastq_2) ], reference_assembly) : + tuple(meta, [ file(fastq_1) ], file(reference_assembly))} // Channel of read tuples (meta, [fastq_1, fastq_2*]): - reads = input.map { meta, reads, assembly -> tuple(meta, reads) } + reads = input.map { meta, reads, reference_assembly -> tuple(meta, reads) } // Channel of sample tuples (sample ID, assembly): - sample_assemblies = input.map { meta, reads, assembly -> tuple(meta.id, assembly ? assembly : null) } + sample_assemblies = input.map { meta, reads, reference_assembly -> tuple(meta.id, reference_assembly ? reference_assembly : null) } reference_genome = select_reference(params.refgenome, params.reference_sample_id, sample_assemblies) @@ -232,7 +232,7 @@ def select_reference(refgenome, reference_sample_id, sample_assemblies) { } else if (reference_sample_id) { reference_genome = sample_assemblies.filter { it[0] == reference_sample_id && it[1] != null} - .ifEmpty { error("The provided reference sample ID (${reference_sample_id}) is either missing or has no associated assembly.") } + .ifEmpty { error("The provided reference sample ID (${reference_sample_id}) is either missing or has no associated reference assembly.") } .map { it[1] } .first() log.debug "Selecting reference genome ${reference_genome} from '--reference_sample_id'." From ae1b28394ca6e54afe5b0d2d26d8bc6c0ae58abc Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 12 Feb 2024 14:37:04 -0600 Subject: [PATCH 104/113] vcf2snv single->high --- modules/local/vcf2snvalignment/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/vcf2snvalignment/main.nf b/modules/local/vcf2snvalignment/main.nf index f86ff6a..cb68ba4 100644 --- a/modules/local/vcf2snvalignment/main.nf +++ b/modules/local/vcf2snvalignment/main.nf @@ -10,7 +10,7 @@ Please refer to the README for more information. def VERSION = '1.8.2' // Version information not provided by tool on CLI process VCF2SNV_ALIGNMENT { - label 'process_single' + label 'process_high' container = "staphb/snvphyl-tools:1.8.2" input: From 7b24e776b77c82fe96c39a957144f73bb0d127ad Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 12 Feb 2024 14:53:03 -0600 Subject: [PATCH 105/113] Compressed VCFs --- modules/local/mpileup/main.nf | 6 +++--- workflows/snvphylnfc.nf | 7 +------ 2 files changed, 4 insertions(+), 9 deletions(-) diff --git a/modules/local/mpileup/main.nf b/modules/local/mpileup/main.nf index 2beb7d8..8e7b684 100644 --- a/modules/local/mpileup/main.nf +++ b/modules/local/mpileup/main.nf @@ -17,13 +17,13 @@ process MPILEUP { path(refgenome) output: - tuple val(meta), path( "${meta.id}_mpileup.vcf" ), emit: mpileup - path("versions.yml"), emit: versions + tuple val(meta), path( "${meta.id}_mpileup.vcf.gz" ), emit: mpileup + path("versions.yml"), emit: versions script: def prefix = task.ext.prefix ?: "${meta.id}" """ - bcftools mpileup --threads ${task.cpus} --fasta-ref ${refgenome} -A -B -C 0 -d 1024 -q 0 -Q 0 --output-type v -I --output ${prefix}_mpileup.vcf ${sorted_bams} + bcftools mpileup --threads ${task.cpus} --fasta-ref ${refgenome} -A -B -C 0 -d 1024 -q 0 -Q 0 --output-type z -I --output ${prefix}_mpileup.vcf.gz ${sorted_bams} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index d91a71b..149c06c 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -152,13 +152,8 @@ workflow SNVPHYL { ) ch_versions = ch_versions.mix(MPILEUP.out.versions) - BGZIP_MPILEUP_VCF( - MPILEUP.out.mpileup - ) - ch_versions = ch_versions.mix(BGZIP_MPILEUP_VCF.out.versions) - BCFTOOLS_CALL( - BGZIP_MPILEUP_VCF.out.mpileup_zipped + MPILEUP.out.mpileup ) ch_versions = ch_versions.mix(BCFTOOLS_CALL.out.versions) From b920e34b544e23eca78d30025e15e27a85937f7c Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 12 Feb 2024 14:55:30 -0600 Subject: [PATCH 106/113] Removing old module for compressing mpileup files. --- modules/local/bgzipmpileupvcf/main.nf | 31 --------------------------- 1 file changed, 31 deletions(-) delete mode 100644 modules/local/bgzipmpileupvcf/main.nf diff --git a/modules/local/bgzipmpileupvcf/main.nf b/modules/local/bgzipmpileupvcf/main.nf deleted file mode 100644 index 589fa6f..0000000 --- a/modules/local/bgzipmpileupvcf/main.nf +++ /dev/null @@ -1,31 +0,0 @@ -/* -This source file is adapted from a nf-core pipeline for SNVPhyl developed by -Jill Hagey as a work of the United States Government, which was distributed as -a work within the public domain under the Apache Software License version 2. - -This source file has been adapted to work within our pipeline. - -Please refer to the README for more information. -*/ -process BGZIP_MPILEUP_VCF { - tag "${meta.id}" - label 'process_low' - container = "staphb/htslib:1.15" - - input: - tuple val(meta), path(mpileup_vcf) - - output: - tuple val(meta), path("${meta.id}_mpileup.vcf.gz"), emit: mpileup_zipped - path("versions.yml"), emit: versions - - script: - """ - bgzip -f ${mpileup_vcf} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bgzip (htslib): \$( bgzip --version | head --lines 1 | sed 's/bgzip (htslib) //' ) - END_VERSIONS - """ -} From 8a150440402a6183360e545001b7e7df9b8daddc Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 12 Feb 2024 14:57:34 -0600 Subject: [PATCH 107/113] prettier --- docs/usage.md | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 887ea2c..2849c67 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -24,11 +24,11 @@ SAMPLE1,/path/to/sample1_fastq1.fq,/path/to/sample1_fastq2.fq,/path/to/sample1_a SAMPLE2,/path/to/sample2_fastq1.fq,, ``` -| Column | Description | -| ---------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. Samples should be unique within a samplesheet. | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| Column | Description | +| -------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | +| `sample` | Custom sample name. Samples should be unique within a samplesheet. | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `reference_assembly` | (Optional) Full path to a FASTA file representing a reference assembly derived from this sample. This field provides a method for selecting a reference genome for the whole pipeline. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From 637fd86e59e4f8f12c1dbfea8110ef30e26ce685 Mon Sep 17 00:00:00 2001 From: Eric Date: Mon, 12 Feb 2024 15:06:14 -0600 Subject: [PATCH 108/113] Removing import --- workflows/snvphylnfc.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/workflows/snvphylnfc.nf b/workflows/snvphylnfc.nf index 149c06c..fdadc68 100644 --- a/workflows/snvphylnfc.nf +++ b/workflows/snvphylnfc.nf @@ -53,7 +53,6 @@ include { FILTER_FREEBAYES } from '../modules/local/filterfreebayes/main' include { BGZIP_FREEBAYES_VCF } from '../modules/local/bgzipfreebayesvcf/main' include { FREEBAYES_VCF_TO_BCF } from '../modules/local/freebayesvcftobcf/main' include { MPILEUP } from '../modules/local/mpileup/main' -include { BGZIP_MPILEUP_VCF } from '../modules/local/bgzipmpileupvcf/main' include { BCFTOOLS_CALL } from '../modules/local/bcftoolscall/main' include { CONSOLIDATE_BCFS } from '../modules/local/consolidatebcfs/main' include { VCF2SNV_ALIGNMENT } from '../modules/local/vcf2snvalignment/main' From 611667a253dcb58ab4d6c9d1cf27abb3644bc291 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 14 Feb 2024 09:42:12 -0600 Subject: [PATCH 109/113] Updating default parameters. --- nextflow.config | 4 ++-- nextflow_schema.json | 4 ++-- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/nextflow.config b/nextflow.config index 978e9a4..3c1004d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -15,9 +15,9 @@ params { reference_sample_id = null // Pipeline parameters - window_size = 11 + window_size = 500 density_threshold = 2 - min_coverage_depth = 5 + min_coverage_depth = 15 min_mapping_percent_cov = 80 min_mean_mapping_quality = 30 snv_abundance_ratio = 0.75 diff --git a/nextflow_schema.json b/nextflow_schema.json index f768dc2..378c293 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -68,7 +68,7 @@ "properties": { "min_coverage_depth": { "type": "integer", - "default": 5, + "default": 15, "fa_icon": "fas fa-desktop", "description": "The minimum depth of coverage for a position within the genome to pass the mapping quality check and be included in the analysis.", "minimum": 1 @@ -90,7 +90,7 @@ }, "window_size": { "type": "integer", - "default": 11, + "default": 500, "description": "The window size for determining whether a region is high density.", "fa_icon": "fas fa-desktop", "minimum": 1 From bfb8116687e5a06c7851a381fa426ecb2c418638 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 14 Feb 2024 10:00:06 -0600 Subject: [PATCH 110/113] Version numbers. --- CHANGELOG.md | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index cd2cdce..862c239 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,9 +3,9 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 1.0.0dev +## 1.0.0 - 2024/02/14 -Development release of phac-nml/snvphylnfc. +The initial release of phac-nml/snvphylnfc. ### `Added` diff --git a/nextflow.config b/nextflow.config index 3c1004d..f1bb503 100644 --- a/nextflow.config +++ b/nextflow.config @@ -208,7 +208,7 @@ manifest { description = """IRIDA Next Example Pipeline""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0.0dev' + version = '1.0.0' doi = '' defaultBranch = 'main' } From 0603d0c4c0e9c616d5c1fd713a0e70ca9d31601e Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 14 Feb 2024 10:10:40 -0600 Subject: [PATCH 111/113] Updating links to data. --- README.md | 2 +- assets/samplesheet.csv | 2 +- conf/test.config | 4 ++-- conf/test_full.config | 2 +- docs/usage.md | 4 ++-- tests/modules/local/freebayes/main.nf.test | 6 +++--- tests/modules/local/smaltmap/main.nf.test | 2 +- tests/workflows/snvphylnfc.nf.test | 8 ++++---- 8 files changed, 15 insertions(+), 15 deletions(-) diff --git a/README.md b/README.md index a76522a..7145d53 100644 --- a/README.md +++ b/README.md @@ -70,7 +70,7 @@ Please refer to the [SNVPhyl documentation](https://snvphyl.readthedocs.io/en/la In order to run the pipeline with provided data, please run: ``` -nextflow run phac-nml/snvphylnfc -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results +nextflow run phac-nml/snvphylnfc -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta --outdir results ``` The pipeline output will be written to a directory named `results`. A JSON file for integrating data with IRIDA Next will be written to `results/iridanext.output.json.gz` (please see the Output section for details). diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 6aefc71..16c1ebd 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,4 +1,4 @@ sample,fastq_1,fastq_2,reference_assembly -SAMPLE1,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_2.fastq,https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta +SAMPLE1,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/a_2.fastq,https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta SAMPLE2,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/b_2.fastq, SAMPLE3,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_1.fastq,https://raw.githubusercontent.com/phac-nml/snvphyl-galaxy-cli/development/example-data/fastqs/c_2.fastq, diff --git a/conf/test.config b/conf/test.config index 350ffc7..f7ef9a3 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,6 +20,6 @@ params { max_time = '1.h' // Input data - input = 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/samplesheet.csv' - refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + input = 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv' + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" } diff --git a/conf/test_full.config b/conf/test_full.config index 1eb9b23..bfccaaa 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -15,5 +15,5 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test - input = 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/samplesheet.csv' + input = 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv' } diff --git a/docs/usage.md b/docs/usage.md index 2849c67..4c3f9ae 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -38,7 +38,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run phac-nml/snvphylnfc -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta --outdir results +nextflow run phac-nml/snvphylnfc -profile singularity --input https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv --refgenome https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta --outdir results ``` This will launch the pipeline with the `singularity` configuration profile. @@ -69,7 +69,7 @@ with `params.yaml` containing: ```yaml input: 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv' outdir: './results/' -refgenome: 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta' +refgenome: 'https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta' <...> ``` diff --git a/tests/modules/local/freebayes/main.nf.test b/tests/modules/local/freebayes/main.nf.test index 17cec4a..c10fb04 100644 --- a/tests/modules/local/freebayes/main.nf.test +++ b/tests/modules/local/freebayes/main.nf.test @@ -8,7 +8,7 @@ nextflow_process { when { params { - refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" } process { @@ -43,7 +43,7 @@ nextflow_process { when { params { - refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" } process { @@ -79,7 +79,7 @@ nextflow_process { when { params { - refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" } process { diff --git a/tests/modules/local/smaltmap/main.nf.test b/tests/modules/local/smaltmap/main.nf.test index 7592fa6..c557e23 100644 --- a/tests/modules/local/smaltmap/main.nf.test +++ b/tests/modules/local/smaltmap/main.nf.test @@ -8,7 +8,7 @@ nextflow_process { when { params { - refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/main/assets/reference.fasta" + refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" } process { diff --git a/tests/workflows/snvphylnfc.nf.test b/tests/workflows/snvphylnfc.nf.test index 8a7aa4a..4e65bac 100644 --- a/tests/workflows/snvphylnfc.nf.test +++ b/tests/workflows/snvphylnfc.nf.test @@ -8,7 +8,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" } @@ -78,7 +78,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" reference_sample_id = "SAMPLE1" outdir = "results" } @@ -148,7 +148,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" outdir = "results" } workflow {} @@ -165,7 +165,7 @@ nextflow_workflow { when { params { - input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/db66e67d6d90f101343f4f07262b8872e2c03a43/assets/samplesheet.csv" + input = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/samplesheet.csv" refgenome = "https://raw.githubusercontent.com/phac-nml/snvphylnfc/dev/assets/reference.fasta" outdir = "results" skip_density_filter = true From 0353b9e9d6c21206311b83bd0180f2f0cdc339ca Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 14 Feb 2024 10:38:11 -0600 Subject: [PATCH 112/113] Version 2.0.0 --- CHANGELOG.md | 2 +- nextflow.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 862c239..40cdb3d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 1.0.0 - 2024/02/14 +## 2.0.0 - 2024/02/14 The initial release of phac-nml/snvphylnfc. diff --git a/nextflow.config b/nextflow.config index f1bb503..ec31378 100644 --- a/nextflow.config +++ b/nextflow.config @@ -208,7 +208,7 @@ manifest { description = """IRIDA Next Example Pipeline""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0.0' + version = '2.0.0' doi = '' defaultBranch = 'main' } From cb4a47f1b8e15d2847d76eba810883e1b286fb06 Mon Sep 17 00:00:00 2001 From: Eric Date: Wed, 14 Feb 2024 13:36:08 -0600 Subject: [PATCH 113/113] Changelog, pipeline description. --- CHANGELOG.md | 13 +++++++++---- nextflow.config | 2 +- 2 files changed, 10 insertions(+), 5 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 40cdb3d..03a86ad 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,12 +5,17 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## 2.0.0 - 2024/02/14 -The initial release of phac-nml/snvphylnfc. - ### `Added` -### `Fixed` +- The initial release of phac-nml/snvphylnfc as a Nextflow pipeline follwing nf-core pipeline standards. -### `Dependencies` +### `Changed` + +- Migrated SNVPhyl to a Nextflow pipeline. +- Updated the major SNVPhyl release version from 1 to 2 in order to reflect the migration from a Galaxy-based pipeline to a Nextflow-based pipeline. ### `Deprecated` + +### `Fixed` + +### `Dependencies` diff --git a/nextflow.config b/nextflow.config index ec31378..979441d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -205,7 +205,7 @@ manifest { name = 'phac-nml/snvphylnfc' author = """Aaron Petkau and Eric Marinier""" homePage = 'https://github.com/phac-nml/snvphylnfc' - description = """IRIDA Next Example Pipeline""" + description = """SNVPhyl Pipeline""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '2.0.0'